Showing 7 results for Nitric Oxide
Masoomeh Kourosh Arami, Abdolrahman Sarihi, Jila Behzadi, Seyed Mansour Malakouti, Iraj Amiri, Rafat Zare Ekbatani,
Volume 4, Issue 3 (5-2005)
Abstract
Background: The role of nucleus tractus solitarius in cardiovascular system regulation is controversial. On the other hand, study on the problem of hypertension in diabetic animals is the subject of many research programs. The aim of the present study was to determine wheather inactivation of nucleus can affect blood pressure in diabetic rats.
Methods: To this end , stereptosotocin-induced diabetic rats were anesthetized with Urethane and a cannula was inserted above nucleus. Blood pressure and heart rate were monitored using an intraarterial cannula. The cannulas were filled with L-glutamate (78 pmol/60 nL, to functionally identify the NTS see below), L-NAME(1nmol, to inhibit the nitric oxidergic neurons) and sodium nitroprusside (100mmol,as a NO-donor) .
Results: The results indicated that inactivation of nucleus in diabetic rats, had no effect on systolic and mean arterial pressure but enhanced diastolic blood pressure (P<0.05). There was no significant difference in heart rate between control and test groups.
Conclusion: Glucose affect on increasing blood pressure in rats with induced diabetes, in part, is caused by nitric oxidergic neurons resided in neucleus tractus solitarius.
Ali Mohammad Sharifi, Safar Mohseni, Sepideh Nekooparvar, Bagher Larijani, Hosein Fakhrzadeh, Shahrbanoo Oryan,
Volume 6, Issue 1 (8-2006)
Abstract
Background: Overweight is one of the most worldwide health problems particularly in industrial and developed communities. Obese subjects are at high risk for developing various disorders such as diabetes and especially cardiovascular diseases. It has been well established that life style modification plays an important role in reducing these complications, particularly weight reduction and caloric restriction (CR) as a non- pharmacological approach. In the present study the possible effects of caloric restriction on Nitric Oxide production and blood pressures in rat were investigated.
Methods: Four groups of rats were selected as control(C), caloric restriction (CR), diabetic (D), and caloric restriction diabetic (CRD). Control animals fed freely with standard pellet but caloric restricted animals fed an every other day diet for 4 weeks. The systolic blood pressure (SBP) was measured in all groups using the tail-cuff method under the light general anesthesia induced by ether. Nitric oxide (NO x) concentrations were determined in serum using a colorimetric non- enzymatic NO assay kit .
Results: In CR experimental groups (CR&CRD) SBP was significantly decreased as compared to control: (P<0.001) and diabetic (P<0.01) rats , while the serum NO x was significantly increased (P<0.01) in both groups as compared to control and diabetic rats.
Conclusion: On the basis of obtained results, it could be concluded that caloric restriction may reduce blood pressure and increase nitric oxide in diabetic and non-diabetic rats. Increase nitric oxide is the likely mechanism for decreasing blood pressure .
Ali Mohammad Sharifi, Maryam Ghaderpanahi, Seyed Ziaedin Hosseini Mazhari,
Volume 6, Issue 3 (5-2007)
Abstract
Background: Cardiovascular disease including hypertension are complications of long-standing diabetes. A few Studies had shown the positive effects of L-carnitine on hypertension. In this study, the possible effects of L-carnitne on nitric oxide (NO) levels and angiotensin-converting enzyme (ACE) activity in serum as well as systolic blood pressure (SBP) in diabetic and normal rats were studied.
Methods: In this study forty rats were used in four groups including non-treated control (C), L-carnitne treated control (CT), diabetic (D) and L-carnitne treated diabetic (DT). Diabetes was induced in rats by injection of stereptozotosin. Both of C & D groups had a free access to food and water and CT & DT groups were received daily dose of L-carnation in drinking water. At the end of 12 weeks SBP, serum NO and ACE activity were measured.
Results: Systolic blood pressure was significantly decreased in DT group compared to D group. Serum ACE activity was also significantly decreased in DT group compared to D group and the serum NO levels were significantly increased in DT & CT groups compared to D & C groups respectively.
Conclusion: Finally it could be concluded that L-carnitine may reduce SBP in diabetic rats via elevation of serum NO levels and reduction of serum ACE activity.
Mehrnoosh Mahrou, Abbas Ali Gaeini, Sirous Chobbineh, Mohsen Javidi,
Volume 14, Issue 1 (1-2015)
Abstract
Background: Exercise is very important factor in control of diseases. It also has been suggested that angiogenesis inhibitor factor is controlled and angiogenesis simulative factor is intensified on those who are suffering diabetic and doing physical activity. This study is aimed to evaluate the effect of Eight-week Resistance Training on unbalanced Angiogenesis in Diabetic male rats.
