Showing 2 results for Nanocomposite
Zahra Mirzaeezadeh, Hajar Zarei, Kobra Omidfar,
Volume 13, Issue 3 (3-2014)
Abstract
Background: In this study, using anti-human serum albumin (HSA)-conjugated gold nanoparticles
(AuNPs) as an electrochemical label and mobile crystalline material-41 (MCM-41)–polyvinyl alcohol
(PVA) mesoporous nanocomposite as an immobilization platform, a new immunosensor was
established.
Methods: Field emission scanning electron microscopy (FESEM), cyclic voltammetry (CV), and
differential pulse voltammetry (DPV) have been applied to determine the physicochemical and
electrochemical properties of this hybrid film in immunosensor development.
Results: A suitable attachment between HSA and MCM-41 and also a thick layer deposition of
MCM-41–HSA–PVA film onto the electrode surfaces was observed by FESEM. DPV was employed
for quantitative determination of antigen.
Conclusion: In optimal conditions, this immunosensor could detect HSA in a high linear range (0.5–
200 µg ml
-1
with a low detection limit of 1 ng ml
-1
. This new strategy showed acceptable
reproducibility, stability, and reliability and could also be applied to detect the other antigens.
Kamyar Khoshnevisan, Sayed Mahmoud Sajjadi-Jazi, Farnoush Faridbod, Bagher Larijani, Mohammad Reza Khorramizadeh,
Volume 19, Issue 5 (6-2020)
Abstract
Background: A novel nanocomposite-modified electrode based on reduced graphene oxide (rGO) decorated with crown-ether and gold nanoparticles (GNPs) on the surface of a glassy carbon electrode (GCE) was fabricated to investigate 5-HT determination.
Methods: The morphology of nanocomposite was characterized by scaning electron microscopy (SEM). Diabetic zebrafish was obtained by overfeeding via glucose. 5-HT was successfully determined in the presence of dopamine (DA), ascorbic acid (AA), urea, glucose, and L-tryptophan (L-Trp) by using electrochemical methods.
Results: The nanocomposite exhibited satisfactory electrochemical catalytic activity for 5-HT determination using square-wave voltammetry (SWV). The electrochemical behavior of 5-HT at the nanocomposite/GCE displayed reasonable oxidation current and potential. The limit of detection (LOD) of 5-HT obtained from the real samples, containing the control and diabetic group by using the proposed system and HPLC method, was calculated to be about 0.3 and 0.1 µg/L, respectively. The prposed system also demonstrated high selectivity, reasonable sensitivity, and good stability and reproducibility for 5-HT sensing. The nanocomposite was applied for the determination of the biomarker 5-HT in the diabetic and control groups of zebrafish and displayed excellent recoveries about 93 and low relative error about 3% while compared with standard method.
Conclusions: It seems that the 5-HT level can be used for earlier diagnosis of diabetes.
