Iranian Journal of

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Background and Objectives: Dust phenomenon has adverse effects on the health of the population.Due to the increasing concentration of Kermanshah dust phenomenon in recent years, The aim of this study was to measure the concentration of the sensitivity coefficients between dust phenomenon hospitalization and mortality rates for heart and respiratory.

Materials and Methods: This was a descriptive study in which we studied the sensitivity coefficient of hospitalization and mortality rates for heart disease and respiratory to changes of dust concentration.. The study duration was during March-September 2010 and 2011. We collected the dust phenomenon data from Kermanshah Province Environmental Protection Department. The admissions and mortality of the cardiovascular and respiratory sufferers was collected from the Imam Ali, Imam Reza, and Imam Khomeini governmental hosiptals in Kermanshah.

Results: 1% increase in air pollution caused by the dust phenomenon will result in increasing about 0.5 % of the respiratory patients, 1% cardiac patients, and about 0.3% of the heart disease mortality. The relationship between dust phenomenon and respiratory sufferers was statistically not significant.

Conclusions: We found that respiratory and cardiovascular hospital admission has a high sensitivity to dust phenomenon. This sensitivity has increased from 2010 to 2011. This sensitivity was greater for males than females. At least, during March-September 2010, for every 100% increase in the concentration of dust phenomenon, cardiovascular mortality increased by 29%.

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Background and Objectives: Weather pollution, caused by Ozone (O3) in metropolitans, is one of the major components of pollutants, which damage the environment and hurt all living organisms. Therefore, this study attempts to provide a model for the estimation of O3 concentration in Tabriz at two pollution monitoring stations: Abresan and Rastekuche.
Materials and Methods: In this research, Artificial neural networks (ANNs) were used to consider the impact of the meteorological and weather pollution parameters upon O3 concentration, and weight matrix of ANNs with Garson equation were used for sensitivity analysis of the input parameters to ANNs.
 Results: The results indicate that the O3 concentration is simultaneously affected by the meteorological and the weather pollution parameters. Among the meteorological parameters used by ANNs, maximum temperature and among the air pollution parameters, carbon monoxide had the maximum effect.
Conclusion: The results are representative of the acceptable performance of ANNs to predict O3 concentration. In addition, the parameters used in the modeling process could assess variations of the ozone concentration at the investigated stations.

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Background and objectives: In this research, an innovative drinking water quality index for assessing water resources as “modified drinking water quality index (MDWQI)” was developed and applied for evaluating all of the groundwater resources utilized for community water supply in urban areas of Iran during 2011. Materials and methods: Twenty-three water quality parameters and relevant Iranian standards for drinking water quality were selected as input parameters and benchmarks respectively. The MDWQI is calculated using three factors including the number of parameters that excurse benchmarks, the number of measurements in a dataset that excurse benchmarks and the magnitude of excursions. The MDWQI scores range from 0 to 100 and classify water quality in five categories as excellent (95-100), good (80-94), fair (65-79), marginal (45-64), and poor (0-44). Results: According to the MDWQI value, about 95% of the groundwater resources were in the good condition and the others were in the fair or marginal condition also the best and the worst water quality of water resources were observed in Ardebil Province and Qom Province respectively. The three parameters of fluoride, magnesium, and nitrate recorded the highest rates of violation to be 74, 32, and 13% respectively. Conclusion: The nationwide average score of the MDWQI was 85 (good description). This study indicated that the MDWQI and its sub-indices could describe the overall water quality of water bodies easily, reliably and correctly and have the potential suitability for extensive application all over the world.

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Background and Objectives: Kidney patients in each dialysis cycle are exposed to extremely large volume of water, which is in direct contact with the patient’s blood. Hence, the occurrence of any type of contamination in the water used can be very toxic to patients. Thus, quality of water plays an important role in patient well-being. The aim of our study was to investigate the quality of water used for hemodialysis in Kashan Akhavan hospital.
Materials and Methods: This cross-sectional descriptive study was conducted on water quality used for hemodialysis in Kashan Akhavan hospital during Oct.-Nov., 2011. During the study a total of 26 water samples were taken from the raw water and inlet of hemodialysis instrument. Collected water samples were analysed for Heterotrophic plate count, residual chlorine, pH , K⁺, SO₄^2- , Na⁺, F- ,Ca²⁺ , mg²⁺ ,No^3- , Hg⁺ , Cd²⁺, Cr⁶⁺, Zn²⁺, Cu²⁺, Se²⁺ ,Co²⁺,Ag ⁺and As²⁺ according to the standard methods for water and wastewater examination.
Results: Our results showed that the concentration of copper, zinc, cadmium, lead, chromium, and silver were 0.4, 0.6, 0.07, 0.05, 0.08, and 0.04 µgL^-1 respectively. In addition, concentration of the chemical elements and heavy metals did not exceed the standard level in any cases. Moreover, no microbial contamination was observed in the samples analyzed.
Conclusion: Based on the results obtained, all water quality parameters in hemodialysis ward of Kashan Akhavan Hospital were compatible with AAMI (Association for the Advancement of Medical Instrumentation) water quality Standards and no health risk threatens the kidney patients.

