M Ali, F Ghiasi, H Badakhshan,
Volume 7, Issue 1 (7-2014)
Abstract
Background and Objectives: In the recent years, we confront to harmful effects of toxins such as herbicides on aquatic species due to irregular consumption of these compounds in agricultural operations and drainage of them to water ecosystems. In the present study, the effect of 2,4-D + MCPA “the frequently used herbicide in Kurdistan province” was assessed on the hematological parameters and liver enzymes in rainbow trout as the main aquatic species farmed in this area. Materials and Methods: After determination of LC50 using Probit model, 60 healthy trout fish with an average weight of 97 g were divided into two groups. The first group was considered as control and in the second treatment group, 1 cc/L herbicide (equivalent to 360 mg/L 2,4-D + 315 mg/L MCPA) was used. After 72 hours, hematology parameters including total number of red and white blood cells, differential count of white blood cells, hematocrit, and serum levels of ALT and AST enzymes were measured. Results: The values of blood tests including leukocytes, monocytes, and eosinophils in the toxin group was significantly increased in comparison with control group, whereas, the values of lymphocytes, erythrocytes and hematocrit were significantly decreased in toxin group compared with the control (p<0.05). There was no difference between the level of neutrophils in the treatment and control groups. The levels of liver enzymes, ALT and AST, in the treatment group increased significantly compared with the control group (P<0.05). The mortality rate after of 72 hours was 25% in the group treated with the toxin. Conclusions: Erythrocytes and hematocrit amounts of blood in rainbow trout were decreased due to exposure to 2,4-D + MCPA herbicide that eventually leads to oxygen deficiency and inefficient blood supply. The contact of red blood cells and hematopoietic tissues to toxin and destruction of them are led to loss of the cells in the blood. On the other hand, liver, kidney and gills autopsy of the wasted fish and the increasing of liver enzymes in the blood and tissues showed that exposure to the toxin lead to damages in fish blood cells and tissues.
N Naqshband, M Askari Hesni,
Volume 10, Issue 2 (9-2017)
Abstract
Background and Objective: Glyphosate is a non-selective, systemic herbicide that can control most plants, but it has harmful effects on organisms, especially aquatic animals. Static bioassays were made to determine acute toxicity of glyphosate and its effects on haematological parameters and behavior changes of common carp (Cyprinus carpio).
Materials and Methods: Common carp were exposed to different concentrations of glyphosate (5, 10, 20, 30 and 40 mg/L) and a control group for 96 h. After that, blood and plasma factors were measured in blood samples. Mortality rate and fish behavioral were recorded during the experiments. LC50, LOEC, NOEC and MATC were calculated by Probit software according to mortality rate in any treatment.
Results: In the early period of the experiment, fish in toxic media were jumping in water and showed high movements. Then after, the behavior such as breathing in surface, loss of balance, weakness and finally death were observed in the different treatments. LC50 value and 95% confidence limit of glyphosate in 24, 48, 72 and 96 h were 36.09, 30.46, 21.78 and 20.05 mg/L for common carp, respectively. The LOEC, NOEC and MATC were calculated as 6.66, 4.73 and 2.005 mg/L. The Significant changes that were observed in blood factors included RBC, WBC, Hct, Hb and plasma factors such as total protein, glucose, cortisol and other factors when the pesticide concentration was increased (p<0.05).
Conclusion: Results showed that glyphosate had a high effect on common carp and it its physiology and immune system. Haematological parameters may be used as an environmental health diagnostic test and stress indicator for agriculture pesticides in aquatic ecosystems.
Maryam Delfani, Maryam Mohammadi Rouzbahani, Nasrin Choobkar, Noushin Salimi,
Volume 15, Issue 3 (12-2022)
Abstract
Background and Objective: Today, oil and gas industries are important resources for obtaining energy and income. During the production of oil and gas, very complex compounds that include organic and inorganic substances, petroleum hydrocarbons and aromatic and aliphatic compounds are transferred to the environment. benzene, toluene, ethylbenzene and xylenes, which are called BTEX for short, are very important monoaromatic pollutants. Therefore, the present study was conducted to investigate the effect of exposure to BTEX on some clinical factors of the exposed people in the oil warehouse and office workers in Kermanshah National Oil Products Distribution Company.
Materials and Methods: This cross-sectional study was conducted in 2018. The samples of this study include the personnel working in the oil warehouse and the office staff of the National Oil Products Distribution Company in Kermanshah. After completing the demographic information of the participants in terms of the inclusion criteria and after collecting the results of the physiological tests of the employees, SPSS19 software was used for statistical analysis.
Results: The results of the present study showed that the spirometric indices decreased in the workers exposed to BTEX compounds, but it was not statistically significant. Also, the number of red blood cells (6.73%) was significantly lower in non-administrative employees, as well as red white blood cells (6.61%), SGOT (10.14%) and SGPT (5.09%) in non-administrative employees. It has been more.
Conclusion: The number of platelets was higher in office workers. Considering the dangers of BTEX pollution for human health, it is recommended to use preventive measures such as using a special mask and a shift work system.
