Showing 2 results for Superoxide Dismutase
Eftekhar Mohammadi, Fatemeh Nikseresht,
Volume 19, Issue 5 (6-2020)
Abstract
Background: Diabetes and its oxidative stress increase the effects of this disease on heart tissue. On the other hand, exercise improves the antioxidant status of heart tissue. The aim of this study was to investigate the effect of 8 weeks of increased endurance training on superoxide dismutase activity and malondialdehyde levels in the heart tissue of mice with type 2 diabetes.
Methods: In this experimental study, 24 male Wistar rats (256 ±11.8 g, 10 weeks old) were divided into 4 groups of 6. Exercise program for 8 weeks of increasing endurance training. 48 h after completion of the protocol, the activity of superoxide dismutase enzyme and malondialdehyde levels in rat heart tissue were measured. One-way analysis of variance was used for group comparisons and Pearson test was used to examine the relationship between indicators.
Results: There were significant difference between the four groups in superoxide dismutase (P= 0.001) and malondialdehyde (P= 0.001) indices. As a result of post-hoc test, there were significant increase in superoxide dismutase index in healthy exercise (P= 0.016) and control groups (P= 0.029) compared to diabetic control group and significant decrease in malondialdehyde index in control (P= 0.003), diabetic exercise (P= 0.050) and healthy exercise groups (P= 0.001) compared to diabetic control group. Significant correlation was observed between superoxide dismutase and malondialdehyde indices (r= 0.018, P= 0.274).
Conclusion: According to the results of this study, it seems that incremental endurance training reduces lipid peroxidation and improves antioxidant status and consequently reduces oxidative stress in cardiac tissue of diabetic rats.
Syed Reza Mir Javadi, Alireza Rahimi, Fariba Aghaei, Mahsa Mohsenzadeh,
Volume 21, Issue 5 (1-2022)
Abstract
Background: Because insulin therapy cannot properly control the progression of diabetes and its complications, other alternative therapies may be desirable. The aim of this study was to investigate the effect of resistance training and endothelial stem cell injection on skeletal muscle oxidant and antioxidant status in STZ-induced diabetic male rats.
Method: In this experimental study, 36 male Wistar rats (age 6 weeks) were divided into six groups of control (healthy), basal diabetic control, diabetic control, diabetes + stem cell injection, diabetes + resistance training and diabetes + stem cell injection + resistance training. In this study, rats became diabetic intraperitoneally using streptozotocin as a single dose of 40 mg/kg. Resistance exercises including climbing a one-meter ladder with weights hanging from the tail were performed for 17 sessions. 500,000 bone-derived stem cells were injected by a cell counter. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in skeletal muscle tissue of rat were measured by using the kit and ELISA method.
Results: The results showed that the SOD level of rats in the resistance training and endothelial stem cell injection group was significantly higher than the diabetic control group (P<0.001). Also, the level of MDA rats in the resistance training and endothelial stem cell injection group was significantly lower than the control group (P<0.001).
Conclusion: Resistance training and endothelial stem cell injections can be considered as a non-pharmacological treatment to reduce skeletal muscle complications in type 1 diabetes.
