Showing 16 results for Diabetic Rat
Ali Mohammad Sharifi, Seyed Hadi Mousavi, Bagher Larijani,
Volume 4, Issue 2 (8-2004)
Abstract
Background: Due to homeostatic and regulatory potentials of nitric oxide (NO) in vascular physiology, regulatory systems that determine NO bio-synthesis and bioavailability have been the subject of extensive research in molecular medicine. In the field of vascular system pathophysiology, endothelial nitric oxide synthase (eNOS) which is the major producer and regulator of NO in vascular tissues has received the most attention. Impairment of NO bioavailability (NO quenching) is a common feature in poorly controlled diabetics due to increased catabolism and decreased production of NO. Such impairment in severe forms could end to vasodilation breakdown in peripheral tissues (mainly in skeletal muscles) and defective regional blood flow, that in turn disturb insulin-dependent glucose uptake ensuing insulin resistance state.
Methods: The phenotypic impact of an eNOS gene polymorphism at position 786*C/T (that its functionality has been revealed already) on genetic propensity to diabetic retinopathy is evaluated in a British-Caucasian population with type 1 diabetes (T1DM).
Results: In contrast to genotypes, there was a significant difference in distribution of allele frequencies between T1DM patients (n= 249) and healthy controls (n= 104) (p= 0/036), that may imply eNOS and/or NO involvement in development of T1DM. Most notably a significant difference also was evident in allele frequency between retinopaths (n= 134) and healthy controls (p= 0/02). No significant difference was detected when the genotype/allele frequencies were compared between retinopaths (n= 134) and non-retinopaths diabetics (n= 115) (p=NS).
Conclusion: Our data is compatible with previous studies which demonstrated that allele C of eNOS 786*C/T polymorphism is associated with increased HbA1c levels. By emphasizing the phenotypic and prognostic value of the abovementioned polymorphism, our data calls for further investigations to find out whether this polymorphism can be employed as a genetic marker in clinical medicine to recognize high-risk diabetics at the time of diabetes onset/diagnosis.
Mahmood Khayatian, Bagher Larijani, Bijan Farzami, Shirin Pournourmohammadi, Hoda Boushehri,
Volume 6, Issue 1 (8-2006)
Abstract
Background: Sulfonylurea agents such as Glibenclamide (Glyburide) have been widely prescribe in treatment of type 2 diabetic patients for decades, but controversy remains about their precise mechanism of action. On the other hand, glucokinase serves as a glucose sensor in pancreatic β-cells and plays a key role in the regulation of insulin secretion and glucose homeostasis. The aim of the present study was to evaluate the effect of Glibenclamide on insulin secretion and glucokinase activity in the rat isolated pancreatic islets of Langerhans.
Methods: The islets from normal and type 2 diabetic rats were isolated by collagenase digestion method. Glucokinase activity was measured via determination the rate of glucose-6-phosphate formation in the fluorometric assay. Insulin secretion from hand-picked islets was evaluated by static incubation technique. Insulin concentration was measured by rat insulin ELISA kit.
Results: Our findings obtained from incubation of Glybenclamide with pancreatic islets revealed that this agent increases basal insulin secretion (at 2.8 mM glucose) in both normal and diabetic rats as compared it with control islet (without drug). However, the increase of insulin secretion in response to 16.7 mM glucose was not significant. On the other hand, Glybenclamide had no activating and/or inhibiting effect on pancreatic glucokinase activity in both diabetic and normal Rats. But reduced activity of this enzyme in diabetic rats was significant in comparison with normal.
Conclusion: These data show that increasing effect of Glybenclamide on insulin secretion is through a mechanism other than affecting Glucose Mediated Insulin Release. Moreover, the regulation of pancreatic glucokinase does not depend on glybenclamid.