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Background and Objectives: Increasing waste tiers production has made the recycling of this solid waste a critical issue in the world. On the other hand, it seems contamination of groundwater to the petroleum pollutant like gasoline is a great threat to the health of societies in developing countries. The main objective of this study was gasoline removal from aquatic environment by waste tire derived activated carbon. Materials and Methods: In this study for preparation of activated carbon from waste tires, KOH was used for chemical activation process. We used argon gas to prevent precursor oxidation . We applied N2 gas and BET isotherm for characterization of the prepared activated carbon texture. Isothermal and kinetic models were used for defining gasoline adsorption characteristic to adsorbent, and thermodynamic studies were used to determine the effect of temperature. Results: Characterization results revealed that SBET and VTOTAL were 111.702m2/g and 0.124cc/g respectively. Langmuir and pseudo-second order models were the best isotherm and kinetic models for prediction of the adsorbent behaviors to adsorb gasoline. The Gibbs free energy changes were negative at all temperatures applied. Conclusion: Produced activated carbon has desired surface area and adsorptive capacity for gasoline adsorption in aquatic environments and it seems preparation activated carbon from waste tiers is cheap, effective and environment friendly.

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Background and Objectives: Modelling and specifying mathematical equations to predict and estimate a bioprocess is one of the important applications of bioengineering. Objective of this study was to identify proper and exact equations to describe various changes in biodegradation of heavy fuel oil through investigating kinetic models and third parametric equation of Ch. Materials and Methods: To submit an exact and perfect enough statement, two different experimental conditions were used in which isolated indigenous bacteria from environment were employed. Experiments were carried out during ten days and microbial growth, mazut concentration, pH, and electric potential curves were drawn. Fitting data onto kinetic models and Ch equation resulted accuracy and their constants. Results: We found that kinetic models were not capable to present an accurate and appropriate statement under different conditions. On the other hand, Ch equation by extending very accurate equations could satisfyingly illustrate mazut, pH, and electric potential changes based on time and microbial growth. Conclusion: Ch equation by reason of using two variables for computation of third variable and correctly selecting variables could describe various changes in mazut biodegradation under different conditions via mathematical statements. Moreover, it is possible that this equation can be used to study other various phenomena in future.

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Background & Objectives: The aim of the study was to design and implement integrated solid wastes management pattern in Shahroud industrial area, to evaluate the results, and to determine possible performance problems. Materials & Methods: This cross - sectional study was carried out for 4 years in Shahroud industrial area and the implementation process included: 1. qualitative and quantitative analysis of all solid wastes generated in the area, 2. determining the current state of solid waste management in the area and identifying programs conducted, 3. designing and implementation of integrated management pattern including design and implementation of training programs, laws, penalties and incentives, and illustrating and implementing programs for all factories, and 4. monitoring the implementation process and determining the results. Results: Annually, 1,728 tons of solid waste is generated in the area including 1603 tons of industrial waste and 125 tons of municipal wastes. By implementing this pattern, two separated systems of collection and recycling of domestic and industrial waste were launched in this area. Moreover, consistent with the goals, the amount of solid waste generated and disposed in 2011 was 51.5 and 28.6 kg per 100 million Rials production respectively. In addition, 42 and 40% of the industrial waste and paper were recycled. Conclusion: Results showed that implementation of this pattern, i.e. running source separation, training programs, capacity building, providing technical services, completing chain of industries and strengthening the cooperation between industrial estate management and industrial units could greatly reduce the waste management problems.

