Iranian Journal of

Backgrounds and Objectives:Total and Fecal coliforms (TC and FC), heterotrophic plate count (HPC), were counted by microbiological method and E.coli O157:H7 were detected through immunological and Real time PCRmethods inwater intake and all of units of Isfahanwater treatment plant (IWTP).
Materials and Methods: The microbial profile including TC, FC, and HPC, were monitored and turbidity and total organic carbon were analyzed in 8 locations of water intake, and unit operation and processes of IWTP, including, inlet, sedimentation, ozonation, and filtration and finished water. Immunological method through anti-serum kits and molecular method of RT-PCR were used to detect E.coli O157:H7 in the 8 locations and also the sludge of the sedimentation basin and filters backwash water of IWTP.
Results: Survival of E.coli O157:H7 in sludge sample of sedimentation basin was indicated by formation of agglutination particles in immunological method and through indicator probes in the RT-PCR method. However, E.coli O157:H7 was not detected in water samples of other units of IWTP. The removal percent of TC, FC, and HPC were: 59.5, 49, and 54.8 % in sedimentation basin 66, 45.8, and 57 % in ozonation: 98.8, 98, and 78.8 in the filtration and 96, 100, 91% in disinfection, respectively.
Conclusion: This study approved the existence of the pathogenic coliform, E.coli O157:H7 in the
sludge of sedimentation basin. Absent of E.coli O157:H7 in the finished water indicates that the existing units of IWTP could eliminate these pathogenic bacteria, before reaching the final units of the plant, including the filters and disinfection.

The objective of this study was to collect all the conducted studies on nitrate concentration in water resources of Iran. To achieve this purpose, the published papers in ISC and ISI journals as well as conferences and seminars were evaluated. The results of this survey showed that 116 studies have been carried out in 26 provinces of Iran. But,there was no published paper in Ilam, Alborz, South Khorasan, Kohgiluyeh and Boyer-Ahmad, and Lorestan provinces. According to these studies, the largest number of studies was performed in Hamadan province (14 cases), Khuzestan, and Mazandaran provinces (10 cases) and the least number of studies was conducted in Ardabil, Bushehr, Qazvin, Qom, and Kermanshah provinces. In Hamadan province, more than 1435 water samples were collected from water resources of this province, which is representative of large number of studies in this region. Maximum nitrate concentration (318 mg/L) was reported in Isfahan province and then in Zahedan city (295 mg/L), Sistan and Baluchestan Province. Based on the reported results in these studies, the nitrate pollution in water resources of Iran is at medium level. In most of the studies, high nitrate concentrations are due to lack of sewage collection network, discharge of urban and industrial sewage to water resources, and agricultural activities, which use high amounts of manure and fertilizer.