Showing 2 results for Nrf2
Moazameh Malekpour, Rasoul Rezai, Mohsen Salesi, Farhad Daryanoosh, Javad Nemati,
Volume 23, Issue 2 (5-2023)
Abstract
Background: Diabetes leads to numerous side effects in the cardiovascular system and also to improper functioning of the body's antioxidant system. The aim of this study is to investigate the effect of six weeks of high-intensity interval training with coenzyme Q10 supplementation on the values of Nrf2 and NQO1 in heart muscle of elderly diabetic rats.
Methods: In this experimental study, 48 elderly male rats (18 months old) were randomly divided into four groups of intense aerobic exercise with coenzyme Q10 supplementation, intense aerobic exercise group, coenzyme Q10 supplement intake group, and control group. Induction of diabetes was done by injecting a single dose of streptozotocin in the amount of 60 mg/kg intraperitoneally. The program of high intensity interval training was done for six weeks. Coenzyme Q10 supplement group received orally at a dose of 200 mg/kg. Western blot method was used to measure Nrf2 and NQO1 values. The data were analyzed by one-way analysis of variance and Tukey's post hoc test at a significance level of P <0.05.
Results: The results showed taking coenzyme Q10 supplement (P= 0.014), intense intermittent exercise (P= 0.001) and intense intermittent exercise with coenzyme Q10 supplement (P= 0.001) significantly increased Nrf2 values in the hearts of diabetic elderly rats. Also, the use of coenzyme Q10 supplement (P=0.0366), intense intermittent exercise (P= 0.014) and intense intermittent exercise along with coenzyme Q10 supplement use (P= 0.002) significantly increased the amount of NQO1 in the hearts of diabetic aged male rats.
Conclusion: Intermittent intense training along with supplement consumption improves heart function in elderly diabetic patients through increasing endogenous antioxidant enzymes.
Yeganeh Golmohammadi Samani, Parvaneh Nazarali, Rostam Alizadeh, Najmeh Rezaeinezhad,
Volume 24, Issue 4 (10-2024)
Abstract
Background: Aging and consuming a high-fat diet lead to increased oxidative damage to various tissues, oxidative stress is a critical factor in the aging process that can cause direct damage to cellular structure. This study aimed to investigate the Effects of 8 Weeks of HIIT Training on the Nrf2 Gene Expression, lipid peroxidation and Insulin resistance in the pancreas tissue of Aged rats fed a high-fat diet.
Methods: In this experimental study, 20 aged male Wistar rats (age: 18 months and mean weight: 500±100 gr) were randomly divided into four groups including normal food control G1 (n=5), normal food + training G2 (n=5), high-fat food G3 (n=5) and high-fat food +training G4 (n=5). The high intensity interval training program was performed on a treadmill, three days a week for eight weeks. Nrf2 gene expression was performed using real-time PCR and malondialdehyde levels, glucose and insulin were measured using a kit and ELISA method. Data were analyzed by MANOVA test at the P<0.05.
Results: The results of the MANOVA statistical test on the interactive effect of training and diet indicated a significant difference in the insulin resistance index (P = 0.017 and F = 7.17). However, no significant effect was observed for the insulin factor (P = 0.30 and F = 1.13), glucose (P = 0.116 and F = 2.75), MDA (P = 0.87 and F = 0.028), and Nrf2 (P = 0.816 and F = 0.056).
Conclusion: In general, it can be stated that HIIT training in this research can improve insulin resistance by affecting the expression of the Nrf2 transcription factor gene by reducing the oxidant activity in aged rats.
