Ali Jalili, Bagher Larijani, Farideh Razi, Ensieh Nasli, Mostafa Qorbani,
Volume 16, Issue 6 (10-2017)
Abstract
Background: Diabetic nephropathy is a chronic kidney disease and of more common complications of type 2 diabetes mellitus. The current diagnostic markers of diabetic nephropathy, albumin and creatinine, are only able to catch the disease in the stage of renal damage. The aim of this study is evaluation of targeted metabolomics of serum amino acids to identify the association of the changes of serum amino acid profile with diabetes and diabetic nephropathy.
Methods: This cross-sectional study was conducted in 2015-2016 on thirty patients with type 2 diabetes subsequent diabetic nephropathy and thirty type 2 diabetic patients without nephropathy attending diabetes clinic of endocrinology and metabolism institute and thirty non diabetic persons. Blood hemoglobin, HbA1c and BUN and also, serum albumin, uric acid and the albumin/creatinine ratio from a random urine specimen were measured by standard methods and serum amino acids level were identified using high performance liquid chromatography (HPLC). Statistical analysis ANOVA, Kruskal-Wallis, and nominal regression were used for the comparison of the investigated groups.
Results: significant differences were seen in serum levels of 8 essential, branched-chains, aromatic and 8 non-essential amino acids alanine, aspartic acid, serine, glutamine, arginine, glycine, tyrosine and ornithine between three groups. Serum levels of arginine and isoleucine were higher in the diabetic group than non-diabetics. However, Levels of amino acids serine, glutamine, glycine, threonine, tyrosine, tryptophan, methionine, valine, ornithine, and lysine in 2 groups of diabetic nephropathy and diabetes were higher than non-diabetic patients.
For every standard deviation decrease in serum levels of amino acids serine, alanine and isoleucine, in comparison to diabetic patients, the risk of diabetic nephropathy were increased 3.257 (95%CI: 0.10- 0.94, P=0.039), 2.207 (95%CI: 0.18- 0.81, P=0.039) and 2.652 (0.21- 0.96, P=0.012), respectively.
Conclusion: Since this study was conducted in patients in the early stages of the disease, reduced serum levels of the amino acids serine, leucine and alanine may be associated with development and progression of diabetic nephropathy. and in the future with more studies in this field can be used in metabolic control and improvement of the prognosis of patients with diabetic nephropathy.
Esmail Shekari, Seyed Kianoosh Hosseini, Farideh Razi, Ensieh Nasli Esfahani, Mostafa Qorbani, Bagher Larijani,
Volume 19, Issue 4 (4-2020)
Abstract
Background: Diabetes mellitus is one of the most common endocrine diseases. Cardiovascular disease (CVD) is one of the leading causes of death in patients with type 2 diabetes. The aim of this study was to investigate the metabolic profile of plasma amino acids in diabetic patients with cardiovascular disease.
Methods: The present study is a descriptive-analytical cross-sectional study on 140 patients including 35 patients with type 2 diabetes and cardiovascular disease (CVD.DM), 35 patients with type 2 diabetes and non-cardiovascular disease (DM). 35 non-diabetic patients with cardiovascular disease (CVD.nDM) and 35 non-diabetic patients with non-cardiovascular disease (HS) were referred to Diabetes Clinic No. 1 of Tehran University of Medical Sciences.
Results: 76 (54.3%) were male and 64 (45.7%) were female. The highest concentrations of glutamine and isoleucine were observed in DM.CVD, asparagine, serine, arginine, threonine, alanine, tyrosine, valine in DM.nCVD and methionine in CVD.nDM. The lowest concentrations of tyrosine and tryptophan in DM.CVD has been detected , and methionine has been detected in DM.nCVD. The amino acids alanine, glutamine, tyrosine, valine, methionine, leucine, lysine and arginine significantly increased the chances of developing DM.nCVD. For each increase in Z-score per plasma concentration of isoleucine, the chances of developing cardiovascular disease without diabetes were significantly increased.
Conclusion: The amino acids alanine, glutamine, tyrosine, valine, methionine, leucine, lysine and arginine are involved in predicting the risk of DM.nCVD and isoleucine and methionine are involved in predicting the risk of CVD.nDM.
