Iranian Journal of

Background and Objective: The bibliometric analysis is the most effective way to study the scientific output and trend of research. The purpose of this study is to analyze the scientific output of the Institute of Environmental Research (IER) at Tehran University of Medical Sciences using bibliometric technique.
Materials and Methods: All related IER articles that have been indexed in Scopus and WoK were retrieved from the beginning of 2009 till November 23, 2019. The bibliometric data were extracted and analyzed using R software.
Results: The document production follows an increasing exponential trend with a rate of 10% per year. 807 documents were retrieved with an average citations of 19.26 per each document. The documents per author and authors per document were obtained 0.196 and 5.1, respectively. The highest citations to documents was in 2017 (35.5 citations). The most frequent keywords were found “Adsorption”, “Drinking water”, “Air pollution”, “Particulate matter”, “Risk assessment” and “Response surface methodology”. Thus, engineering-based studies are more prominent in the published studies. Health-based studies, including risk assessment studies, accounted for the most area of research. The largest numbers of international research collaboration were recorded with the United States, Switzerland, Canada and India. While, in the national scale, Shahid Beheshti University of Medical Sciences’ collaboration was prominent.
Conclusion: It is still necessary to promote the level of the IER’s international collaborations to raise its regional status. In addition, more attention should be paid to health-based topics like risk assessment studies. These studies need to be given higher priority in the action plan of the IER’s centers and research groups.

Background and Objective: One of the most important factors affecting communities’ health is safe drinking water. The UN general assembly considers “ensure availability and sustainable water and sanitation for all “as one of the 17 goals of SDGs for the 2030 development agenda.  Target 6.1 of SDGs aims to provide universal and equitable access to affordable drinking water and to provide safe drinking water for all households. Target 6.2 calls for an end to defecation in an open environment to ensure that each person has access to the toilet and safe disposal system. In current study, 143 Savadkooh villages with defects in water supply systems and health services were selected to update the indicators and implement sustainable development goals.  
Materials and Methods: A checklist was designed to extract targets 6.1 and 6.2 based on the Joint Monitoring Program (JMP). Considering 7400 households, margin of error of 5%, confidence limit of 95%, and the most conservative mode for response distribution (50%), the sample size was determined (512 households). Samples were collected randomly from all villages and then analysis was performed by SPSS 19.
Results: In drinking water sector, 84% of people have access to safe services, while only 15% and 1% have access to basic services and unimproved services, respectively. In sanitation sector, 98.8% of people use safe services though only 0.2% utilize limited services. In hygiene sector, 98.8% of people are provided with safe services.
Conclusion: Lack of drinking water safety and failure to improve water resources’ quality are the main problems in the drinking water sector. Lack of access to sewage network, wastewater disposal in the land is the biggest health hazard in the region. This study can provide solutions to improve the health quality of affected community.

Background and Objective: Microbial fuel cell (MFC) is a new green technology that uses the catabolic ability of microorganisms to produce bioenergy while simultaneously removing organic matter and other wastewater contaminants. Electrode material is one of the factors affecting the performance of microbial fuel cells. The aim of this study was to investigate the performance of microbial fuel cells in COD removal and bioenergy production from synthetic and real beverage wastewater.
Materials and Methods: In this research, a two-chamber microbial fuel cell with Nafion membrane and aerated  cathode was set up using two electrodes made of carbon felt and flat graphite after being contacted by synthetic wastewater with a concentration of COD 5000  mg/L and real beverage wastewater. Organic matter removal efficiency and voltage, power density and maximum current were determine.
Results: Experimental results showed that maximum COD removal efficiency of 92 % was achieved in synthetic wastewater and with a carbon felts electrode. In this condition, maximum voltage, power density and output current density of 469 mV, 175.28 mW/m², and 855 mA/m², were obtained, respectively. However, by using real industrial wastewater (beverage), maximum removal efficiency of COD, voltage, power density and output current density, related to carbon felt electrodes ‎were obtaines as 84 %, ‎460 mV, 91/65 mW/m², and 635 mA/m², respectively.
Conclusion: The findings showed that synthetic wastewater outperforms microbial fuel cells in terms of bioelectric production and organic matter removal as compared to real wastewater (beverage). The reason for the decrease in the cell performance might be the presence of solids and other confounding pollutants in real wastewater.

