Showing 2 results for Moderate Intensity Interval Training
Akbar Ghodratnama, Mahya Sharifi Rayeni, Arman Rastegari, Reza Moayedi,
Volume 24, Issue 1 (3-2024)
Abstract
Background: Cardiac function in diabetic people is affected through different cellular mechanisms; One of these important cellular pathways is the c-Jun N-terminal kinases (JNKs), whose dysfunction can lead to heart failure; Therefore, the aim of this research is to investigate the effect of moderate intensity interval training on total and phosphorylated content of c-Jun N-terminal kinases protein in heart tissue of type 1 diabetic rats.
Methods: In this experimental study, 12 two-month-old male Sprague Dawley rats with an average weight of 300±20 grams were selected. Type 1 diabetes (blood sugar above 300) was induced to rats through intraperitoneal injection of streptozotocin (with a dose of 50 mg per kilogram of body weight) solution. The rats were randomly divided into two groups, diabetic training and diabetic control; The training group performed MIIT for four weeks at an intensity of 55-75% of maximum speed. Data analysis was done through independent t-test in GraphPad Prism software version 9.5. The significance level of the current research is P≤ 0.05.
Results: The intracellular content of JNK protein after four weeks of MIIT showed a significant decrease in both total (P= 0.01) and phosphorylated (P= 0.0001) forms. On the other hand, the ratio of total to phosphorylated intracellular content of JNK protein did not show a significant change after four weeks of MIIT (P= 0.23).
Conclusion: MIIT led to a decrease in the content of total and phosphorylated forms of JNK protein, which could possibly lead to less cell death in cardiac cells of diabetic subjects.
Sajad Mirzaei, Hamed Alizadeh Pahlavani, Akbar Ghodratnama, Reza Moayedi,
Volume 24, Issue 5 (12-2023)
Abstract
Background: Adenosine monophosphate-activated kinase (AMPK) is a key regulator of cellular metabolism, and its dysregulation is associated with metabolic diseases such as obesity, inflammation, diabetes, and cancer. Therefore, the purpose of this research is the effect of moderate intensity interval training (MIIT) on the total and phosphorylated content of AMPKα1/2 protein in the skeletal muscle of diabetic rats.
Methods: In this experimental study, 12 two-month-old male Sprague Dawley rats with an average weight of 280±30 grams were selected. Diabetes was induced to rats through intraperitoneal injection of streptozotocin solution (with a dose of 65 mg per kg of body weight). These rats were randomly divided into two groups, diabetic training and diabetic control; The training group performed MIIT for 6 weeks at an intensity equal to 55-75% of maximum speed. Data analysis was done through independent t-test in GraphPad Prism version 10 software. The significance level of the current research is P≤0.05.
Results: Total intracellular content of AMPKα1/2 protein did not show significant changes in the training group compared to the control group in the soleus skeletal muscle (P=0.96). In contrast, the phosphorylated intracellular content (P=0.0001) and the ratio of phosphorylated to total form (P=0.002) of AMPKα1/2 protein showed a significant increase.
Conclusion: MIIT increased the protein content of AMPKα1/2 in soleus muscle tissue of diabetic rats, and this could lead to increased energy production and consumption and improved glucose levels in diabetic subjects.