Behzad Foruotan, Sahar Molzemi, Hoosin Harati Por, Shahram Molzemi, Nahide Bolbol Haghighi, Fatemeh Sadat Alam Al-Hoda, Amir Hossein Ashenaii, Seyed Reza Mousavi, Mohammad Reza Jafari, Mohsen Choopani Moghaddam,
Volume 13, Issue 5 (7-2014)
Abstract
Background: Finding effective drugs is considered for healing diabetic wounds by researchers. The purpose of this study is to evaluate the wound healing effects of palmatine in the healing of skin wounds in normal and diabetic rats.
Methods: In this study 16 male Wistar rats were dهvided into four groups (Negative control, Positive control, The first experimental,The second experimental) . A 4 cm2 wound was created in the left side of the spinal cord in each of these groups and the process of wound healing was macroscopically evaluated.
Results: The wounds of the diabetic groups (the diabetes was coused by streptozocin) showed longer healing process in comparison with the normal groups and the wound healing in the experimental groups which were treated by palmitine was faster in comparison with the control group.
Conclusions: the results showed that palmatine speeds the wound healing up in normal and diabetic samples.
Mehrnoosh Mahrou, Abbas Ali Gaeini, Sirous Chobbineh, Mohsen Javidi,
Volume 14, Issue 1 (1-2015)
Abstract
Background: Exercise is very important factor in control of diseases. It also has been suggested that angiogenesis inhibitor factor is controlled and angiogenesis simulative factor is intensified on those who are suffering diabetic and doing physical activity. This study is aimed to evaluate the effect of Eight-week Resistance Training on unbalanced Angiogenesis in Diabetic male rats.
Methods: Twenty-four diabetic male Wistar rats were divided into two groups of control and training. Resistance training protocol includes one set of 10 times per day climbing the ladder suffering a weight connected to each rat tail [with respect to the maximum volume carrying each rat] for 3days a week and for 8 weeks. After 48 hour of the last training session, blood samples were taken from rat's hearts and VEGF, NO, glucose and insulin were determined regarding to serum sample taken. Analytical statistics examined with the use of SPSS16 software and considering α< 0.05.
Results: this study of Eight-week Resistance Training resulted no significant increase on VEGF [P=0.776] and NO [p=0.946] in diabetic rats serum but there was a significant decrease in blood glucose [p=0.001] however, no significant difference was observed in insulin level between the groups [p=0.93].
Conclusion: Despite resistance training appears to improve glucose levels in diabetic rats it has no positive effect on the stimulation factors of angiogenesis.
Ghasem Sazegar, Vahid Ebrahimi, Mohammad Javad Saeedi Boroujeni, Shabnam Mohammadi, Ramin Salimnezhad,
Volume 14, Issue 1 (1-2015)
Abstract
Background: Given diabetes mellitus has an adverse effect on spermatogenesis and male fertility, the aim of the present study was to investigate the effect of L-carnitine administration on spermatogenesis and testicular structure in diabetic rats induced with Stereptozotocin.
Methods: In this experimental study, fifteen rats were randomly divided into 3 groups: control group was received citrate buffer. Case group was diabetic rats that received 40 mg/kg carnitine for 16 days. Third group was diabetic rats that did not receive carnitine. After sixteen days, morphometric study was performed. Besides, index of testicular weight was obtained. Then, data were analyzed using SPSS software and ANOVA.
Results: In testicular sections of diabetic rats were observed a disruption and deformity in first layer of somniferous tubules. Statistical analysis showed a significant difference in carnitine group when compared to the control group (P<0.05). There was a significant difference between weight of diabetic rat compared to the control group (P<0.001). Besides, there was the most ratio of testis index in control group.
Conclusion: Our finding showed that short-term L-carnitine administration was affective on the diameter and thickness of seminiferous tubules. Hence, L-carnitine treatment may cause an improvement in spermatogenesis of diabetic men.
