Showing 7 results for Gene Expression
Nosratollah Zarghami, Behrang Alani, Habib Onsori, Akram Tamizi, Mehran Mesgari,
Volume 5, Issue 2 (9-2005)
Abstract
Background: Leptin, a peptide hormone, is the product of "ob" Gene. Leptin regulate body weight and composition through reducing appetite and energy expenditure in rodents and humans. The aim of this study was to evaluate differences in expression of Leptin Gene in different tissues of streptozotocin induced diabetic rats.
Methods: 40 Sprague Dawely rat were selected. Intra peritoneal injection was carried out in 20 rats and another 20 rats were used as control. After injection of 60mg/kg Streptozotocin, animals were transformed into diabetic. Glucose was measured by glucose oxidase method. Leptin and insulin were measure by commercially available immunoassay kits. After one week treatment, different tissues including adipose tissues, Spleen, epidydimis, and Liver of both control and experimental animals were dissected. For investigation of any changes of the Leptin gene expression in different tissues, RNA was extracted using Trizo1 method. By using RT-PCR technique, Leptin cDNA and β-actin cDNA as internal control were constructed and PCR was carried out. The RT-PCR products were detected on 2% agarose gel using electrophoresis.
Results: Mean serum levels of Leptin was 5.23± 0.45 ng/ml before injection of streptozotocin and markedly decreased in STZ induced diabetic rats to 0.79±0.25 ng/ml. This decrease was statistically significant P<0.05). There was a direct and significant correlation between leptin and insulin in streptozotocin-induced diabetic rats (r=0.37, P<0.05 ) while, this was reverse in control rats ( r= -0.28, P<0.05). Using RT-PCR method, Leptin gene expression in different tissues including fat epidydimis, liver, and spleen showed that the intensity of leptin band with 452 bp was decreased in diabetic rats in comparison to normal rats. Actin Gene expression was identified in PCR products having 403 bp and the intensity was constant in both groups. The reduction rates of "ob" mRNA in fat epidydimis tissue in STZ diabetic rats was remarkable in comparison to Spleen and Liver.
Conclusion: It is speculated that Leptin gene could be under regulation of insulin dependent mechanism in diabetic rats and by modulating Leptin gene expression in diabetic patients, it may be useful in clinical practices.
Malihe Aveseh, Rohollah Nikooie, Fereshteh Atabi, Zahra Mirzaie Zadeh , Kobra Omidfar, Bagher Larijani,
Volume 13, Issue 2 (1-2014)
Abstract
Background: the aim of this study was to investigate the effect of seven weeks endurance training on
RBP4 gene expression in Soleus and Extensor Digitrum Longouse (EDL) Muscles, liver, visceral and
subcutaneous fat in type 2 diabetic rats.
Methods: 50 male wistar rats (5 weeks years old, weight = 93.7 ± 8.9) were purchased and randomly
divided into four groups: Control (n=10) (C), Trained (n=10) (T), Diabetic Control (n=15) (DC) and
Trained diabetic (n=15) (TD). Diabetes was induced by injection of low dose of streptozotocin (STZ)
and feeding with high fat diet. Insulin resistance accuracy was confirmed by HOMA-IR index and
Real-time PCR was used for mRNA content.
Results: After seven weeks of diabetes induction, the RBP4 mRNA content of the liver (2.37-fold P <
0.01), visceral fat (2.33-fold P < 0.01), and subcutaneous fat (1.83-fold P < 0.05), soleus (1.21-fold
P < 0.05) and EDL (2.03-fold P < 0.05) were increased. After seven weeks of endurance training
significant decrease in RBP4 mRNA content was found in visceral fat (P < 0.05), subcutaneous fat (P
< 0.05) and EDL (P < 0.05) between DC and CD. In addition, significant difference between T and TD
groups was found for RBP4 mRNA content in liver (p < 0.01), subcutaneous fat (P < 0.01) and EDL
(P < 0.01) after seven weeks of endurance training.