Background and Objective: Researchers investigate and identify the gaps and frequency of studies related to specific knowledge by using scientometrics. The purpose of this study was to analyze the trend of scientific research in the field of climate change in Iran.
Materials and Methods: All articles affiliated with Iran in the field of climate change have been cited in Scopus databases from 1991 to December 9, 2019, and were retrieved by using keywords like "climate change", "global warming", "global change", "climate variability", "greenhouse gas". The data were analyzed using R software.
Results: The total number of documents was 2253. The trend of document production in the field of climate change in Iran follows an increasing exponential trend. Iran's collaboration with other countries in the field of climate change studies was significant so around 25% of scientific productions have been produced jointly with other countries. Keywords analysis showed a greater frequency for keywords including “downscaling”, “energy”, “global warming” and “uncertainty”. However, despite the importance of issues such as “adaptation” and “vulnerability” concerning climate change in Iran, the frequency of these keywords in climate change studies in Iran is low.
Conclusion: Based on the results of this study and considering the importance of climate change and its effects on the country, also the importance of greenhouse gases including CO_2, and the rank of Iran as the eighth country in the emission of these gases, it is recommended to pay more attention to issues such as “vulnerability”, “adaptability”, analysis of “greenhouse gases production”, “management of energy consumption” and the use of “renewable energies” in future studies.
 

Background and Objective: Personal hygiene and adherence with health protocols in food preparation and distribution centers by operators are essential for controlling the transmission of infectious diseases. This study aimed to investigate the microbial contamination and compliance with health protocols among bakery workers in Mahmoudabad, Mazandaran, in 2020.
Materials and Methods: In a cross-sectional descriptive study, 149 samples were collected from the hands of workers in bakeries in Mahmoudabad. The microbial load was determined using the System Sure Plus device. Additionally, standard checklists, which included questions related to compliance with health protocols in the workplace, were used.  The collected data was then analyzed using SPSS 26 software.
Results: Among the studied samples, 51.1%had a microbial load higher than 30 RLU (Relative Light Units), 28.86%  had a microbial load of 10-30 RLU and 20.13% had a microbial load less than 10 RLU. Based on the results of the checklists, the rates of adherence to hand disinfection protocol, observance of social distancing, use of liquid soap, and use of a mask were 43.6%, 61.7%, 59.7%, and 53.7%, respectively. Additionally, data analysis revealed a significant relationship between business gown usage and microbial contamination on personnel's hands (p = 0.031).
Conclusion: Based on the results, it was evident that the workers' hand hygienewas in a concerning condition, posing a potential threat to their overall well-being. Therefore, individual health education and food safety practices should be improved, and inspections by environmental health monitors should be considered to ensure stricter public health standards.
 

Background and Objective: There have been concerns about microbial contamination along the coastal cities in the north of the country, particularly in cities of Mazandaran province, which could pose health risks for swimmers and tourists. The present study aimed to quantitatively assess the risk of gastrointestinal diseases resulting from exposure to E. coli and enterococci bacteria during recreational activities on the beaches of Fereydunkanar.
Materials and Methods: In this cross-sectional descriptive study, 66 grab samples collected during the summer were analyzed to investigate microbial contamination in the recreational beach waters of Fereydunkanar.The Monte Carlo simulation method was used to calculate both daily and annual infection risks.
Results: The findings demonstrated that transmission of enterococci was more likely than that of E. coli in the swimming areas of these beaches. The average annual infection risk for E. coli was 0.41 for adults and 0.69 for children . Additionally, the annual risk of enterococci infection was 1 for adults and 0.99 children and, which exceeds the range advised by the WHO and EPA. These findings indicate that children have a higher infection risk compared to adults.
Conclusion: Unrestricted discharge of municipal and industrial wastewater effluents into sea and rivers can contribute to the presence of harmful microbes in beaches. By implementing effective environmental and recreational management strategies, it is possible to minimize the risk of public health hazards at recreational beaches while also preserving the natural beauty of these important public spaces.
 

Background and Objective: Littered waste is one of the most common forms of pollution in urban environments, primarily caused by citizens’ behavior in waste disposal. This study aimed to investigate the density and composition of littered waste in Behbahan, Iran, in 2023 and assess the pollution status using a specific environmental index.
Materials and Methods: This descriptive cross-sectional study evaluated the density and composition of littered waste in Behbahan's urban environment using visual surveys. The findings on the density and composition of abandoned waste were interpreted using the Clean Environment Index, an environmental ranking method that assesses the severity of litter pollution.
Results: The study revealed that the density of littered waste in the surveyed areas ranged from 0.011/m² to 0.954/m². The average density of littered waste in commercial, residential, and recreational land uses was 0.809/m², 0.196/m², and 0.011/m², respectively. Cigarette butts were the most common type of litter, constituting 61% of the total waste on average. The Clean Environment Index values for the surveyed locations ranged from 0.39 to 32.56, indicating that 63.6% of the sites were categorized as clean or moderately polluted, while 36.3% were classified as extremely polluted.
Conclusion: Given the significant proportion of cigarette butts in the composition of littered waste in Behbahan and their impact on the pollution index, efficient management strategies for this type of waste are crucial. The finding that over one-third of the surveyed locations fell into the "extremely dirty" category highlights the need to enhance the efficiency of the urban cleaning system.
 