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Background and Objectives: Water complexes including Jacuzzi are among the most popular water recreation centers that noncompliance with health standards can lead to increased microbial growth in water and thus the risk of infection. This study aimed at comprehensive study of jacuzzis water in Yazd city and comparing the results with national and international standards. Materials and Methods: This was a descriptive - analytical study that statistical society included all Jacuzzis of Yazd city. Sampling of jacuzzis was performed every two weakes for 3 months (Summer 2011). We tested the water samples for parameters such as temperature, pH, amount of residual chlorine, turbidity, alkalinity, hardness, the population of heterotrophic bacteria, Staphylococcus aureus, Pseudomonas aeruginosa, fecal streptococci, and fecal coliforms accordance with standard methods. The SPSS software and ANOVA, T-test statistical tests, and/or Kruskal-Wallis, and Mann-Whitney were used for description of data and the obtained results were analyzed by comparing with standards. Results: we found that the mean of residual free and combined chlorine was equal to 0.628 and 0.337 mg/l respectively. The alkalinityand hardness were 141.928 and 275 mg/lCaCo₃ respectively. Mean of pH, turbidity and temperature were equal to 7.88, 0.457 NTU and 38.121˙C respectively.Moreover, the mean population of fecal streptococci bacteria, heterotrophic bacteria, Staphylococcus aureus, Pseudomonas aeruginosa, and fecal coliforms were 20465.7, 120.77, 209.91 and 7131.4 in 100 ml respectively. Statistical analysis of data showed that there is a direct relationship between water turbidity and pH (Pvalue=0.018) however, population of heterotraphic bacteria and residual free chlorine had indirectl relationship (Pvalue= 0.026). Conclusion: According to the results achieved, the parameters of heterotrophic bacteria population and alkalinity had the least compliance with the standard indicating the need for continuous monitoring of physical, chemical, and microbial parameters of Jacuzzi water and control of the number and density of people using Jacuzzi and continuous monitoring of Jacuzzi water filtration and chlorination process performance.

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Background and Objective: Milk and milk products are very suitable medium for growing microorganisms (e.g. Bacillus cereus). B. cereus is spore former bacilli, which easily survives during pasteurization and makes several problems in dairy industries. The aim of this study was to investigate aerobic spore and B. cereus of receiving raw milk from three UF plants. Materials and Methods: Samples were gathered from raw milk transport tankers arrived to plants during 30 days in winter. Also, the swab test was used for detection of B.cereus residual on milk contact surfaces. Results: High contamination level of aerobic spores (AeSC) and especially B.cereus were found in most samples compared with the criteria established by national and international standards. Although total viable count (TVC) in samples from industrial farms (IF) was lower than those from traditional farms (TFs) and milk collection centers (MCCs), considerable AeSC and B.cereus were transmitted to the UF plants from IFs. The highest and lowest TVC and B.cereus were found in samples from IFs and MCCs, respectively. In addition, our investigation in IFs revealed that teats contamination to soil and feces, as well as contaminated bedding might were the most important sources of B. cereus and AeSC of raw milk. Moreover, the results of swab tests confirmed that the “cleaning in place” system may not remove B.cereus effectively. Conclusion: It seems that for classifying raw milk quality, AeSC might be used as a more effective quality factor than TVC. Management commitment is effective to improve quality by balance between the amount and quality of receiving raw milk. This leads to the lower contamination in dairy plants and final products.

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Background and Objectives: Optimization of mazut biocracking with different variables is one of the bioengineering applications in petroleum industry. The purpose of this study was to optimize biocracking of mazut by native microorganisms. Materials and Methods: To optimize mazut cracking, using Taguchi method we run 32 experiments using seven factors including amount of microbial inoculation, initial pH, surfactant, glucose, phosphor source, nitrogen source and sea salt each of them with four levels and factor of microorganism type with two levels for design of experiment using that 32 experiments were designed by them. Results: Results showed that microbial mixture, 0.016 OD600 microbial inoculations, pH 8.3, Tween80 concentration of 2 g/L, glucose concentration of 4 g/L, phosphate concentration of 5 g/L, ammonium concentration of 9 g/L and sea salt concentration of 0.5 g/L were optimized conditions for biocracking of mazut process. Conclusion: Optimized level for each factor was not essentially inevitably the highest or the lowest level. Based on the analysis of variance, phosphor source with 15.8% and pH with 14.8% had the highest effect among other factors however overally, error factor with 31.6% had the highest influence. Amount of microbial inoculation with 0.63% had the lowest effect on optimizing biocracking of mazut.