Methods: Twenty-four diabetic male Wistar rats were divided into two groups of control and training. Resistance training protocol includes one set of 10 times per day climbing the ladder suffering a weight connected to each rat tail [with respect to the maximum volume carrying each rat] for 3days a week and for 8 weeks. After 48 hour of the last training session, blood samples were taken from rat's hearts and VEGF, NO, glucose and insulin were determined regarding to serum sample taken. Analytical statistics examined with the use of SPSS16 software and considering α< 0.05.
Results: this study of Eight-week Resistance Training resulted no significant increase on VEGF [P=0.776] and NO [p=0.946] in diabetic rats serum but there was a significant decrease in blood glucose [p=0.001] however, no significant difference was observed in insulin level between the groups [p=0.93].
Conclusion: Despite resistance training appears to improve glucose levels in diabetic rats it has no positive effect on the stimulation factors of angiogenesis.
Kambiz Moradi, Masoud Jokar,
Volume 19, Issue 6 (8-2020)
Abstract
Background: The purpose of this study was to evaluate the effect of six weeks of endurance training with sour lemon consumption on plasma levels of endolin-1 and nitric oxide in streptozotocin-induced diabetic rats.
Methods: In this experimental study, 72 male Wistar rats (Weight; 200 ± 12 g) were selected and randomly divided into eight groups after becoming diabetic. Endurance training protocol was performed on rats for 6 weeks. Lemon essential oil (50 mg/kg) was administered using gavage. Plasma endolin-1 levels were measured by ELISA and serum nitrite levels were measured as a major metabolite of nitric oxide. One-way analysis of variance and Tukey's post hoc test were used for statistical analysis.
Results: The results showed that the mean levels of endothelin-1 in the endurance training + lemon group and diabetes + endurance training + lemon were significantly lower than the control group (p<0.05). Also, the mean nitric oxide levels in the diabetes + lemon group were significantly lower compared to the control group, but in the endurance + lemon training group and the diabetes + endurance training + lemon group was significantly higher than the control group (p<0.05).
Conclusion: According to the results of this study, it can be concluded that endurance training with consumption of lemon may improve endothelial function and vascular occlusion in diabetic patients by reducing the concentration of endothelin-1 levels and increasing nitric oxide levels.
Manijeh Namjoo, Parvaneh Nazarali, Rahman Soori, Azam Ramezankhani, Fatemeh Fallah,
Volume 22, Issue 4 (11-2022)
Abstract
Background: Adrenomodoline (ADM) is a multifunctional peptide involved in a wide range of physiological processes and cardiovascular function. The purpose of the present study was to compare the effect of physical activity on ADM and nitric oxide (NO) levels and insulin resistance in athletes and non-athletes.
Material and
Methods: 12 athletes and 11 non-athletes participated in this quasi-experimental study. Study participants were selected by convenience sampling. Both groups performed the Bruce protocol. Blood samples were collected before and after the training session to study the variables. Data were analyzed using one-way analysis of covariance, paired t-test, independent t-test and Pearson correlation.
Results: The results showed that after one session of submaximal exercise activity, HOMA-IR index decreased in non-athlete group (P = 0.034) and NO values increased in both athlete and non-athlete groups (P = 0.001, P = 0.002) and this increase was greater in the athlete group. Also, serum ADM levels were increased in the non-athlete group (P = 0.001) after the training session.
Conclusion: The single session of physical activity with increasing ADM and NO levels, can lead to better vasodilation and improved endothelial function as well as improved insulin resistance in inactive individuals. Since in athletes, only NO levels increased after the end of the activity, it seems that PA has a greater effect on improving the endothelial function of inactive people.
Maedeh Emamjomeh, Majid Hassanpourezatti, Atefeh Hosseinali,
Volume 22, Issue 6 (3-2023)
Abstract
Background: In this study, the effect of oral administration of recombinant human interleukin-2 (rhIL-2) on brain NO level and AChE activity in hyperglycemic conditions induced by high-sucrose diet (HSD), as a type-2 diabetes model, was investigated in Drosophila melanogaster.
Methods: In this experimental research, adult fruit flies of both sexes (30 in each group) were divided into the six groups: receiving normal diet (ND); high-sucrose diet (HSD); ND with rhIL-2 at 0.01 and 0.1 ng/ml; and HSD with rhIL-2 at 0.01 and 0.1 ng/ml. Flies were bred on these culture media for three weeks. At the end of the experiments, the brains of the flies were extracted, homogenized, and glucose, NO, and AChE activity levels were measured by the kit.
Results: Glucose level, AChE activity and NO level increased in brain homogenate of HFD flies compared to ND group. The body weight of HSD flies was reduced compared to the ND. Administration of rhIL-2 along with HFD significantly prevented these changes.
Conclusion: These findings suggest that rhIL-2 partially prevents diabetic neuropathy in Drosophila. It seems that the preventive effects of this compound are mediated through mechanisms dependent on nitric oxide and acetylcholinesterase in the brain.
