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Showing 4 results for Fecal Coliform
Identification of Total and Fecal Coliforms and Heterotrophic to Microbiological Method and E.coli O157:H7 to Immunological, and RealTime PCR Methodsin IsfahanWaterTreatment Plant
P Atabakhsh, M.m Amin, H Mortazavi, M Yaran, A Akhavan Sepahi, A Nouhi, M Jalali,
Volume 3, Issue 3 (10-2010)
Abstract

Backgrounds and Objectives:Total and Fecal coliforms (TC and FC), heterotrophic plate count (HPC), were counted by microbiological method and E.coli O157:H7 were detected through immunological and Real time PCRmethods inwater intake and all of units of Isfahanwater treatment plant (IWTP).
Materials and Methods: The microbial profile including TC, FC, and HPC, were monitored and turbidity and total organic carbon were analyzed in 8 locations of water intake, and unit operation and processes of IWTP, including, inlet, sedimentation, ozonation, and filtration and finished water. Immunological method through anti-serum kits and molecular method of RT-PCR were used to detect E.coli O157:H7 in the 8 locations and also the sludge of the sedimentation basin and filters backwash water of IWTP.
Results: Survival of E.coli O157:H7 in sludge sample of sedimentation basin was indicated by formation of agglutination particles in immunological method and through indicator probes in the RT-PCR method. However, E.coli O157:H7 was not detected in water samples of other units of IWTP. The removal percent of TC, FC, and HPC were: 59.5, 49, and 54.8 % in sedimentation basin 66, 45.8, and 57 % in ozonation: 98.8, 98, and 78.8 in the filtration and 96, 100, 91% in disinfection, respectively.
Conclusion: This study approved the existence of the pathogenic coliform, E.coli O157:H7 in the
sludge of sedimentation basin. Absent of E.coli O157:H7 in the finished water indicates that the existing units of IWTP could eliminate these pathogenic bacteria, before reaching the final units of the plant, including the filters and disinfection.


Study of soil bacterial and crop quality irrigated with treated municipal wastewater
Afsaneh Alinezhadian, Ahmad Karimi, Jahangard Mohammadi, Farzaneh Nikookhah, Mathias Niuman. Anderson,
Volume 6, Issue 3 (12-2013)
Abstract
Background and Objectives: In arid and semi-arid regions, wastewater reuse has become an important element in agriculture. However, irrigation with this resource can be either beneficial or harmful, depending on the wastewater characteristics. The aim of this research was to investigate the soil bacterial and crops quality irrigated with treated wastewater. Material and Methods: This research was conducted on a maize field near the wastewater treatment plant in Shahr-e-kord in summer,2011. Plots were arranged in a randomized complete block design in 3 replications and 2 treatments, well water (W1) with fertilizer and effluent (W2). Results: At the end of growth season, soil samples were collected from depth of 0-5 and 5-15 cm and plant samples consisting of old and new leaves and seeds were collected for bacteriological analysis. According to bacteriological analysis, total number of positive lactose bacteria, total and fecal coliforms in depth of 0-5 cm was 42% more than depth of 5-15 cm. In the case of old leaves, total number of coliform and fecal coliform was 88 and 40 MPN/100 mL respectively. Moreover, for new leaves, it was 38 and 2 MPN/100 ml respectively. Conclusion: According the results, number of indicator bacteria in soil is decreased (about 35%) by passing time.
Evaluation of Microbial Quality in Biosolids Generated from Municipal Wastewater Treatment Plants
B Ghoreishi, M Shaker Khatibi, H Aslani, A Dolatkhah, A Abdoli Seilabi, M Mosaferi,
Volume 9, Issue 1 (6-2016)
Abstract

Background and Objectives: Qualitative evaluation of sewage sludge before any kind of application is essential. The present study was aimed to investigate Total coliform, Fecal coliform and Salmonella in sewage sludge produced at wastewater treatment plants in Azerbaijan Province, Iran.

Materials and Methods: Nine wastewater treatment plants were chosen in East Azerbaijan Province, and their sludge from drying bed was studied. Total coliforms, thermo-tolerant coliforms, and Salmonella spp., were surveyed during winter time, 2015. Total and thermos-tolerant coliforms were enumerated by EPA method 1680 and salmonella was counted using EPA method 1682.  

Results: In the case of total coliform, sludge sample from Jolfa with 1.82×106 MPN/g showed the highest contamination, while Sarab showed lowest fecal coliform count with 2.02×103 MPN/g. As in the case for fecal coliform, the bacteria count for thermo-tolerant coliforms was higher in Jolfa than other cities; on the other hand, Ahar with no fecal coliform count or less than 2.2 showed the minimum contamination rate to fecal coliforms. In case of Salmonella spp., sludge samples from Ahar and Bostan Abad did not show any salmonella. While sludge sample from Tabriz wastewater treatment plant was determined as the most contaminant sludge with bacteria count equal to 84 per  g. Moreover, sludge sample from Sarab wastewater treatment plant showed the least contamination rate, and bacteria count was 6 per  g.

Conclusion: From the stand point of microbial quality, all sludge samples met class B standards set by USEPA, while none of them could provide class A standards. Thus, special precautions must be taken in case of soil amendments by the sludge produced from wastewater treatment plants. 


Analytical assessment of Mahabadchai river water quality using Iran water quality index
Reza Kheiri Soltan Ahmadi, Habib Nazarnejad, Farrokh Asadzadeh,
Volume 14, Issue 4 (3-2022)
Abstract
Background and Objective: With the industrialization of communities, population increase and use of surface water, river pollution has been increased by agricultural, industrial pollutants and urban wastewater. Therefore, investigation of river pollution for regional and environmental planning is of great importance. To evaluate surface water pollution, a number of surface water quality indices have been investigated.
Materials and Methods: Iran water quality index for surface water resources (IRWQISC) approach was used to evaluate the water quality of the Mahabadchai river, Iran. Sampling was carried out along the Mahabadchai river based on some criteria such as approximately to drainage areas for landfills/domestic and agricultural effluents.
Results: Pearson correlation coefficient between physical, chemical and microbial parameters of water showed that fecal coliform, BOD, COD, nitrate, ammonium, phosphate, turbidity and total hardness had a significant positive relationship with each other at 99% confidence. According to this index, upstream samples of the river are classified as good, mid-stations relatively good to moderate category, and downstream samples of the river are classified as relatively bad.
Conclusion: Based on the relationship between each variable, fecal coliform, BOD, COD, ammonium, and turbidity were more effective in determining the IRWQISC. Most of the examined variables showed low concentrations in upstream areas of the river while their concentration gradually increased along the river to downstream areas, especially close to urban and industrial districts.
 

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