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Background and aim: Traditional cheese has a special place in the diets of our community because of cultural, favorite taste, odor and its nutritional values as an important protein source. However, its pathogenic infection can endanger the human being health and cause serious economic losses. The aim of this study was measurement and determination of microbial infection in traditional cheese distributed in Markazi province in 2010. Material and methods: Markazi Province was divided into 10 districts eight sample were chosen from each district at summer, 2011. Samples were transferred to the laboratory under sterile conditions and were analyzed by microbiological tests. The data were analyzed statistically by T-test using SAS software. Results: The results indicated that all of samples had coliform, staphylococcus aurous, mold, and yeast contamination greater than Iranian standards (P<0.001). It was found that 34 percent of the samples had E.coli contamination moreover, 24 and 8 percent of samples had Coagulase-positive staphylococcal and salmonella contamination respectively. Conclusion: These results indicate a notable contamination of traditional cheese with microbial infection in Markazi province.

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Background: FMEA is one of the best known system safety techniques widely used as an identification tool. The purpose of this study was to apply FMEA method to identify and assess the risks posed by power lines in a residential area is Hasyrabad, Ahwaz. Materials and Methods: First, we identified the power line process and the equipment involved. Later, we determined the failure modes of equipment, which leads to the risk in the area studied. In order to measure the magnetic field lines, we used 3D EMF TESTER. Risks identified were scored based on the FMEA and for each case we calculated RPN. Then, with the help of SPSS software, we calculated level of risk and the cases identified were prioritized and analyzed based on the risk level. Results: The highest RPN values were associated with health risks and all risks involved in this group are at emergency conditions. It was revealed that wires (loss of privacy) and power transmission line (non-compliance with privacy standards) had highest value (RPN 360) and disconnect switch had lowest value (RPN 60). Conclusion: Due to the high risk in most cases, control measures were proposed based on the expertise in the safety-related equipment, training operators, and regulatory measures in order to reduce the level of risk.

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Background and Objectives: The present study was designed to determine total mercury concentrations in sediment and the soft tissues of two bivalve species, Saccostrea cucullata and Callista umbonella, in coastline of Bandar Abbas in 2011. Materials and Methods: Generally, 67 bivalves and 10 sediment samples were collected from two stations (terminal of Bandar Abbas and tourism park of Soro). We measured total mercury concentrations in each sample using Mercury Analyzer (Leco AMA 254). Results: Means of mercury concentration were 145.58 and 151.13 (ng/g dw) in soft tissues of oyster S. cucullata and 34.5 and 63.95 in the soft tissues of Clam C. umbonella in two stations, terminal of Bandar Abbas and tourism park of Soro respectively. Total mercury concentrations were 30.27 and 20.55 (ng /g dw) in the sediment samples in two stations, terminal of Bandar Abbas and tourism park of Soro respectively. A significant difference was found in mercury concentrations between soft tissue of the two species (p <0.01). Conclusion: Considering higher concentration of mercury in the soft tissues of oyster S. cucullata than clam C. umbonella, lower coefficient of variation in soft tissue of oyster relative to clam C. umbonella, and also significant correlations between mercury levels in the soft tissue of oyster and sediments, it can be concluded that the soft tissues of oyster S. cucullata can be used as biomonitoring agent for mercury in intertidal zone of Bandar Abbas. In comparison with food safety standards (FDA, EU Commission and NHMRC), mercury concentrations in oysters were well within the permissible limits for human consumption.

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Background and Objectives: Dez dam located on Karun River is one of the largest Iranian dams. In a field study, EC, NO₃, NH₄, PO₄, turbidity, TS, alkalinity, coli, temperature, chlorophyll a, DO, BOD₅, and Secchi disk (at depths of 0, 2.5, 5, 10, 25, and 50 m)were measured at five sampling points during 2011-2012 water year. Materials and Methods: Water Quality Index and TSI were calculated for all sampling points and quality zonation was conducted in GIS. Results: It was found that the best water quality index for reservoir water is at second sampling point with amount of 61 situated at the center of reservoir, and the worst index is at entering point to the reservoir with amount of 46 in April. The main reason of changes was turnover of thermal stratification. Conclusion: The laboratory analysis and assessing the quality indices revealed that the Dez reservoir water is suitable for variety of public uses, however, it needs advanced treatment for drinking purpose. Moreover, in the case of continuity of pollutant entrance into the reservoir, the eutrophication risk would threaten the reservoir water quality. Eutrophication occurrence will cause serious limitations in water use applicability, increase turbidity, produce toxic materials, and increase sedimentation regime.

