Showing 6 results for Stem Cells
Leila Eini, Negar Naghash, Bagher Larijani, Jafar Ai, Keivan Majidzadeh, Esmaeil Sadroddiny, Kobra Omidfar,
Volume 13, Issue 2 (1-2014)
Abstract
Background: Mesenchymal stem cells (MSCs), also known as mesenchymal stromal cells, are
considered as an important source of adult stem cells in tissue engineering and cell therapy. They are
present in various tissues such as, endometrium as the supportive cells. According to anatomical
position of endometrial mesenchymal stem cells that put them in neighborhood of the fetus, they may
have a significant role in fetus tolerance during pregnancy.
This study was conducted to evaluate the molecular mechanism of immunosuppressive affect of
endometrial mesenchymal stem cells.
Methods: Mesenchymal Cells from bone marrow and endometrium were cultured at density of 2 ×105
cells/ml at presence of 100IU/ml and 500IU/ml INF-γ (IFN-gamma) and expression of indoleamine 2
3-dioxygenase (IDO) were studied after 72 hours by real-time PCR and immunocytochemistry.
Results: The study showed that IDO expression in cells exposed to IFN-γ was increased compared to
the cells in the absence of IFN-γ (p<0.05). Additionally, up regulation of IDO expression was higher
in endometrial cells than bone marrow cells.
Conclusion: From these results it is concluded that endometrial mesenchymal stem cells may be used
as a good candidate for cell therapy.
Akram Tayanloo-Beik, Hamidreza Aghayan, Sepideh Alavi-Moghadam, Mostafa Rezaei-Tavirani, Shayesteh Kokabi Hamidpour, Babak Arjmand, Bagher Larijani,
Volume 20, Issue 1 (1-2021)
Abstract
Background: In many years, finding modern and appropriate treatments for diseases, especially chronic diseases (such as chronic metabolic diseases) have been one of the most important issues. On the other hand, limitations related to organ transplantation have made a serious need for advanced therapeutic strategies. In this respect, researchers have recognized the benefits of stem cells as a suitable tool. Accordingly, the application of stem cells for the treatment of various diseases has been considered in many countries around the world especially Iran.
Methods: A review of the history of stem cell studies in Iran and the world was conducted and the Cell Therapy and Reconstructive Medicine Research Center was introduced as one of the leading centers in this field and a review was conducted on the activities of this center.
Results: The history of studies related to cell-based therapy in Iran back to 1990. Subsequently, the Cell Therapy and Reconstructive Medicine Research Center, by providing services based on GMP principles, has provided clinical services in accordance with international standards and has conducted various researches in this field.
Conclusion: The purpose of the current review is to examine the strategies and challenges around the research and treatment opportunities using stem cells in mentioned center.
Syed Reza Mir Javadi, Alireza Rahimi, Fariba Aghaei, Mahsa Mohsenzadeh,
Volume 21, Issue 5 (1-2022)
Abstract
Background: Because insulin therapy cannot properly control the progression of diabetes and its complications, other alternative therapies may be desirable. The aim of this study was to investigate the effect of resistance training and endothelial stem cell injection on skeletal muscle oxidant and antioxidant status in STZ-induced diabetic male rats.
Method: In this experimental study, 36 male Wistar rats (age 6 weeks) were divided into six groups of control (healthy), basal diabetic control, diabetic control, diabetes + stem cell injection, diabetes + resistance training and diabetes + stem cell injection + resistance training. In this study, rats became diabetic intraperitoneally using streptozotocin as a single dose of 40 mg/kg. Resistance exercises including climbing a one-meter ladder with weights hanging from the tail were performed for 17 sessions. 500,000 bone-derived stem cells were injected by a cell counter. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in skeletal muscle tissue of rat were measured by using the kit and ELISA method.
Results: The results showed that the SOD level of rats in the resistance training and endothelial stem cell injection group was significantly higher than the diabetic control group (P<0.001). Also, the level of MDA rats in the resistance training and endothelial stem cell injection group was significantly lower than the control group (P<0.001).
Conclusion: Resistance training and endothelial stem cell injections can be considered as a non-pharmacological treatment to reduce skeletal muscle complications in type 1 diabetes.
Elham Mokhtari, Amir Sarshin, Foad Feizolahi, Eidi Alijani,
Volume 22, Issue 2 (7-2022)
Abstract
Background: Type 1 diabetes is associated with decreased skeletal muscle capillary and improper regulation of angiogenesis pathways in skeletal muscle. This research intended to study the effect of resistance training and endothelial stem cell injection on βeta-actin, phosphorylated and total AKT of skeletal muscle in type 1 diabetic rats.
