Mahdis Mosayebi, Habiballah Mirzaee,
Volume 6, Issue 4 (3-2014)
Abstract
Background and Objective: Rice is one of the most consumed food items that is exposed to mycotoxins and heavy metals contamination. Due to the accumulation of heavy metals in the tissues of human body and its side effects on the body and being carcinogenic and mutagenic mycotoxin, the present study was conducted to determine contamination of rice imported in Golestan Province. Materials and Methods: We collected 80 samples of imported rice from India and Pakistan on the Inche boron border in Golestan province between January 2011 and August 2012. We analyzed each sample for the amount of mycotoxins (aflatoxins B1, B2, G1, G2 and total aflatoxins, ochratoxin A, zearalenone and dexynivalenol) and heavy metals (lead, arsenic, and cadmium). We used HPLC to measure mycotoxins and immunoaffinity column (Dexynivalenol purification was conducted using DONSPE column) for purifying. Hydride generation atomic absorption based optical spectroscopy technique was used for determining arsenic. Graphite furnace atomic absorption according AOAC.986.15 was used to determine lead and cadmium. Results: We found that the average level of aflatoxin B1, B2, ochratoxin A, and total aflatoxin were 0.93, 0.036, 0.033 and 0.96 ng/g respectively. Aflatoxin G1, G2 and dexynivalenol was not observed in any of the samples. Average concentrations of lead, arsenic, and cadmium in the samples were 0.067, 0.007, 0.024 mg/kg respectively. Conclusions: All samples were within the accepted maximum limits of Iran National Standards for heavy metals and mycotoxins in rice.
R Farahmandfar, S Rashidaei Abandansari , E Maghsoudlou, M Asnaashari,
Volume 11, Issue 1 (6-2018)
Abstract
Background and Objective: Wheat is one of the most important cereals due to the supply of much of the flour used in bread making in most countries, such as Iran. Wheat contamination with mycotoxins is subject to different production and maintenance conditions. As a carcinogen and mutagen, monitoring the amount of mycotoxins in imported wheat and prevention of the entry of low quality wheat to the food chain are essential. Therefore, this study was conducted to determine the contamination of the wheat imported to Mazandaran province.
Materials and Methods: Samples of the wheat imported from Caspian Sea countries were evaluated for moisture content and mycotoxins contamination (ochratoxin A, zearalenone and deoxynivalenol). Mycotoxins contamination was determined by HPLC method via purifying with immunoaffinity column for ochratoxin A and zearalenone, and DONSPE column for deoxynivalenol.
Results: The results of this study showed that the average of ochratoxin A, zearalenone, and deoxynivalenol in the samples were 2.24, 133.50 and 181.66 ng/g, respectively.
Conclusion: All the samples were within the acceptable level for mycotoxin according to the permissible limits of Iran National Standard. Additionally, a direct relation between deoxynivalenol and moisture content of the samples (-0.092) was not observed and the correlation between moisture content and ochratoxin A and zearalenone was 0.104 and 0.168, respectively.
