Showing 6 results for Soleus Muscle
Azam Karami Paskohani, Masoud Rahmati , Abdolreza Kazemi ,
Volume 14, Issue 3 (3-2015)
Abstract
Background: Diabetic neuropathy leads to skeletal muscle atrophy however atrophy signaling mechanisms are not well documented. The aim of the present study was to investigate Sunday Driver (Syd) gene expression in soleus muscle of Wistar male rats with diabetic neuropathy. Methods: Twelve male Wistar rats were randomly assigned in 3 groups: diabetic trained, diabetic untrained and healthy control. Two weeks after STZ injection (45 mg/Kg), diabetic neuropathy was demonstrated with mechanical allodynia and thermal hyperalgesia tests and after which moderate endurance training protocol was performed for 6 weeks. 48 hours after final training session, the rats were dissected and soleus muscle tissues were removed. Also Sydgene expression was measured with Real time- PCR methods. Results: Soleus muscle weight was decreased in diabetic groups (P=0.001), but compared with diabetic untrained group, was higher in diabetic trained group (P=0.001). Sydgene expression in diabetic untrained group was higher than healthy control group (P=0.001). Also, compared with diabetic untrained group, training significantly decreased Sydgene expression and blood glucose levels in diabetic trained group. (P=0.001 and P=0.0001, respectively). Conclusion: In soleus muscle of diabetic rats, Sydm RNAup-regulation is involved in development of muscle atrophy and training as a non-pharmacotherapy strategy can modulate and get it close to normal levels. So, it is suggested that Syd should be noted as a novel treatment in diabetes disease.
Masoud Rahmati, Abdolreza Kazemi, Mohammad Hosein Archangi, S.jalal Taherabadi,
Volume 14, Issue 6 (9-2015)
Abstract
Background: Neuropathic pain is associated with a profound impact on quality of life and daily activity in patients and caused high medical costs. Because of the nerves destructive nature in this disease, the neurotrophins are high regarded for their possible effects on survival, growth and development and neuronal functions as a possible therapeutic strategy. So the aim of this study is investigation of the chronic effects of decreased activity in neuropathic pain form on the NFG gene expression in the sciatic nerve of Spinal nerve ligated rats.
Methods: Ten adult male Wistar rats in the weight range of 250±30 gr randomly were divided into two groups including healthy control (C), ligation sciatic nerve ligation. Over the six weeks neuropathic pain behavior tests were conducted continually in groups. In the end change of NGF gene expression in sciatic nerve was measured with Real time PCR technique and calculated using the 2-&Delta&DeltaCT method.
Results: After 6 weeks, neuropathic pain behavior tests showed that pain threshold of thermal hyperalgesia and mechanical allodynia in the SNL group was significantly lower than the control group (P&le0.05). In addition, NGF gene expression in sciatic nerve ligation group compared to controls increased significantly (P&le0.05).
Conclusion: The findings of this study suggest that the development of neuropathic pain and decreased physical activity is associated with increased endogenous NGF. Although it is not clear that increase in NGF expression is due to the nature of neuropathic pain or nerve damage, it is clear that excessive expression of NGF is associated with neuropathic pain.
Maryam Taheri, Mehrzad Ebadi Ghahramani, Farah Nameni,
Volume 23, Issue 5 (12-2023)
Abstract
Background: Sirtuin-1 is known as a key metabolic sensor in various tissues, including skeletal muscle and tissue, which can play a pivotal role in regulating glucose and lipid metabolism through diacetylase activity. The aim of the present study was to investigate the role of licorice extract and swimming exercise on SIRT1 signaling pathways in the soleus muscle of diabetes male rats.
Methods: The research is of an experimental and fundamental type, and the working method is an experimental work with a descriptive-solution nature. Fifty mice were bought and divided into 5 groups of 10. The main training program consisted of swimming in the rodent tank of the special swimming water tank for 6 weeks and 5 sessions per week. In order to induce diabetes in the mice, streptozotocin was injected. Seven days after the injection, the blood sugar of the mice was measured, and the mice with blood sugar more than 250 ml/dL were selected as diabetes samples. Licorice root extract was given to two groups of rats. In order to analyze, two-way analysis of variance was used using SPSS26 software.
Results: The results showed that the interaction effect of swimming training and consumption of licorice extract on SIRT1 signaling pathways is statistically significant (P= 0.002).
Conclusion: SIRT1 plays an important role in the control of glucose homeostasis. In fact, under certain conditions, the activity and expression level of SIRT1 gene decreases in different SI tissues due to its important metabolic role. Swimming training along with licorice supplement may be used for prevention and treatment of diabetes by positive regulation of SIRT1 gene expression.
