Showing 3 results for Immune System
Sedigheh Asgari, Zahra Fattahi, Gholam Ali Naderi, Shirin Azam Panah,
Volume 6, Issue 4 (6-2007)
Abstract
Background: Several studies were pointed to oxidized LDL (OX-LDL) as one of the main immunogenic agents which have important roles in primary lesions of atherosclerosis. In this study, via immunization against OX-LDL with two different antigens in an animal model (rabbit) we tried to clear relation between immune system and atherosclerosis.
Methods: LDL was isolated from healthy human plasma and oxidized with MDA or Cu++. Rabbits were divided to three groups and after 2 weeks (under basic diet) immunized with MDA-LDL or Cu-LDL. In control group Phosphate-buffered saline (PBS) was used. Immunization was repeated with these materials again the weeks of 2, 4, 6, and 8 and concentration of OX-LDL antibody was measured in each stage. At the end of 8th week, rabbits fed normal or high cholesterol regimens. Biochemical factors were measured at the beginning and end of study, also Fatty streaks in aorta and left and right coronary arteries was evaluated.
Results: Immunization with Cu -LDL and MDA-LDL induced adequate antibody formation (IgG) at the end of 8th weeks. Immunization with MDA-LDL significantly decreased the level of cholesterol, LDL-cholesterol and fasting blood sugar (FBS) (P= 0.04). Also a significant decrease in fatty streak lesions was detected in aorta and right and left coronary arteries as compared with non-immunized high-cholesterol group (P= 0.04). Immunization with Cu -LDL significantly decreased Triglyceride, FBS and cholesterol as compared with non-immunized high cholesterol. No differences were detected in the fatty streak lesions in this group as compared with non-immunized high-cholesterol diet cu+2-LDL immunized group, shown significant decrease in CRP as compared with both non-immunized group and high cholesterol diet.
Conclusion: We conclude the protective effects of immune responses on atherosclerosis depend to what kind of antibody, so MDA-LDL and CU-LDL prevent atherosclerosis with different mechanism.
Leila Eini, Negar Naghash, Bagher Larijani, Jafar Ai, Keivan Majidzadeh, Esmaeil Sadroddiny, Kobra Omidfar,
Volume 13, Issue 2 (1-2014)
Abstract
Background: Mesenchymal stem cells (MSCs), also known as mesenchymal stromal cells, are
considered as an important source of adult stem cells in tissue engineering and cell therapy. They are
present in various tissues such as, endometrium as the supportive cells. According to anatomical
position of endometrial mesenchymal stem cells that put them in neighborhood of the fetus, they may
have a significant role in fetus tolerance during pregnancy.
This study was conducted to evaluate the molecular mechanism of immunosuppressive affect of
endometrial mesenchymal stem cells.
Methods: Mesenchymal Cells from bone marrow and endometrium were cultured at density of 2 ×105
cells/ml at presence of 100IU/ml and 500IU/ml INF-γ (IFN-gamma) and expression of indoleamine 2
3-dioxygenase (IDO) were studied after 72 hours by real-time PCR and immunocytochemistry.
Results: The study showed that IDO expression in cells exposed to IFN-γ was increased compared to
the cells in the absence of IFN-γ (p<0.05). Additionally, up regulation of IDO expression was higher
in endometrial cells than bone marrow cells.
Conclusion: From these results it is concluded that endometrial mesenchymal stem cells may be used
as a good candidate for cell therapy.
Behrouz Alizadeh Qaleh Zavaraq, Farzad Zehsaz, Karim Azali Alamdari, Akbar Moin,
Volume 23, Issue 3 (9-2023)
Abstract
Background: The role of the immune system and inflammation in controlling metabolic diseases such as insulin resistance, type 2 diabetes, obesity and overweight is well established. Also, physical activity controls and prevents the occurrence of metabolic diseases, which has anti-inflammatory and anti-oxidative effects. The aim of the present study was to study the effect of eight weeks of 10, 20, 30 seconds exercise training on the levels of antimicrobial factors in the saliva of obese adolescent boys.
Methods: 32 male students voluntarily participated in the present study and were randomly assigned to four groups: 1) obese aerobic exercise, 2) normal weight aerobic exercise, 3) obese control without exercise, and 4) normal weight control without exercise. The criteria for the obese group was a BMI above 25. Anthropometric indices of height, weight and body mass index were measured exactly. Before and after eight weeks of practice, Shatell-Run standard test was performed. Salivary samples of lysozyme, lactoferrin, lactate and C-reactive protein concentrations were taken after eight weeks of training with a frequency of three sessions per week with an intensity of 30 to 90% of maximum aerobic power. Using analysis of covariance, variables with a significance level of less than (P≤0.05) were included in the analysis.
Results: The results showed a significant increase in salivary levels of lactoferrin (P=0.001), lysozyme (P=0.003), lactate (P=0.001), and C-reactive protein (P=0.006). There are more obese students than people of normal weight (Youth body mass index criterion)
Conclusion: The increased response of some antibacterial and inflammatory salivary proteins after eight weeks of 10-20-30 sports training following exhaustive activity may be due to the acute response of the immune system.
