Showing 4 results for Seroepidemiology
V Torabi, M Mohebali, Ghh Edrissian, H Keshavarz, M Mohajeri, M Hajjaran, B Akhoundi, Aa Sanati, Z Zarei, A Delshad,
Volume 4, Issue 3 (3-2009)
Abstract
Background & Objectives: This study aimed to determine of the seroprevalence of visceral leishmaniasis to design a prevention and control program in Bojnoord district.
Methods: This was a descriptive and cross sectional study with multistage random sampling method. The study was carried out on children up to 12 years old and 10% of adult’s population in 8 rural areas of Bojnoord district in 2007. In total, 1608 blood samples were collected to detect anti-Leishmania antibodies. All the samples were tested by direct agglutination test (DAT). In order to determine Leishmania species, necropsy was performed on four suspected dogs in the studied areas. The species of Leishmania was determined by RAPD-PCR and PCR-RFLP methods using to amplify the ribosomal internal transcribed spacer 1 (ITS1).
Results: Thirty and eight (2.36%) out of 1608 collected blood samples had anti-Leishmania antibodies at titer 1:800 and nine (0.56%) were just positive at 1:3200 by DAT. There was no statistically significant difference between female and male seroprevalence (p<0/05). The seoprevalence in children <=12 years old compared to individuals greater than 12 years old did not show any statistically significant (p<0/05). Amastigotes were observed in all 4 necropsied dogs. The species of Leishmania isolated from 2 dogs, was determined as L.Infantum. Their ITS1 sequences were registered with Accession numbers EU810776 and EU810777 in NCBI.
Conclusions: These findings showed that visceral leishmaniasis has been circulated with low endemicity in Bojnoord district. Therefore an appropriate monitoring system is needed for health services in this area.
D Tayyebi, M Tabatabaii, M Rahsaz, S Sharifi, M Shariati, I Sohrabi,
Volume 5, Issue 3 (12-2009)
Abstract
Background & Objectives: Cytomegalovirus (CMV) is a one the cause of congenital illness and disability, including hearing loss and mental retardation. CMV is a herpesvirus which establishes a persistent life-long infection. Since the prevalence of antibodies against the virus varies in different geographical areas in the world, we decided to identify the seroepidemiology of CMV infection.
Methods: A descriptive study was conducted among 360 volunteer asymptomatic healthy female students in Islamic Azad University of Kazeroun. The men age of subjects was 20.8 years. For serological studies five (ml) of blood sample was collected and the serum was isolated by centrifugation. Enzyme linked immunosorbent assay (ELISA) was used for determination of immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies of CMV. Finally the results were analyzed by statistical methods.
Results: Overall, CMV-IgG antibody was positive in 340 persons (94.4%) out of 360 subjects and they have a previous infection. However, Only four persons (1.1 %) were determined to have IgM antibody. Also we couldn’t find any significant difference concerning their fields of study and their residency (p>0.05).
Conclusions: With considering the high rat of CMV infection rate in young female, it is suggested to have appropriate preventive programs for women particular in susceptible groups.
Y Garedaghi, Sr Bahavarnia,
Volume 7, Issue 2 (9-2011)
Abstract
Background & Objectives: the aim of this study is seroepidemiological survey of human Hydatidosis by ELISA method in East-Azarbaijan province.
Methods: Totally 1500 serum samples were collected from patients referred to different health centers in 8 cities of East-Azarbaijan province. All the sera were examined using ELISA test.
Results: Nineteen cases (1.28%) were positive for human hydatidosis in East-Azarbaijan province using ELISA test. From these cases, 8 cases (0.93%) were from urban areas and 11 cases (1.8%) were from rural areas. 1.76% of females and 0.83% of males were positive and age group 30-40 years old had the highest rate of positivity (1.66%). There were no statistically significant different between gender and age groups and residency area.
Conclusion: According to results of this study the highest rate of positivity was seen in Sarab city (2.17%). The possible reason of this difference could be to live the highest rate of rural population of East-Azarbaijan province dwelling in this city.
M Mohammadkhani , H Sharifi, H Rashidi, Ar Nabipour, M Jahanshahi,
Volume 10, Issue 4 (3-2015)
Abstract
Background and Objectives: Brucellosis is a zoonosis disease that is important in humans and animals. Since the disease is important in ranchers and veterinarians, we conducted this study to estimate the seroprevalence of the infection in these groups in Kerman.
Methods: In this descriptive and analytical cross-sectional study, 187 industrial and semi-industrial dairy personnel and veterinary network staff of Kerman in 2012 were recruited. Rose Bengal, Wright and 2-Mercaptoethanol were done on serum samples. Demographic and occupational characteristics of the participants were recorded. Data was analyzed using descriptive statistics and Poisson regression via the Stata software. Results: The prevalence of brucellosis was 3.2%. In this study, the history of brucellosis in the family, working in the veterinary network, and working in semi-industrial versus industrial dairy farms were the risk factors of the disease.
Conclusion: Although the prevalence of the infection was lower than other studies, it was still considerable in these groups therefore, it is necessary to improve preventive measures in these groups.
