Showing 3 results for Cyprinus Carpio
S.r Olyaei, A.r Riahi Bakhtiari, I Sharifpour,
Volume 8, Issue 2 (8-2015)
Abstract
Background and Objectives: Over the past few decades, the entry of pollutants, especially PAHs compounds in natural ecosystems such as lakes and open water for aquatic organisms has created potential environmental hazards. This study aimed to investigate the extent and pattern of bioaccumulation of pyrene oil pollution in muscle, gill, and liver of common carp (Cyprinus carpio) cultured in vitro. Materials and Methods: thirty fishes with mean weight of 140±10g were exposed to 10, 50, and 100 µg/l pyrene. At the end of the 35 days trial period, fish were sampled in order to study the pyrene accumulation in different tissues. Preparing process of samples include soaping, extracting, and two-phase chromatography and finally injecting samples to the gas chromatography-mass spectrometry. Results: Comparison between bioaccumulation of pyrene in muscle, gill, and liver tissues of cyprinus carpio in 10, 50, and 100 µg/l doses showed significant differences. Maximum average accumulation was in liver (dose100) with 685.67 ± 78.5 and the minimum was in muscle in dose 10 with 0.52 ±. 0.13 pyrene’s accumulation trend was the same in all studied doses: liver>gill>muscle. Conclusion: In the present study, pyrene accumulated in fatty tissue, especially liver-acceptor of all of the oil pollutants- was far more than of gills and muscle.
N Naqshband, M Askari Hesni,
Volume 10, Issue 2 (9-2017)
Abstract
Background and Objective: Glyphosate is a non-selective, systemic herbicide that can control most plants, but it has harmful effects on organisms, especially aquatic animals. Static bioassays were made to determine acute toxicity of glyphosate and its effects on haematological parameters and behavior changes of common carp (Cyprinus carpio).
Materials and Methods: Common carp were exposed to different concentrations of glyphosate (5, 10, 20, 30 and 40 mg/L) and a control group for 96 h. After that, blood and plasma factors were measured in blood samples. Mortality rate and fish behavioral were recorded during the experiments. LC50, LOEC, NOEC and MATC were calculated by Probit software according to mortality rate in any treatment.
Results: In the early period of the experiment, fish in toxic media were jumping in water and showed high movements. Then after, the behavior such as breathing in surface, loss of balance, weakness and finally death were observed in the different treatments. LC50 value and 95% confidence limit of glyphosate in 24, 48, 72 and 96 h were 36.09, 30.46, 21.78 and 20.05 mg/L for common carp, respectively. The LOEC, NOEC and MATC were calculated as 6.66, 4.73 and 2.005 mg/L. The Significant changes that were observed in blood factors included RBC, WBC, Hct, Hb and plasma factors such as total protein, glucose, cortisol and other factors when the pesticide concentration was increased (p<0.05).
Conclusion: Results showed that glyphosate had a high effect on common carp and it its physiology and immune system. Haematological parameters may be used as an environmental health diagnostic test and stress indicator for agriculture pesticides in aquatic ecosystems.
S Bita, M Mesbah, A Shahryari, M Ghorbanpoor Najafabadi,
Volume 10, Issue 3 (12-2017)
Abstract
Background and Objective: Nanoparticles are already widely used in technology, medicine and consumer products, but there are limited data on their effects on the aquatic environments. The aim of this study was to evaluate the response of antioxidant defense system in common carp gills exposed to silver nanoparticles, which are produced by bioreduction method.
Materials and Methods: Common carp fish were exposed to the silver nanoparticles at concentrations of 0.11, 1.13 and 5.67 mg/L for 14 days. A treatment without silver was considered as a control. After sampling, 1 g of gill was weighed and homogenized in 5 mL phosphate buffer. The homogenized samples were analyzed for measuring the activity of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), total antioxidant capacity (TAC) and malondialdehyde (MDA).
Results: CAT activity in gill at the concentrations of 1.13 and 5.67 mg/L AgNPs was significantly reduced compared to that of the control (p<0.05). When the activities of GSH and TAC of different treatments and the control were compared, no significant difference (p>0.05) was observed. Activity of SOD and MDA of all treatments sampled at different days was fluctuated, that is, it was either increased or decreased. However, superoxide dismutase activity was significantly higher in exposure to concentrations of 5.67 mg/L and 1.13 mg/L.
Conclusion: According to the results, fish gills are one of the most susceptible organs of oxidative damage in exposure to silver nanoparticles. This can affect the health of common carp and thus increase the risk of disease in the fish.