Conclusion: Type 2 diabetes considerably increases skeletal muscle RBP4 expression in isoform-
specific manner. This increase is also seen in liver, subcutaneous and visceral fat. In addition,
endurance training decreases the RBP4 expression in EDL, subcutaneous and visceral fat.
Amirabbas Monazzami, Hamid Rajabi, Kobra Omidfar, Ali Mostafaie,
Volume 13, Issue 5 (7-2014)
Abstract
Background: The purpose of this study was to investigate the effects of endurance training on muscle NHE1 and NBC1 gene expressions in type 2 diabetic rats.
Methods: Male wistar rats (n=40), 4weeks old and 93.7±9.8g, were randomly selected and divided into control, diabetic control and diabetic training groups. The Endurance training was performed for 7 weeks on diabetic training groups (running on treadmill forrodent). NHE1 and NBC1 gene expression were determined by Realtime-PCR technique. The differences between groups in variables were determined by an independent t-test using REST Software.
Results: NHE1 mRNA expression reduced significantly in EDL and Soleus by 25% and 19% in the diabetic control group compared with the control group, respectively (P<0/05).NHE1 mRNA expression also reduced significantly in EDL and Soleus by 35% and 29% in the diabetic control group compared with the control group, respectively (P<0/05).Endurance training increased NHE1 and NBC1 geneexpressions in both EDL and Soleus in the diabetic training group.
Conclusion: The present study showed that NHE1 and NBC1 mRNA expressions decreased significantly in the diabetic control group and endurance training increased NHE1 and NBC1 mRNA expressions in the diabetic trained group leading to normalizing the mRNAs in diabetic trained group.
Maryam Ghorbani, Rozita Fathi, Khadijeh Nasiri, Farhad Ahmadi,
Volume 19, Issue 1 (1-2020)
Abstract
Background: Skeletal muscle as an endocrine tissue is involved in the regulation of metabolic activity, production and secretion of hormones including myokines. The aim of the present study was to investigate the effect of eight weeks of aerobic training combined with ethanol consumption on plasma lipid profile and glucose levels, triglyceride content and mayonectin, irisin and leptin gene expression in the gastrocnemius muscle in male rats.
Methods: A number of 32 rats with a weighing average of 200 ± 10 g were divided into four groups control, aerobic training, ethanol with dose of 4 g/kgbw, and ethanol +aerobic training. At the end of the period, values of lipid profile and plasma glucose, the amount of triglyceride of the gastrocnemius muscle and the relative levels of mayonectin, irisin and leptin gene expression were evaluated. Data were analyzed using two-way ANOVA.
Results: The plasma triglyceride levels in the aerobic training and ethanol+aerobic training groups were significantly decreased compared to the control group (P≤0.05). The gastrocnemius muscle triglyceride values were significantly increased in the ethanol +aerobic training (P≤0.0001) and aerobic training groups (P≤0.01) compared to the control group. The results showed that aerobic training significantly increased myonectin gene expression in aerobic training group (P≤0.05), but the expression of irisin and leptin genes did not change significantly in different groups.
Conclusion: Aerobic training during the eight-week was able to improve lipid content, especially plasma triglyceride and skeletal muscle triglyceride, and possibly regulate body metabolism by altering the levels of myokines, especially myonectin.
Sepideh Salehi, Niku Khosravi, Maryam Delfan,
Volume 19, Issue 5 (6-2020)
Abstract
Background: The prevalence of type 2 diabetes is rapidly increasing in the world. As a result of this disease, long-term insulin resistance develops, as a result of which pancreatic beta cells are destroyed and disappear, as a result insulin is not released. Recently, a protein called lipasin, which is responsible for signaling the liver to beta cells, has been discovered, and previous reports have shown that lipasin/betatrophin increases pancreatic β cell proliferation. Therefore, the aim of this study was to investigate the effect of eight weeks of high intensity interval training (HIIT) and endurance training on lipasin gene expression in rats with type 2 diabetes.
