Showing 4 results for Vegf
Javad Tavakkoly Bazzaz, Vera Pravica, Andrew Jm Boulton, Ian V Hutchinson,
Volume 5, Issue 2 (9-2005)
Abstract
Background: Vascular factors in conjunction with metabolic issues are involved in both etiopathogenesis of diabetic neuropathy (DNU), and more remarkably in "repair" phase, when the net balance between neuroregenerative/degenerative reactions is dictated to some extent by these factors. The ischemic nature of DNU indicates the importance of re-establishment of blood vessels. VEGF, a growth factor which, in addition to its hemodynamic effects, possesses an "angiogenic" capacity has been the subject of extensive investigations in DNU, especially, interventional therapies. The impacts of racial and inherited backgrounds in the development of DNU suggest that the genetic issues partially govern the outcome of diabetic late complications, including DNU. By conducting a candidate gene case-control association study, present study explores the possibility if the inter-individual variations of VEGF gene structure by any means encode the genetic susceptibility/resistance in the course of DNU.
Methods: The distribution of VEGF gene polymorphisms frequencies were analyzed at positions –7*C/T, -1001*G/C, -1154*G/A and –2578*C/A and were evaluated by ARMS-PCR in 248 type 1 diabetic subjects (81 DNU+, 167 DNU−) and 113 healthy controls, all from "British-Caucasian" origin.
Results: When the frequency of the polymorphic alleles/genotypes between patients and controls, and also between two subgroups within patients' group with each other (DNU+ vs. DNU−) or with healthy controls were compared, only in one situation a significant difference was evident. The distribution of a VEGF gene polymorphism at promoter region (–7*C/T) at allelic (but not at genotypic) level was notably different between diabetics, with and without neuropathy, while the minor allele (T) conferred a protective effect (P=0.03 OR = 1.75).
Conclusion: The present study may imply a prognostic value for VEGF gene polymorphism at promoter region (–7*C/T) in DNU. However, it requires further studies to appreciate better the phenotypic impact of this polymorphism in this chronic complication of diabetes. A catalog of candidate genes polymorphisms that functionally reflect a protection/predisposition to DNU can provide the genotypic profile that can be useful to reasonably predict the overall behavior of diabetic subjects to the metabolic derangements relative to development of DNU, which in turn may require adoption of relevant preventive and therapeutic measures.
Javad Tavakkoly Bazzaz, Vera Pravica, Andrew Jm Boulton, Ian V Hutchinson,
Volume 5, Issue 3 (5-2006)
Abstract
Background: VEGF is newly discovered growth factor that has diverse biologic properties. The bottom-line of these activities is conduction and orchestration of a series of reactions that are taking place at microvasculature of different tissues/organs. Among the growth factors, cytokines and other mediators that reflect meaningful alteration in their local/systemic level, VEGF is the distinct player which can explain by its own all hemodynamic and architectural manifestations present in diabetic retinopathy (DR). The present study was conducted to pursue the role of a candidate gene structure variation, VEGF gene polymorphisms, in genetic susceptibility/resistance to development of DR through a cross sectional case-control study.
Methods: The frequency of four SNPs in VEGF gene at positions –7*C/T, –1001*G/C, –1154*G/A and –2578*C/A has been traced among 248 type 1 diabetic subjects (135 DR+, 113 DR−) along with 95 healthy controls. The populations had "British-Caucasian" background and ARMS-PCR technique was employed for DNA genotyping.
Results: Comparing the polymorphic variants' frequency at both allelic and genotypic levels among different groups/subgroups, significant difference was noticeable for –7*C/T polymorphism between diabetic patients with vs. without DR, while T allele conferred protective effect (p=0.002 OR=1.98).
Conclusion: Contemplating that up-regulation/over-expression of VEGF (local/systemic) as a common pre-requisite for DR development, our hypothesis was that whether the VEGF gene structural variations is correlated with the magnitude of VEGF expression in response to different stimuli present in diabetic context, namely hypoxia and hyperglycemia. Our data indicate that among the examined polymorphisms of VEGF gene, only SNP at position –7*C/T harbored significant difference between DR+ vs. DR− cases, proposing phenotypic impact for that SNP illustrating by evolvement/impediment of DR. However, reminding the insubstantial role of genetic issues in development of DR relative to DN or DNU, replicating current hypothesis by providing larger sample size and also employing more polymorphic markers could shed more light on the subject of present study.
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Volume 10, Issue 4 (10-2011)
Abstract
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Volume 11, Issue 5 (10-2012)
Abstract
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