Mahya Sharifi Rayeni, Farhad Daryanoosh, Mohsen Salesi, Maryam Kooshki Jahromi,
Volume 24, Issue 2 (6-2024)
Abstract
Background: Mitophagy is a type of cell death that regulates the quality of mitochondria and can lead to disorders in diseases such as diabetes. Therefore, the purpose of this study was to investigate the effect of high-intensity interval training (HIIT) on the content of proteins related to the mitophagy pathway (LC3 and BNIP3L) in muscle tissue soleus of rats with type 2 diabetes.
Methods: In this experimental study, 18 three-month-old male Sprague Dawley rats with an average weight of 270±30 g were selected. Rats were infected with type 2 diabetes by intraperitoneal injection of a streptozotocin and nicotinamide solution. Rats were randomly divided into two groups: diabetic and diabetic. A healthy control group was also included. The training group performed HIIT for eight weeks at an intensity of 85-95% of the maximum speed. Data analysis was performed using a one-way ANOVA test in GraphPad Prism version 9.5 software. A significance level of P≤ 0.05 was considered statistically significant.
Results: The levels of LC3 and BNIP3L proteins significantly increase after eight weeks of HIIT compared to both the diabetic and healthy control groups (P= 0.0001).
Conclusion: It can be concluded that HIIT by increasing the factors related to mitophagy can cause the cleaning of dysfunctional mitochondria in the muscle of diabetic subjects; However, excessive mitophagy can also cause functional defects in regulating the quality of mitochondria.
Sajad Mirzaei, Hamed Alizadeh Pahlavani, Akbar Ghodratnama, Reza Moayedi,
Volume 24, Issue 5 (12-2024)
Abstract
Background: Adenosine monophosphate-activated kinase (AMPK) is a key regulator of cellular metabolism, and its dysregulation is associated with metabolic diseases such as obesity, inflammation, diabetes, and cancer. Therefore, the purpose of this research is the effect of moderate intensity interval training (MIIT) on the total and phosphorylated content of AMPKα1/2 protein in the skeletal muscle of diabetic rats.
Methods: In this experimental study, 12 two-month-old male Sprague Dawley rats with an average weight of 280±30 grams were selected. Diabetes was induced to rats through intraperitoneal injection of streptozotocin solution (with a dose of 65 mg per kg of body weight). These rats were randomly divided into two groups, diabetic training and diabetic control; The training group performed MIIT for 6 weeks at an intensity equal to 55-75% of maximum speed. Data analysis was done through independent t-test in GraphPad Prism version 10 software. The significance level of the current research is P≤ 0.05.
Results: Total intracellular content of AMPKα1/2 protein did not show significant changes in the training group compared to the control group in the soleus skeletal muscle (P= 0.96). In contrast, the phosphorylated intracellular content (P= 0.0001) and the ratio of phosphorylated to total form (P= 0.002) of AMPKα1/2 protein showed a significant increase.
Conclusion: MIIT increased the protein content of AMPKα1/2 in soleus muscle tissue of diabetic rats, and this could lead to increased energy production and consumption and improved glucose levels in diabetic subjects.
Keywords: Adenosine Monophosphate-activated Kinase (AMPKα1/2), Moderate Intensity Interval Training, Soleus Muscle, Diabetes
Mrs Fateme Saebi, Hossein Abednatanzi, Mohamad Ali Azarbayejani, Mrs Mandana Gholami,
Volume 24, Issue 6 (2-2024)
Abstract
The aim of the present study was to study the changes in GLUT4 gene expression in soleus muscle tissue and insulin resistance index after HIIT and royal jelly in type 2 diabetic obese rats. Methods: The statistical sample of the present study was 36 obese diabetic male rats. After 20 weeks of feeding with a high-fat diet, the rats became diabetic by intraperitoneal injection of 25 mg/kg of STZ. Diabetic rats were grouped into 4 groups: control, intermittent exercise, royal Jelly, HIIT-Jelly. Eight weeks HIIT, five sessions per week with 2-minute HIIT of 80 to 90% and one-minute rest interval were performed. Royal jelly was given by gavage with a dose of 100 mg/kg before training. Findings: Data analysis using one-way and two-way analysis of variance and post-hoc test showed that compared to the control group, HIIT led to a significant decrease in glucose and insulin resistance index HIIT and royal jelly led to a significant increase in GLUT4 gene expression in soleus muscle compared to the control group (P=0.001). Conclusion: HIIT and royal jelly were effective in reducing insulin resistance index and expression of genes effective in glucose consumption in soleus muscle. Also, HIIT and royal jelly led to an increase in GLUT4 gene expression in the soleus muscle compared to the control group, which is important in glucose consumption in diabetics.
