Fahim Amini, Masoud Yunesian, Mohammad Hadi Dehghani, Nima Hosseni Jazani, Ramin Nabizadeh Nodehi, Maasoumeh Moghaddam Arjomandi,
Volume 5, Issue 1 (4-2012)
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Background and Objectives: Nosocomial infection is the cause of deaths, morbidity, higher costs
and increased length of stay in hospitals. Correct and appropriate use of
antiseptic and disinfectants play an important role in reducing infections. In
this study the efficacy of antiseptics on bacteria causing hospital infections
has been studied.
Materials and Methods: This study was conducted in the laboratory of Imam Khomeini Hospital of Uremia.
In this study the Antimicrobial activity of Descocid, Korsolex basic, Mikrobac
forte and persidin 1% was studied against bacteria causing hospital infections
such as Enterobacter aeruginosa 1221 (NCTC 10006), Staphylococcus epidermidis
(PTCC: 1435 (Cip81.55) and Pseudomonas aeruginosa Strain PAO1. Sensitivities of
bacteria were determined by Minimum inhibitory Concentration (MIC) and Minimum
bactericidal Concentration (MBC) antiseptics. In the second stage, the
concentration of antiseptics was prepared according to the manufacturer's
suggested protocol and the effect of antimicrobial agents were studied at the
certain concentration and contact time.
Result: All
disinfectants (Descocid, Korsolex basic, Mikrobac forte) concentration and
contact time, Accordance with the manufacturer's brochure, had inhibitory
effect on all bacteria. That this is consistent with the manufacturer's
brochure. Persidin one percent in concentration of from 2 and 4 V/V % and
exposure time 5 minutes could not inhibit the growth of bacterial. But at
concentrations of 10 and 20% respectively 15 and 30 minutes exposure time, all
three types of bacteria can be inhibited, which is consistent with the
manufacturer's claims.
Conclusion: In this
study, the efficacy of antiseptics was determined with the Micro-dilution
method recommended by the NCCLS. Korsolex basic, weakest antiseptics (the
highest MIC) for the inhibition of three bacteria was determined. But Between
all four antiseptics (according to manufacturer concentration), Only one percent
Percidine 2 and 4 V/V % in consumer
dilution and 5 minutes exposure time failed to inhibit the growth of
Pseudomonas aeruginosa, Staphylococcus epidermidis and Enterobacter aeruginosa.