Zahra Abbasizadeh, Mahnaz Kesmati, Hamid Galehdari, Anahita Rezaie, Seyed Mansor Seyednezhad,
Volume 14, Issue 6 (9-2015)
Abstract
Background: The process of wound healing is impaired in diabetes. Many efforts have been made to accelerate the wound healing process. Long time healing effect of a herbal complex containing Aloe vera, Myrrh, dragon’s blood and henna has been observed in wound healing, But sufficient scientific evidence of how the mechanism of action of this compound is absent in diabetic wounds Whereas the effect of each of them separately in several studies on ulcers observed. The purpose of this study was to evaluate the effect of topical herbal (briefly called Herbalin) on wound healing in diabetic rats.
Methods: The diabetic rats were divided into two groups: control (treated with Vaseline as a vehicle) and experimental (treatment with herbalin) were included. In each class, all wound round with a diameter of2cmwas made on the dorsal surface of diabetic rats. Wound measurement and histopathological parameters such as the formation of re-epithelization, granulation tissue formation and the average thickness of the epithelium at intervals of7, 14and21dayswere evaluated. Strain epithelium on day 14andthe wound length atday21was evaluated in the terminal phase.
Results: In macroscopic study, the Herbalin treated wounds were found to healing much faster and the day 14 has considerable change compared with control group (P<0.05). In microscopic study, in all cases of the Herbalin treatment groups showed a significantly increased as compared with controls (P<0.05).
Conclusion: According to the results, the herbal complex, possibly by accelerating the formation of granulation tissue and epithelium and thickening of the epithelium has an important role in wound healing in diabetic and reduces the time required for healing.
Seyed Mohammad Mazloomi, Mohsen Mohammadi Sartang, Nader Tanideh, Abbas Rezainzadeh,
Volume 14, Issue 6 (9-2015)
Abstract
Background: Type 2 diabetes is a major metabolic disorder. This study was aimed to evaluate the effect of probiotic soymilk fermented with Bifidobacterium lactis and containing omega-3 on hematologic, oxidative stress, anti-oxidant and inflammatory parameters in type 2 diabetic rats.
Methods: In this experimental manipulation study 65 rats were divided into five groups. Type 2 diabetes was induced by a single injection of streptozotocin and nicotinamide. Normal control (NC) and diabetic control (DC) rats received 1 mL/day of distilled water, and three groups of diabetic rats were administered 1 mL/day of soymilk (SM), fermented soymilk (FSM) and fermented soymilk fortified with omega-3 (FSM+omega-3) products by oral gavage for 28 days. Hematologic, oxidative stress, anti-oxidant and inflammatory parameters were measured at the end of experiment.
Results: At the end of experiment, in all treated diabetic rats compared with the DC group, red blood cell (RBC), hemoglobin (Hb), hematocrit and superoxide dismutase (SOD) concentrations increased significantly (p<0.05), and malondialdehyde (MDA) concentrations reduced significantly (p<0.05). The reduction of MDA and increasing in Hb, RBC, hematocrit and SOD concentrations in the FSM+omega-3 group was more than two other groups. In the FSM+omega-3 group, high sensitivity C-reactive protein (hs-CRP) concentrations compared with the DC and FSM groups were significantly lower (p<0.05).
Conclusion: The experiments suggest that combining soymilk, probiotics and omega-3 may be beneficial for the reducing oxidative-stress in type 2 diabetes.
Maryam Ahmadi, Gholam Hosin Vaezi,
Volume 14, Issue 6 (9-2015)
Abstract
Background: Diabetes accompanies with hormonal, biochemical changes, and metabolic damages are of body. So in this research, therapeutic effect of the blue mesocarp extract on streptozotocin diabetes has been discussed.
