Showing 4 results for Royal Jelly
Mahdieh Ghasemi, Ahmad Abdi, Asieh Abbassi Daloii,
Volume 22, Issue 5 (12-2022)
Abstract
Background: Inflammation plays a critical role in the promotion of hepatocyte damage and liver fibrosis. In recent years the protective role of exercise and royal jelly (RJ), has been suggested for metabolic and inflammatory disorders. In this study, we aimed to evaluate the effects of aerobic training (AT) and RJ on pro-inflammatory markers in a rat's model of a high-fat diet (HFD).
Methods: In this experimental study, 45 male rats were randomly divided into five groups (Each group: n=9): Normal Diet (ND), High-Fat Diet (HFD), High-Fat Diet +Training (HFDT), High-Fat Diet + Royal Jelly (HFDRJ), and High-Fat Diet +Training + Royal Jelly (HFDTRJ). The supplement groups received 100 mg of royal jelly (kg/body weight) diluted in distilled water orally during the intervention period. The aerobic exercise program included treadmill running with an intensity of 50-60% oxygen consumption (VO2max), 5 days/week for 8 weeks.
Results: HFD increased hepatic NF-κB (p=0.006), TNF-α (p=0.0001) and IL-1β (p=0.0001). AT, RJ as well as the combination of AT with RJ decreased NF-κB, TNF-α and IL-1β (p≤0.05). Decrease in NF-κB, TNF-α and IL-1β were significant in HFDTRJ group compared to HFDT and HFDRJ groups (p≤0.05).
Conclusion: The combination of AT and RJ may decrease inflammatory markers and improve HFD-induced hepatic steatosis by reducing the expression of NF-κB, TNF-α, and IL-1β.
Masuad Jahantash, Hossein Abednatanzi, Mandana Gholami, Farshad Ghazalian,
Volume 22, Issue 6 (3-2023)
Abstract
Background: Type 2 diabetes is the most common endocrine disease that can cause tissue damage and apoptosis. The purpose of the present study was to study the changes in the expression of related liver apoptosis genes after High Intensity Training (HIT) and royal jelly in type 2 diabetic rats.
Methods: The statistical sample of the study was 36 male rats that became diabetic after 20 weeks of high-fat diet and injection of 25 ml/kg of STZ. Fasting glucose between 150 and 400 mg/dl was considered as the criteria for type 2 diabetes. Diabetic rats were placed in 4 groups: control, HIIT, Jelly, and HIIT-Jelly. The HIITprotocol was performed 8 weeks, 5 sessions / week with intense 2-minute intervals with 2-8 intervals and 80-90% vo2max and one-minute rest intervals with 50-56% vo2max and Royal Jelly gavage at 100 mg/kg for 5 days / week. Data analysis was done using one-way and two-factor analysis of variance and post hoc test.
Results: The results showed that compared to the control group, HIIT led to a significant decrease in glucose and insulin resistance index. Also, HIIT and royal jelly consumption led to a decrease in Bax gene expression and an increase in Bcl2 gen expression and a decrease in the Bax/Bcl2 ratio in liver cells compared to the control group (P<0.05).
Conclusion: HIIT with royal jelly in diabetic rats led to the reduction and improvement of glycemic index and insulin resistance and appropriate changes in the expression of liver apoptotic genes.
Sahar Riasati, Hossein Abednatanzi, Mandana Gholami,
Volume 24, Issue 1 (3-2024)
Abstract
Background: The Purpose of this study was to evaluate the effect of High Intensity Interval Training & Royal Jelly on expression of the genes of the hepatic miR-423-5P - FAM3A-AKt2 pathway in rats.
Methods: The statistical population was 36 male Wistar rats and reaching a weight of 193 ± 20 grams, they were subjected to a high-fat diet diet and intraperitoneal injection of 25 mg STZ. Blood sugar above 150 to 400 mg/dl was considered as a criterion to ensure that the mice had type 2 diabetes. Then divided into 4 groups (diabetic control group, intermittent exercise, Royal Jelly and exercise-Royal Jelly group). The experimental group of did HIIT with a 20 to 36 m/min. for 8 weeks. Royal jelly experimental groups consumed 100 mg/kg royal Jelly. 48 hours after the last training session, the mice were anesthetized with ether and blood was taken from the heart and tissue was removed, including liver tissue, and the tissue sample was transferred to a freezer temperature of -80. Serum glucose measurements were performed by an Auto analyzer. Insulin was measured by an ELISA kit, and the insulin resistance index was measured by formulas. The expression level of miRNA-423-5P, FAM3A and AKt genes in liver tissue was measured using Real Time-PCR method .the data, analyzed by one-way analysis of variance and Bonferroni’s post hoc and univariate and effect size. The significance level was considered to be p≥ 0.05.
Results: The findings showed that the expression of the hepatic miR-423-5P gene in the experimental groups of intermittent exercise with royal jelly and the exercise group and the gel group significantly decreased compared to the control group, and the expression of the FAM3A gene increased in the experimental groups compared to the control group. Non-significant and the expression of AKt2 gene in the training group and the training group with gel increased significantly compared to the control group, and in the gel group only compared to the control group, there was a non-significant increase, Also, there was a significant decrease in glucose in the experimental groups of HIIT exercise and HIIT- Royal jelly compared to the diabetic control group (p <0.05).Expression of the miR-423-5P gene was significantly lower in all groups compared to control, expression of mRNA FAM3 had insignificant increase and expression of mRNA AKt2 had a significant increase in groups of regular exercise and exercise with nutritional intervention. This gene had an insignificant increase of expression in the group with only nutritional intervention.
Conclusion: It seems that HIIT and royal jelly consumption, both alone and in combination, although with greater effect, can create favorable changes in the expression of Mir 4235p. FAM3A and AKt2 help to improve the condition of the liver.
Mrs Fateme Saebi, Hossein Abednatanzi, Mohamad Ali Azarbayejani, Mrs Mandana Gholami,
Volume 24, Issue 5 (12-2023)
Abstract
The aim of the present study was to study the changes in GLUT4 gene expression in soleus muscle tissue and insulin resistance index after HIIT and royal jelly in type 2 diabetic obese rats. Methods: The statistical sample of the present study was 36 obese diabetic male rats. After 20 weeks of feeding with a high-fat diet, the rats became diabetic by intraperitoneal injection of 25 mg/kg of STZ. Diabetic rats were grouped into 4 groups: control, intermittent exercise, royal Jelly, HIIT-Jelly. Eight weeks HIIT, five sessions per week with 2-minute HIIT of 80 to 90% and one-minute rest interval were performed. Royal jelly was given by gavage with a dose of 100 mg/kg before training. Findings: Data analysis using one-way and two-way analysis of variance and post-hoc test showed that compared to the control group, HIIT led to a significant decrease in glucose and insulin resistance index HIIT and royal jelly led to a significant increase in GLUT4 gene expression in soleus muscle compared to the control group (P=0.001). Conclusion: HIIT and royal jelly were effective in reducing insulin resistance index and expression of genes effective in glucose consumption in soleus muscle. Also, HIIT and royal jelly led to an increase in GLUT4 gene expression in the soleus muscle compared to the control group, which is important in glucose consumption in diabetics.