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Background and objectives: Because of problems dealing with bioremediation including being time consuming, low efficiency and toxicity to biota, application of advanced oxidation processes with higher efficiency and shorter remediation time have been considered for removal of hydrophobic hydrocarbons from contaminated soils. A great interest has been directed to Fenton oxidation because of its simplicity and high oxidation potential. The objective of this study was to determine the Fenton-like oxidation efficiency for pyrene removal from soil using iron nano oxides and Fe²⁺. Material and Methods: The H₂O₂/Fe molar ratios of unadjusted with native Fe content of soil, 10, and 20 H2O2 concentrations of 0 – 500 mM pH 3, 5, and 7 and soil samples containing Fe²⁺, native iron and iron nano oxides were investigated for removal of 100 mg/kg pyrene according to Taguchi experimental design. Results: Fe²⁺, H₂O₂/Fe molar ratio of 20, pH 3 and H₂O₂ concentration of 500 mM were determined as optimum conditions. Under optimum conditions, S/N ratio increased to 39.322 and the pyrne removal reached to 86 % for Fe²⁺ and 83 % for Fe³⁺ respectively, after 2 hours of reaction time and pH 3. Conclusion: Fenton oxidation using iron nano oxides under defined optimum conditions and neutral pH, can be a suitable alternative to conventional Fenton for remediation of soils contaminated with pyrene.

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Background and objectives: Dichloromethane (DCM) is one of the hazardous contaminants of the environment, especially ambient air that threatens human health at both acute and chronic exposures. In this study, the performance of a pilot-scale hybrid bubble column/biofilter (HBCB) bioreactor was studied for the removal of DCM from waste gas streams at steady state. Materials and methods: The experiments were conducted in four stages with relatively constant concentrations of DCM (approximately 240 ppm) and variable empty bed residence time (EBRT) of 50, 100, 150 and 200 s. In addition to determining DCM removal rate and efficiency, quality parameters of mixed liquor of the bubble column bioreactor were studied and kinetic of biofiltration was analyzed. Results: The average DCM removal efficiency of the HBCB bioreactor at EBRT of 200 and 150 s were 79 and 71% respectively. However, further reduction of EBRT resulted in significantly decreased DCM removal efficiency, so that at EBRT of 50 s, the DCM removal efficiency decreased to 32%. In addition, the EBRT reduction from 200 s to 50 s through increasing DCM loading rate resulted in increasing DCM removal rate from 12.1 to 19.6 g/m³.h. The results of kinetic analysis showed that the kinetic data of biofiltration were in the best fitness with the first order rate equation (R²>0.99 and &epsilon%<2.2) and the DCM removal rate constant was determined 0.0114 s^-1. The mixed liquor characterization indicated that the daily adjustment of pH and EC was sufficient to prevent any limitation in the performance of the HBCB bioreactor. Conclusion: This study indicated that the DCM removal rate and efficiency of the HBCB bioreactor were relatively high and the HBCB bioreactor had reliable performance during the variable operational conditions.

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Background & Objectives: Determining the impacts of air pollution in cities is facing two major challenges first, the limited data on the health effects of pollutants, and secondly, the lack of information on exposure to air pollutants and their concentration. This is an applied-observational carried out to determine the concentration, seasonal changes, and to estimate the health impacts attributed to the particulate matters in Tabriz during September 2012 to July 2013. Materials and Methods: The particulate matters were sampled using high volume sampler and were analyzed using portable HAZ-DUST EPAM-5000. The health impacts of particulate matterswere estimated using AirQ software developed by W.H.O. Results: The annual mean concentration of TSP, PM₁₀, PM_2.5, and PM₁ in the urban sampling site were 139, 83, 38, and 27 µg/m³ respectively. In addition, these concentrations were 178, 110, 40, and 27µg/m³ in industrial area. The average of PM₁₀/TSP, PM_2.5/PM₁₀, PM₁/PM₁₀, and PM₁/PM_2.5 ratio level was 0.6, 0.48, 0.33, and 0.7 in the urban areas and 0.61, 0.37, 0.28, and 0.77 in the industrial areas respectively. The total mortalities associated with the TSP, PM₁₀, and PM_2.5 concentrations were estimated as 327, 363, and 360 respectively. Nevertheless, the calculated cardiovascular mortality for TSP and PM₁₀ were estimated to be 202 and 227 respectively. Conclusion: This research found that the concentrations of PM₁₀ and PM_2.5 were 73 and 69% more than National Standard and 8 and 5% more than USEPA Standards respectively. In Tabriz, especially industrial area, the soils of surrounding ground and re-suspension of particles fromcontaminated soils have a significant contribution to particulate emissions.