Methods: In this experimental study, 36 male Wistar rats (age 6 weeks) were divided into six groups of control (healthy), basal diabetic control, diabetic control, diabetes + stem cell injection, diabetes + resistance training and diabetes + stem cell injection + resistance training. In this study, rats became diabetic intraperitoneally using streptozotocin as a single dose of 40 mg/kg. Resistance exercises including climbing a one-meter ladder with weights hanging from the tail were performed for 17 sessions. 500,000 bone-derived stem cells were injected by a cell counter. The levels of βeta-actin, phosphorylated and total AKT in skeletal muscle tissue of rat were measured by using the Western blotting method.
Results: The results showed that resistance training led to significant increase in Pho-AKT, β-actin and Pho-AKT/AKT ratio and significant decrease in AKT of muscle tissue in type 1 diabetic rats (P<0.001). Injection of stem cells leads to significant increase in Pho-AKT and Pho-AKT/AKT ratio and resistance training with simultaneous injection of stem cells leads to significant increase in Pho-AKT, β-actin and significant decrease in Akt of muscle tissue in type 1 diabetic rats (P<0.001).
Conclusion: According to the results, it is possible that the intervention of resistance training with injection of stem cells can help regulate the pathways of skeletal muscle angiogenesis in type 1 diabetes.
Masomeh Sarmadiyan, Eidy Aliijani, Fuad Feizolahi, Davood Khorshidi,
Volume 22, Issue 3 (9-2022)
Abstract
Background: Type 1 diabetes is a disorder caused by autoimmune destruction of pancreatic insulin-producing cells. This induction of autoimmunity may be due to genetic and environmental factors. Bax and Bcl2 proteins play an important role in the process of apoptosis.
Methods: In this study, 30 male Wistar rats weighting approximately 200±20gr were randomly selected from available rats in lab (500). Subjects after 2 weeks of familiarity with the environment were randomly divided into 5 groups of 6, including (diabetes + injection, exercise) and (diabetes + exercise) and (diabetes + injection) and (diabetes control to control the passage of time) and (basic diabetes to Defaults) under the same laboratory conditions and developed type 1 diabetes by intraperitoneal injection of streptozotocin (stz) (60 mg / kg). Rats in the diabetic group and the diabetic group + stem cell injection had a total of 17 sessions of resistance training for 5 weeks.
Results: The results of the present study showed that there was no significant difference between the mean of Bax and Bcl2 in the resistance training group with simultaneous injection of stem cells and the training group.
Conclusion: The results of our study showed that performing 17 sessions of resistance training alone with stem cell injection was effective on the expression of Bax protein and Bcl2, but there was no significant difference between the effect of training alone and training with stem cells.
Asma Soleimani, Mohammad Fathi, Mostafa Bahrami,
Volume 24, Issue 3 (7-2024)
Abstract
Background: The purpose of this study was to investigate the effect of sports rehabilitation exercises with vitamin D consumption on fatigue, sleep quality and depression level of people with osteosarcoma cancer after bone marrow stem cell transplantation.
Methods: In this study, 27 women with osteosarcoma cancer with body mass index above 30 kg/m2, age 24.90±4.03 years, height 163.85±3.62 cm and weight 82.17±4.5 kg were randomly divided into four groups. (patient-exercise), (patient-exercise-vitamin D), (patient-exercise-placebo) and (control) were divided. The training protocol includes ten weeks of Pilates exercise. The supplement group took 500 IU of vitamin D three days a week for 10 weeks. Data were analyzed using dependent t-test and one-way analysis of variance.
Results: The results of the covariance analysis of the research of three exercise groups showed that after ten weeks of sports rehabilitation with vitamin D consumption, the indicators of fatigue and the level of depression decreased significantly (P≤ 0.05). Also, the results of covariance analysis of the three training groups showed that the overall sleep quality of people with osteosarcoma cancer increased significantly (P≤ 0.05). Conclusion: Based on the obtained results, it is suggested that the consumption of vitamin D along with exercise rehabilitation can be an effective treatment method for fatigue, sleep quality and depression in people with osteosarcoma cancer.
Conclusion: Based on the obtained results, it is suggested that the consumption of vitamin D along with sports rehabilitation can be an effective treatment method for fatigue, sleep quality and depression in people with osteosarcoma cancer.