Background and Objective: A large amount of waste is generated from shrimp farming, primarily consisting of shrimp heads and shells. These wastes are often discharged into aquatic environments, where they act as pollutants and threaten the survival of other aquatic organisms. Therefore, reusing shrimp waste can help reduce water pollution and mitigate its environmental impact.
Material and Methods: In this study, carotenoids from shrimp waste were extracted using three methods: acidic, enzymatic, and alkaline. The amount of carotenoids was quantified for each extraction method. Their antioxidant effects on crude sunflower oil, in the presence of copper sulfate, were then evaluated using the TBARS assay. In this method, malondialdehyde levels are measured as an indicator of lipid oxidation. For this purpose, equal amounts of the extracted carotenoids were added to crude sunflower oil along with copper sulfate and placed on a shaker for 24 hours. Subsequently, the malondialdehyde content was measured.
Results: The highest carotenoid yield was obtained using the alkaline extraction method. In the TBARS assay, the alkaline method also demonstrated the greatest inhibition of lipid peroxidation in the presence of copper sulfate.
Conclusion:  It can be concluded that the alkaline method is a simple and cost-effective approach for extracting carotenoids from shrimp waste. These carotenoids are bioactive and valuable compounds with potential applications in the pharmaceutical and food industries.
 

Background and Objective: Heavy metal contamination in vegetable oils is a major food safety concern due to its potential adverse effects on public health. This study aimed to measure the concentrations of heavy metals (As, Pb, Cu, and Fe) in various types of vegetable oils and to assess the associated health risks from human consumption.
Materials and Methods: In this study, 72 samples of vegetable oils—including sesame, sunflower, and rapeseed—were systematically collected from markets in Sabzevar. The oil samples were accurately weighed and digested using a mixture of nitric acid, sulfuric acid, and hydrogen peroxide. The resulting digested solutions were filtered and analyzed for Pb, As, Cu, and Fe concentrations using microwave plasma atomic emission spectrometry (MP-AES). All procedures were performed in triplicate, following national Iranian standards. To accurately evaluate health risks, the hazard quotient (HQ) for non-carcinogenic effects and the lifetime cancer risk (LTCR) were calculated using Monte Carlo simulation (MCS). A daily intake of 0.227 kg of vegetable oils was assumed, based on national dietary data, to estimate chronic exposure.
Results: The results showed that the highest levels of heavy metals in vegetable oils were as follows: Pb (0.058 mg/kg) in sesame oil, As (0.090 mg/kg) in sunflower oil, and Cu and Fe (0.143 mg/kg and 0.847 mg/kg, respectively) in rapeseed oil. The Target Hazard Quotient (THQ) values for Pb (THQ = 0.146), Cu (THQ = 0.022), and Fe (THQ = 0.01) were within the safe range. However, the THQ for As (THQ = 1.905) was found to be significantly elevated. The cancer risk assessment indicated that the consumption of these oils is generally within the acceptable risk range, but the risk associated with As was estimated to be approximately 100 times higher than that of Pb.
Conclusion: The results of this study suggest that, although the carcinogenic risk associated with vegetable oil consumption is low, continuous monitoring of these products—particularly for heavy metal contaminants—is essential to ensure consumer safety.
 

Background and Objective: Swimming pools are considered as a potential source of microbial and chemical contamination due to direct and continuous contact with different groups of people. The present study was conducted to investigate the water health indicators of swimming pools in Kashan and Aran-Bidgol and compare them with the existing standards in the country in the years 2017-2021.
Materials and Methods: In this descriptive-analytical study with a longitudinal design, 830 samples were taken from all indoor and active pools in 2017-2021. Physicochemical parameters, and microbial parameters were measured. Finally, the data were analyzed using SPSS software 16.
Results: The concentration of free residual chlorine, combined chlorine, pH, turbidity, and corrosivity were in the desired range in 53 percent, 48 percent, 67 percent, 81 percent, and 67 percent of the water samples, respectively. None of the samples were contaminated with Pseudomonas. A significant and inverse relationship was observed between the bacterial population and free residual chlorine, and a significant and direct relationship was observed between the bacterial population and turbidity (p<0.005).
Conclusion: Due to the low compliance of the water chlorine level with the standard and the observation of coliform bacteria, Staphylococcus aureus, and heterotrophs, it is necessary to continuously monitor the physicochemical and microbial parameters of the water, as well as to carry out proper health surveillance and disinfection.