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Background & Objectives: Entering LAS through the wastewater plant’s effluent to water resources causes taste and odor changes, aquatics death, oxygen transfer limitation, and disorders in water treatment processes. Therefore, the study objective was to determine optimum conditions for fenton oxidation process to remove linear alkyl benzene sulfonate from aqueous environments using taguchi method and its requirement evaluation for additional treatment of Biolac effluent. Materials &Methods: LAS removal using Fenton oxidation was evaluated in a 500 mL laboratory-scale batch reactor. In order to save the costs, to determine the optimum conditions of the Fenton oxidation, 25 runs were computed using Taghuchi method by Minitab 16 software. Sampling and required tests were performed based on standard methods examination for water and wastewater. For evaluation of Biolac process sufficiency in LAS removal, biolac efficiency in Urmia Wastewater Treatment Plant was studied in 2012. Results: Based on the LAS removal and chemicals required, Minitab software (Ver. 16) recommended the optimum conditions of Fenton oxidation at 900 mg/L H2O2, 170 mg/L Ferrous ion, pH of 4, and reaction time of 20 minutes. It was found that the Fenton oxidation for LAS removal had 86.5% efficiency under optimum condithions and it was second order reaction with the rate coefficient of 0.0152 L/mg.min. Biolac process does not need any additional treatment due to meeting LAS standard in municipal wastewater treatment. Thus, this process decreased annually mean LAS from 5.28 mg/L to 0.734 mg/L in municipal wastewater. Conclusion: Although the Fenton oxidation appears as a chemical process with high efficiency in the removal of LAS, the low efficiency of COD corresponding to the LAS indicated partial decomposition of linear alkyl benzene sulfonate by Fenton oxidation.

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Background and Objectives: Although amount of hospital waste generation is less than urban waste generation, but because of health risks involved, these wastes need to be managed precisely and specifically. Therefore, the purpose of this study was to survey quality and quantity of waste generation in Emam Khomaini Hospital, Karaj, Iran and to estimate its generation process in future. Materials and Methods: the total amount of different waste percentage and its weight were examined and analyzed through monthly sampling from segregated waste (including general, infectious, pharmaceutical, and sharp wastes) in Emam Khomaini Hospital in Karaj during four years (2010-2013). Results: the hospital waste generation rate became double during four years of study. Among different types of waste, the highest monthly averages per bed for general and infectious waste was 48.3 and 46.5% respectively. In addition, the most and least amount of waste generation was observed in April and September respectively. Analysis of variance showed that the increasing trend of waste generation had a significant difference (P<0.001). Moreover, the estimate of waste generation process through ARIMA time series model represented continuation of increasing trend of waste generation up to 6.3 (kg bed^-1 day^-1) on December 2014. Conclusion: Waste generation in Emam Khomaini’s hospital follows an increase trend ( average generation from 2.98 to 4.44 kg bed^-1 day^-1 in 2010 to 2012 respectively, reflecting the increasing trend in the waste generation. ARIMA time series model shows that the waste generation rate would be 1.5 fold more in autumn 2014. Estimate of waste generation is necessary for planning and designing of waste management.

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Background & Objectives: Cryptosporidium parvum is considered as one of the pathogenic agents transmitted by water, high resistance to conventional disinfection methods, and potency of creating various problems in water resource. Because of various problems in Cryptosporidium parvum studies, Bacillus subtilis spore is recommended as a surrogate organism for studying protozoa inactivation and evaluation of water quality. On the other hand, electrochemical process is presented as an environmental friendly and high efficient method in disinfection in recent years. The aim of this study was to propose a method for promotion of the water quality. Materials & Methods: In this study, the electrochemical system used was consisted of steel electrodes (4×8 cm), 200 mL volume, and 1-4 mg/L sodium chloride. The bacterial suspensions of Bacillus subtilis (ATCC 6633) was prepared according to the McFarland method with 103 to 106 spores/mL concentration. The microbial agent removal was evaluated by sampling and transferring water to the tripticase soy agar medium every 15 min for 60 min. The number of bacteria spores, supporting electrolyte, induced current, and reaction time were evaluated. Results: The proposed electrolysis process could not eliminate Bacillus subtilis spores at 104 to 106 spores mL^-1 rate at lower than 100 mA current for 60 min. Adding sodium chloride supporting electrolyte up to 4 mg/L concentration completely eliminated Bacillus subtilis spores after 60 min. Conclusion: Adding sodium chloride as a supporting electrolyte can increase the spore removal because of increasing direct and indirect oxidation in electrolysis process. Improving water disinfection and spore removal after 60 min could be described by higher oxidant agents in anode electrode.