Methods: 30 adult Westar rats were divided into five groups six below. Witness group: that at the same time by injecting (STZ) to diabetic rats citrate buffer has been injected into them as the attribute. First experimental group: became diabetes by injecting 55mg/kg (STZ). Second experimental group and Third experimental group and Fourth experimental group: including six diabetic rats that were treated by injecting daily 100, 150& 200mg blue mesocarp extract of walnut end of week 13direct bloodletting from the heart has been achieved due to study of One way anova and a further test of TUKEY were consumed then samples were delivered to the lab for insulin hormone and blood sugar measuring
Results: In the treated group there was a meaningful decrease in blood sugar in all doses of blue mesocarp extract of walnut than diabetic group and also a meaningful increase in serum's ansoline level and body weight in treated group than diabetic group. This study for the first time showed that using blue mesocarp extract of walnut in long period, meaningfully decreases blood sugar in diabetic rats and it causes the meaningful increase of serum's ansoline in treated group.
Conclusion: diabetes redounds to decrease of ansoline hormone and increase of blood sugar by making free radicals and oxidative stresses. Blue mesocarp extract with ability of prevent and treating of oxidative stresses has an important role in improving the consequences of diabetes and also the increase of ansoline hormone.
Farideh Alah Gahi, Abdolhosin Shiravi, Vida Hojati,
Volume 15, Issue 3 (2-2016)
Abstract
Background: Finding effective drugs have been considered for healing diabetic wounds by researchers. The purpose of this study was to evaluate the effects on wound healing ointment Alcoholic extract of grape seed skin is normal and diabetic rats.
Methods: In this study 48 male Wistar rats into four groups (negative control, positive control, the first experimental, the second experimental) were divided. Groups tested in the injured area of 3 cm2 were created on the left spine. The wound healing was evaluated macroscopically. The results are reported as Mean ± SD. Statistical inference border (P ≥0.05) and (P ≥0.01) and (P ≥0.001).
Results: Compared with normal wound healing in diabetic groups were later and Wound healing in experimental groups treated with Vitis Vinifera more quickly than the control group, respectively.
Conclusions: In this study the skin ointment Vitis Vinifera accelerates the wound healing is normal and diabetic samples.
Mousa Khalafi, Ali Asghar Ravasi, Fatemeh Shabkhiz, Mohammad Moradi, Yashar Zarei,
Volume 15, Issue 4 (5-2016)
Abstract
Background: Irisin was identified as a myokine secreted by exercise which plays an important role in energy metabolism and regulation of metabolic diseases such as diabetes. The aim of the present study is to investigate the effects of high intensity interval exercise (HIIE) and moderate intensity continuous exercise (MICE) on serum irisin and Subcutaneous UCP-1 in diabetic male rats.
Methods: In this study, 29 diabetic Wistar rats (12 week- age, 220-240 gr- weight) were assigned to 5 groups: immediately and 2 hours after high intensity interval exercise (HIIE0, HIIE2), immediately and 2 hours after moderate intensity continuous exercise (MICE0, MICE2) and control (C). Both MICE groups performed on the treadmill with intensity 60-65% vo2max for 40 minutes and both HIIE groups with intensity 90-95% vo2max in the 12 interval-one minute period and 1 minute rest intervals. ELISA was used to measure serum irisin and subcutaneous fat UCP-1. One-way ANOVA and Tukey post hoc test has used to data analysis, the level of significance has been considered at P≤0/05.
Results: Data analysis showed serum irisin levels in the HIIE0 group and subcutaneous fat UCP-1 in the HIIE2 significantly increased compared to the control group (p˂0/05). However, there was no significant difference between other research groups (P>0/05).
Conclusion: It seems high intensity of exercise had important roles in significant changes of serum irisin levels and subcutaneous fat UCP-1in diabetic rats.
Elahe Dianati, Sahar Molzemi, Mohsen Aminian,
Volume 15, Issue 5 (7-2016)
Abstract
Background: In the event of damage to the skin, the epidermis of which goes to the disintegration of the body, is a wound that is common in diabetics. The purpose of this study was to evaluate microscopic effects of Aqueous extract of curcumin on wound healing in diabetic male rats.
Methods: In this study 48 male Wistar rats were divided into 4 groups (control, sham, experimental, empirical two) were tested. The extra pawn injured area of 3 cm to the left of the spine created. Wound healing was examined microscopic.
