Iranian Journal of

Background and Objective: Food-borne diseases are among the most important public health problems. Among them, Salmonella is one of the most important food pathogens. Different Salmonella serotypes enter body through food and causes food infections in consumers. One of the most important evaluation criteria for health is to determine the microbial load of food.
Materials and Methods: In this study, 245 shrimp samples supplied in Khuzestan province were taken randomly. The samples kept on ice were transferred to the laboratory and immediately tested microbiologically.     
Results:  In this study, in 50.2 percent of the samples, the average number of bacteria per mL of the rinse water used to rinse the shrimps was 2200 bacteria, in 29.8 percent of the samples, it was 13600 bacteria and in 20.0 percent of the samples, the average number of bacteria per mL of the rinse water, it was 36700 bacteria. Based on these results, the average number of bacteria in the total samples was 20000 bacteria per mL rinse water. Out of the total samples assessed 33 samples were positive and 212 were negative for Salmonella, showing a 13.4 percent prevalence of Salmonella contamination in the shrimp production in Khuzestan Province.
Conclusion: Due to the microbial load and Salmonella contamination in shrimp supply in the province of Khuzestan raw or undercooked consumption of shrimps can increase the possibility of problems for consumers.
 

Background and Objective: A large amount of waste is generated from shrimp farming, primarily consisting of shrimp heads and shells. These wastes are often discharged into aquatic environments, where they act as pollutants and threaten the survival of other aquatic organisms. Therefore, reusing shrimp waste can help reduce water pollution and mitigate its environmental impact.
Material and Methods: In this study, carotenoids from shrimp waste were extracted using three methods: acidic, enzymatic, and alkaline. The amount of carotenoids was quantified for each extraction method. Their antioxidant effects on crude sunflower oil, in the presence of copper sulfate, were then evaluated using the TBARS assay. In this method, malondialdehyde levels are measured as an indicator of lipid oxidation. For this purpose, equal amounts of the extracted carotenoids were added to crude sunflower oil along with copper sulfate and placed on a shaker for 24 hours. Subsequently, the malondialdehyde content was measured.
Results: The highest carotenoid yield was obtained using the alkaline extraction method. In the TBARS assay, the alkaline method also demonstrated the greatest inhibition of lipid peroxidation in the presence of copper sulfate.
Conclusion:  It can be concluded that the alkaline method is a simple and cost-effective approach for extracting carotenoids from shrimp waste. These carotenoids are bioactive and valuable compounds with potential applications in the pharmaceutical and food industries.