Showing 2 results for Diabetes Mellitus Type Ii
Bagher Larijani, Masoud Houshmand, Ghamar Soltan Dorraj, Farzaneh Darvishzadeh,
Volume 4, Issue 3 (5-2005)
Abstract
Background: Mitochondria is one of the intracellular organelle with specific DNA. Some diseases caused by mtDNA mutations have been reported up to now. Mutation of A3243G and deletion of 5kb are two of them that related to Diabetes type II. The aim of this study was to evaluate the frequency of A3243G mutation and 5kb mt DNA deletion in type II diabetic patients.
Methods: The DNA extracted from blood of 130 patients with diabetes type II. Serum insulin of the patients were also measured. Sequence assigning, PCR – RFLP and SSCP methods were used to detect the A3243G or other mutation in mitochondrial tRNA (leu) gene. Standard and multiplex PCR were used to detect 5kb deletion in patient’s mt DNA and were compared with 40 healthy persons.
Results :We couldn’t identify any deletion of 5kb or A3243G point mutation in our patients but SSCP results showed new pattern of PCR Product in patients. An “A” nucleotide deletion in A 3314 position was detected in mitochondrial ND1 gene in 6 patients. So far this point deletion has not been reported.
Conclusion: Identification of the mitochondrial gene mutations helps to preclinical diagnosis of diabetes type 2. More research is necessary in this field.
Zahra Sadeghian Far, Abdolkarim Sheikhi, Seyed Mohammad Gheibi Hayat,
Volume 16, Issue 6 (10-2017)
Abstract
Background: Usually excess feeding in stored in the body as fat and leads to overweight. One of the causes of catching type 2 diabetes is obesity. Proinflammatory cytokines such as TNF- α and IFNγ can damageTnf- Α And Ifnγ By Peripheral Blood Mononuclear Cells Of Patients With Diabetes Mellitus Type Ii Admitted To Ganjavian Dezful Hospit insulin signaling in insulin-sensitive tissues. In this study, the effect of garlic extract on the expression of TNF-α and IFNγ secreted by the mononuclear cells was measured.
Methods: After taking peripheral blood from patient with type 2 diabetes, mononuclear cells were extracted by Ficoll Method. Cells were a culture with two different concentration of garlic extract (10% and 30%) for 48 and 24 hours. Then for measuring TNF-α and IFNγ release level, ELISA method was used.
Results: Garlic extract can reduce the amount of TNF-α secretion from cells mononuclear, although the timing and concentration of the extract had no effect on the results. Moreover, garlic extract had no effect on the secretion of IFNγ.
Conclusion: Using garlic extract can be adopted therapeutic strategies aimed at adipose tissue inflammation and insulin resistance that is useful for the prevention and treatment of type 2 diabetes.