Results: The streptozocin diabetic wound healing compared to the control group later showed and wound healing in experimental groups treated with Aqueous extract of curcumin was faster than the control group.
Conclusion: The results showed that the Aqueous extract of curcumin skin accelerates wound healing in normal and diabetic samples.
Mahbobeh Hadizadeh, Sahar Molzemi, Mitra Bagheri, Mohsen Aminiyan,
Volume 16, Issue 2 (1-2017)
Abstract
Background: In the event of damage to the skin, the epidermis of which goes to the disintegration of the body, is a wound that is common in diabetics. The purpose of this study was to evaluate Effect of alcohol extract of Nigella Sativa on wound healing in diabetic male rats
Methods: In this study 48 male Wistar rats were divided into 4 groups (control, sham, experimental, empirical two) were tested. The extra pawn injured area of 3 cm to the left of the spine created. Wound healing was examined macroscopically.
Results: The streptozocin diabetic wound healing compared to the control group later showed and wound healing in experimental groups treated with alcohol extract of Nigella Sativa was faster than the control group.
Conclusion: The results showed that the alcohol extract of Nigella Sativa skin accelerate wound healing in normal and diabetic samples.
Masoumeh Hosseini, Maryam Hosseini,
Volume 19, Issue 3 (2-2020)
Abstract
Background: The proinflammatory cytokines of tumor necrosis factor alpha TNF-α and anti-inflammatory interleukin-10 have important roles in inducing and preventing systemic inflammation, respectively. The purpose of this study was to the synergistic effect of High-Intensity Interval training and resveratrol consumption on IL-10 and TNF- α in diabetic rats.
Methods: In this experimental study, 32 diabetic rats 225± 10 g based on Cohen's sample size estimation table diabetic with combination of high-fat diet and streptozotocin (STZ) injection, were randomly divided into four groups: control(C), supplement(M), training (T)and training + supplement(TM). The training groups were exposed to high-intensity interval training with an intensity of 90 to 100% VO2max for 8 weeks, 5 sessions per week, each session for 18 minutes. The supplement groups received 10 mg / kg of resveratrol daily for eight weeks, three times a week by gavage. Blood samples were taken 48 hours after the last training session. Data were analyzed using one-way analysis of variance and SPSS software version 24 (ANOVA).
Results: Results showed that IL-10 increased in the experimental groups but was not significant (P=0.109). TNF-α levels decreased in the TM group, whereas it increased in the T and M groups, which was significant compared to the TM group (P=.000). Fasting glucose, insulin and insulin resistance index decreased significantly in experimental groups (P=.000).
Conclusion: Eight weeks of high-intensity interval training plus resveratrol can have a positive effect on inflammation and insulin resistance by increasing IL-10 and decreasing TNF-α.
Elaheh Heidarnia, Farzaneh Taghian, Khosro Jalali Dehkordi, Mehrzad Moghadasi,
Volume 21, Issue 5 (1-2022)
Abstract
Background: The apoptosis process as a common status in heart injuries could be imposed significantly by hyperglycemia chronically. Therefore, the aim of this study was to investigate the inhibitory effects of concurrent training and antioxidant supplementation individually and in combination on
biogenesis and function of mitochondrial in the heart tissue of
diabetic rats with STZ.
Methods: In this experimental study, fifty male Sprague Dawley rats were classified into five groups (n=10 each group): healthy rats as control, diabetic rats, diabetic combined resistance/endurance training, diabetic rats which consumed supplementation vitamin E and C, and the combined supplementation and training. Here, we calculated changes in genes expression based on artificial intelligence methods and evaluated genes expression in apoptotic influencing combined training and antioxidants vitamins consumption in heart injured models by streptozotocin via Real-Time PCR. Combination training including, respectively, resistance training, 5 days a week with increasing intensity of 5-45% of rat weight and endurance training on treadmill increasingly from 10-30 minutes and intensity of 40-75% of maximum speed during 8 weeks. Data were analyzed by two-way ANOVA (P<0.05).