Methods: The study was performed on 25 Wistar rats with a mean weight of 160±10 g and age of 8 weeks. After induction of diabetes, the rats were randomly divided into three groups of 6: control and endurance and HIIT. And exercise was performed for eight weeks (5 sessions per week). QRT-PCR technique was used to evaluate changes in hepatic lipasin gene expression.
Results: The present study showed that after eight weeks of endurance training and HIIT, the expression of lipasin gene in the liver of rats in the training group increased significantly compared to the control group (P = 0.037); Also, a significant negative correlation was observed between lipasin gene expression and insulin resistance index in the exercise group compared to the control group (r = -0.605, P = 0.037).
Conclusion: It seems that performing eight weeks of endurance training and HIIT, by increasing the expression of lipasin gene can increase beta cells in diabetic patients and may be an effective non-pharmacological intervention to reduce the symptoms of this disease.
Mahdi Maghami, Saeed Keshavarz, Rouhollah Haghshenas, Elham Eftekhari,
Volume 21, Issue 4 (10-2021)
Abstract
Background: Diabetes is one of the most common chronic diseases in today's society. The aim of this study was to investigate the effect of endurance training and nettle consumption on the protein and gene expression of AKT, GLUT4 and insulin in male of muscle soleus of diabetic rats.
Methods: After adaptation to the environment, 40 male Wistar rats were divided into 5 groups: control (C), diabetes (D), nettle+diabetes (UD), exercise+diabetes (ED) and exercise+nettle+diabetes (EUD). After developing and confirming the diabetes model by injection of STZ, rats in the exercise groups performed an endurance training protocol for eight weeks and rats in the nettle groups consumed nettle extract 5 days a week. After completion of the protocol and extraction of soleus muscle, RT-PCR method was used to measure gene expression, Western blot and immunohistochemically method was used to measure protein expression of glut-4 and AKT respectively and ELIZA method was used to measure glucose and insulin.
Results: The results showed that the protein and genes expression of GLUT4 and AKT in the diabetic group compared to the control group was significantly reduced (P<0.001) while the expression of these two variables was significantly increased in the ED and EUD groups (P<0.001) that this increase was greater in the EUD group. The effect of nettle alone on the expression of these two variables was not significant. Glucose, insulin and insulin resistance increased significantly in the diabetes group, while these decreased significantly in the ED and EUD groups (P<0.001).
Conclusion: The synergist of the combination of nettle and exercise significantly improves the improve of diabetes, while each alone has little effect on diabetes-related variables.
Monireh Khalili, Habib Asgharpour, Asra Askari, Jahanbakhsh Asadi,
Volume 23, Issue 6 (1-2024)
Abstract
Background: The health and proper functioning of the endoplasmic reticulum in the liver can lead to an increase in the quality of the metabolism of liver cells. Exercise can lead to liver health by regulating liver enzymes and factors related to apoptosis pathway. Therefore, the aim of the current research is to compare the performance of the endoplasmic reticulum chaperone protein of the liver of healthy model rats with the intervention of different training sessions and starvation.
Methods: In this experimental research, 30 Wistar male rats with a weight range of 330 ± 25 grams were selected and divided into six groups: 1) control, 2) starvation, 3) 3 days of training per week, 4) 5 days of training per week, 5) starvation + 3 training days per week, 6) starvation + 5 training days per week. Training consisted of one hour of continuous running on a treadmill for 4 weeks, and starvation was performed for 14 hours when the rats were awake. Data analysis was performed using one-way analysis of variance and post hoc LSD statistical tests in SPSS version 23 software. A significance level of P≤ 0.05 was considered.
Results: Data analysis showed a significant change in the levels of liver enzymes alanine aminotransferase (ALT) and aspartate transaminase (AST) (P≤ 0.05). This change in CHOP gene expression was also significant (P≤ 0.05).
Conclusion: Aerobic training for 3 and 5 days with starvation can lead to liver health by reducing liver enzymes (AST and ALT) and cell death-related factor (CHOP) through the apoptosis pathway.
