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Showing 3 results for Cell Immobilization
Decolorization of Remazol Briliant Blue Royal by Ganoderma Sp. Immobilized in Sodium Alginate
Kazem Naddafi, Mehran Mohammadian Fazli, Ali Reza Mesdaghinia, Simin Nasseri, Mahnaz Mazaheri Assadi, Masoud Yunesian,
Volume 5, Issue 2 (10-2012)
Abstract
MicrosoftInternetExplorer4 Background and Objectives: Environmental pollution and health risks of dyestuffs extensively are caused by many industries. Nonbiodegradability of dyes is important so that different methods are studied for removing them. The use of white rot fungi is promising technique in this regard. Therefore, objective of this work is to investigate Rimazol Brilliant Blue Royal decolorization by immobilized Ganoderma sp. in sodium alginate from aqueous solution.
Material and Methods: This is an experimental study. First, the nutritional, environmental, and operational conditions of decolorization process were optimized. Then, efficiency of immobilized fungal cells was investigated. Experimental designs were provided using fractional factorial methods and quadratic model was fitted on decolorization data by MiniTab software.
Results: Our findings showed that type and concentration of carbon source, temperature, and pH were the most important factors affecting decolorization and statistically significant. Optimal conditions to 95.3 percent color removal were: glycerol as carbon source at 19.14 g/L temperature, 27 oC and initial pH, 6.26. Moreover, decolorization efficiency increased from 75 percent up to 95 percent by improving process and fungal immobilization.
Conclusion: Ganoderma fungus has suitable potential to decolorization. Besides, optimization and cell immobilization can improve its capability. Application of experimental design to research methodology is important because of decreasing in experiments and saving resources. It is suggested to use these potentials in environmental pollution control.


Application of immobilized microbial cells in biological treatment of contaminated effluent with aromatic compounds: a review article
A Partovinia, Z Shamsollahi,
Volume 12, Issue 2 (9-2019)
Abstract
Background and Objective: Bioremediation of contaminants by living microorganisms is a favorable method for elimination or degradation of pollutants to less harmful substances. In the recent decades, cell immobilization technique has been applied to improve biodegradation efficiency and also overcome to free cells disadvantages. The purpose of this review article is to investigate the application of cell immobilization technology with emphsize on polymeric matrices in the aromatic pollutants removal in laboratory scale (shake flask and bioreactor). Also, the performance of free and immobilized cells has been compared in various environmental conditions.
Materials and Methods: In this survey 401, 78, 49, 1450, 0 and 0 relevant articles were found on Scopus, Web of Science, PubMed, Google scholar, SID and Magiran databases, respectively by using keywords such as “Cell immobilization”, “Biodegradation” and “Aromatic. Out of 1978 articles, 1167 articles were excluded from the study. Finally, 811 articles were further reviewed.
Results: According to the previous studies, removal of contaminants by immobilized cells using appropriate matrices is higher than freely suspended cell systems. Also, among the cell immobilization systems, cell entrapment in the polymeric carriers is the most widely used method for the bioremediation of aromatic contaminants.
Conclusion: Regarding the superiority of immobilized cells in comparison with free cells specially in harsh environments, the reuse of immobilized cells and their application in bioreactors as well as their scale up potential, development and application of these methods can be considered by researchers for wastewater treatment in our country.
 

Biodegradation of crude oil by immobilized microbial cells in alginate beads produced by electrospraying technique
E Khanpour-Alikelayeh, A Partovinia, A Talebi, H Kermanian,
Volume 12, Issue 4 (2-2020)
Abstract
Background and Objective: Petroleum compounds are major contributors to aquatic environmental pollution. In recent years, biological treatments as environmental-friendly and cost-effective techniques have been used alongside the various physico-chemical methods. Microbial cell immobilization in hydrogel carriers has been the focus of researchers due to various advantages such as ease of microbial species control, non-direct exposure of pollutants to the cells, increasing cell resistance during different types of stresses and reusability. The main goals of this study were introduction to electrospraying technique in order to size reduction of alginate beads and comparison of heavy crude oil biodegradation using an isolated strain of Bacillus licheniformis in free and immobilized cells.
Materials and Methods: The oil-degrading strain was isolated from oil-polluted site on Kharg Island. Microbial cells were examined in both free and immobilized systems under different conditions (pH=5,7) and initial crude oil concentration (1500,3500 ppm). Electrospraying technique was used for alginate beads production. Residual crude oil content was analyzed by gas chromatograph and gravimetrically method.
Results: The maximum oil removal (61%) was obtained for the immobilized cells at a concentration of 3500 ppm in neutral medium. Overall, according to the results, after the 14th day, the biodegradation through the immobilized cells was significantly (p<0.05) higher than the free cells. Moreover, the cell immobilization caused the microorganisms to be more resistant to the harsh environments.
Conclusion: This study showed that the immobilized microbial cell system has a great potential for oil wastewater treatment. The electrospraying technique can be used to overcome to the mass transfer limitations.

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