Seyedeh Neda Mousavi, Sara Gharacheh, Mir Saeed Seyed Dorraji, Elham Hosseini, Fariba Koohdani,
Volume 23, Issue 1 (5-2023)
Abstract
Background: Animal studies have shown maternal low/ high-calorie diet during pregnancy can alter metabolism of offspring through change in Sirtuin 1 (SIRT1) expression, as a metabolic sensor. However, there is no study on the effects of isocalorie diet. Herein, effects of two isocalorie diets with different amounts of fat were assessed on SIRT1 gene and protein level in the liver of male and female offspring.
Methods: The control group received AIN93G diet. In this diet, 16% and 64% of total calorie were prepared from fat and carbohydrate, respectively. The intervention group received high fat AIN93G diet contained 48% and 32% of calorie from fat and carbohydrate, respectively. In both diets, fat was prepared from soy oil. Diets were isocaloric and 20% of total calorie was provided from protein. Mothers categorized to one of these diets and offspring received the control diet after weaning (3 weeks after the birth).
Results: SIRT1 gene and protein levels were lower in male and female offspring born from mothers received high-fat diet than the controls (p<0.001).
Conclusion: Change in maternal dietary fat, without increase in calorie, effects on gene and protein levels of SIRT-1 in the liver of next generation.
Reza Amini, Abbas Sarmi, Mohammad Reza Afarinesh ,
Volume 24, Issue 5 (12-2024)
Abstract
Background: Alzheimer's disease is the most common type of dementia, which is associated with progressive loss of memory and reaction to the environment. The aim of this study was to investigate the effect of 12 weeks of resistance training with sumac intake on the serum levels of SIR3, SIR4 and AMPK in Alzheimer's male rats.
Methods: In this experimental study, male Wistar rats with an age of 8-10 weeks and an average weight of 230±30 grams were randomly divided into five groups: control, Alzheimer's disease, Alzheimer's disease with sumac supplementation, Alzheimer's disease with resistance training, and Alzheimer's disease with resistance training. Received sumac supplements were divided. To induce Alzheimer's disease, 8 mg/kg of trimethyl tin chloride along with 200 microliters of normal saline were injected intraperitoneally to mice. Resistance training was performed for 12 weeks and 5 days a week on a special rat ladder. 48 hours after the last training session, a blood sample was taken. Serum levels of SIR3, SIR4 and AMPK were evaluated by ELISA method. To check the changes between the control group and other groups using the independent T-test and to check the difference between the other groups using the one-way analysis of variance test and if significant, using the Tukey post hoc test, by SPSS version 29 software at a significance level of P< 0.05 used.
Results: The results of this research showed that 12 weeks of resistance training increases the levels of SIR3 (P< 0.036), SIR4 (P< 0.029) and AMPK (P< 0.447) compared to the Alzheimer group. In addition, adding sumac to the resistance training program had a significant effect on the levels of SIR3 (P< 0.078), SIR4 (P< 0.024) and AMPK (P< 0.002).
Conclusion: Our results suggest that resistance training is associated with the improvement of sirtuin 3, sirtuin 4 and adenosine monophosphate kinase levels in Alzheimer's rats and receiving sumac antioxidant compound leads to doubling of these responses.
