Background and Objectives: Over the past few decades, the entry of pollutants, especially PAHs compounds in natural ecosystems such as lakes and open water for aquatic organisms has created potential environmental hazards. This study aimed to investigate the extent and pattern of bioaccumulation of pyrene oil pollution in muscle, gill, and liver of common carp (Cyprinus carpio) cultured in vitro. Materials and Methods: thirty fishes with mean weight of 140±10g were exposed to 10, 50, and 100 µg/l pyrene. At the end of the 35 days trial period, fish were sampled in order to study the pyrene accumulation in different tissues. Preparing process of samples include soaping, extracting, and two-phase chromatography and finally injecting samples to the gas chromatography-mass spectrometry. Results: Comparison between bioaccumulation of pyrene in muscle, gill, and liver tissues of cyprinus carpio in 10, 50, and 100 µg/l doses showed significant differences. Maximum average accumulation was in liver (dose100) with 685.67 ± 78.5 and the minimum was in muscle in dose 10 with 0.52 ±. 0.13 pyrene’s accumulation trend was the same in all studied doses: liver>gill>muscle. Conclusion: In the present study, pyrene accumulated in fatty tissue, especially liver-acceptor of all of the oil pollutants- was far more than of gills and muscle.
