Malihe Aveseh, Rohollah Nikooie, Fereshteh Atabi, Zahra Mirzaie Zadeh , Kobra Omidfar, Bagher Larijani,
Volume 13, Issue 2 (1-2014)
Abstract
Background: the aim of this study was to investigate the effect of seven weeks endurance training on
RBP4 gene expression in Soleus and Extensor Digitrum Longouse (EDL) Muscles, liver, visceral and
subcutaneous fat in type 2 diabetic rats.
Methods: 50 male wistar rats (5 weeks years old, weight = 93.7 ± 8.9) were purchased and randomly
divided into four groups: Control (n=10) (C), Trained (n=10) (T), Diabetic Control (n=15) (DC) and
Trained diabetic (n=15) (TD). Diabetes was induced by injection of low dose of streptozotocin (STZ)
and feeding with high fat diet. Insulin resistance accuracy was confirmed by HOMA-IR index and
Real-time PCR was used for mRNA content.
Results: After seven weeks of diabetes induction, the RBP4 mRNA content of the liver (2.37-fold P <
0.01), visceral fat (2.33-fold P < 0.01), and subcutaneous fat (1.83-fold P < 0.05), soleus (1.21-fold
P < 0.05) and EDL (2.03-fold P < 0.05) were increased. After seven weeks of endurance training
significant decrease in RBP4 mRNA content was found in visceral fat (P < 0.05), subcutaneous fat (P
< 0.05) and EDL (P < 0.05) between DC and CD. In addition, significant difference between T and TD
groups was found for RBP4 mRNA content in liver (p < 0.01), subcutaneous fat (P < 0.01) and EDL
(P < 0.01) after seven weeks of endurance training.
Conclusion: Type 2 diabetes considerably increases skeletal muscle RBP4 expression in isoform-
specific manner. This increase is also seen in liver, subcutaneous and visceral fat. In addition,
endurance training decreases the RBP4 expression in EDL, subcutaneous and visceral fat.