Results: the relative expression of PGC1α and Tfam were significantly decreased between healthy control and diabetes controls group. The results indicated that significantly increased of PGC1α and Tfam in the training(P<0.05), supplement(P<0.05) and combination groups(p<0.05). In addition, in this study, it was observed that supplementation and exercise had an increasing effect on the expression of PGC1α and Tfam genes. On the other hand, glucose concentration and weight of rats treated with supplementation and exercise were significantly reduced compared to other groups (P<0.05).
Conclusion: Based on the results, interaction between antioxidant supplements and exercise reduced the glucose concentration and improved the mitochondrial biogenesis of heart tissue, while the combination of these two interventions compared to the effect of each alone, the effect has more.
Leila Rahmani, Mohammad Rahman Rahimi, Shamseddin Ahmadi , Hassan Faraji,
Volume 24, Issue 4 (10-2024)
Abstract
Background: Glucose transporter4 (GLUT4) is the main glucose transporter in skeletal muscle. Impaired GLUT4 expression plays a role in the disorders of glycemic homeostasis. The aim of the present study was to investigate the combined effects of aerobic training and vitamin D3 supplementation on Glut4 protein levels and insulin resistance in the soleus muscle of diabetic rats with STZ and high-fat diet.
Methods: In 40 male Wistar rats type 2 diabetes was induced by 6 weeks high-fat diet followed by streptozotocin injection. Then rats were randomly divided into five groups: Healthy control (HC), Diabetic control (DC). Diabetes+Aerobic training (DAT), Diabetes+Vitamin D3 (DVD) and Diabetes+Aerobic training+ Vitamin D3 (DVDAT). The rats underwent eight weeks of aerobic training and vitamin D3 supplementation. 24h after last session of training and, the rats were anesthetized and soleus muscle was isolated for measurement of Glut4 protein concentrations and serum levels of insulin, glucose, vitamin D3 index were measured.
Results: One-way ANOVA showed that GLUT4 protein levels in DC group was significantly lower than HC group (P<0.001), but in DVDAT group was significantly higher than DC group (P<0.04) and DVD group (P<0.005). Also in DAT group was significantly higher than DVD (P<0.018). The HOMA-IR index also in DVDAT, DAT and DVD groups was significantly lower than DC group (P<0.001).
Conclusion: It seems that Eight weeks of aerobic training with vitamin D3 supplementation improves glucose metabolism in diabetic rats via increasing Glut4 protein levels and improving insulin resistance index.
Mrs Fateme Saebi, Hossein Abednatanzi, Mohamad Ali Azarbayejani, Mrs Mandana Gholami,
Volume 24, Issue 6 (2-2024)
Abstract
The aim of the present study was to study the changes in GLUT4 gene expression in soleus muscle tissue and insulin resistance index after HIIT and royal jelly in type 2 diabetic obese rats. Methods: The statistical sample of the present study was 36 obese diabetic male rats. After 20 weeks of feeding with a high-fat diet, the rats became diabetic by intraperitoneal injection of 25 mg/kg of STZ. Diabetic rats were grouped into 4 groups: control, intermittent exercise, royal Jelly, HIIT-Jelly. Eight weeks HIIT, five sessions per week with 2-minute HIIT of 80 to 90% and one-minute rest interval were performed. Royal jelly was given by gavage with a dose of 100 mg/kg before training. Findings: Data analysis using one-way and two-way analysis of variance and post-hoc test showed that compared to the control group, HIIT led to a significant decrease in glucose and insulin resistance index HIIT and royal jelly led to a significant increase in GLUT4 gene expression in soleus muscle compared to the control group (P=0.001). Conclusion: HIIT and royal jelly were effective in reducing insulin resistance index and expression of genes effective in glucose consumption in soleus muscle. Also, HIIT and royal jelly led to an increase in GLUT4 gene expression in the soleus muscle compared to the control group, which is important in glucose consumption in diabetics.
