Showing 6 results for Miraminmohammadi
Farzaneh Zarrinkar, Ali Khamesipour, Akram Miraminmohammadi, Ebrahim Eskandari, Mahmoud Nateghi Rostami, Esmaeil Fallah,
Volume 3, Issue 3 (9-2012)
Abstract
Background and Aim: Leishmanization (LZ) is an effective tool to prevent
cutaneous leishmaniasis. Standardization of Leishmania is
the main drawback of LZ. The aim of this study was to assess the effect of
various preservatives on the infectivity of Leishmania.Methods: L.major harvested
at different stages of growth logarithmic, early and late stationary phases
were frozen using various preservatives of saccharose, glycerol, trehalose,
glucose, sorbitol, and dimethyl sulfoxide (DMSO). The harvested parasites were
inoculated into BALB/c mice before and after freezing. The infectivity of the
parasites was checked. IFA test was used to assess the rate of metacyclic
parasite.
Results: The ratio of
live Leishmania in different growth stages and various
preservatives were 89.0% to 98.2%. The lesion development in groups of mice
which received Leishmania in sacarose + glycerol or DMSO was started from 3rd
week and at 5th week all the mice showed lesion. The group of mice
which were inoculated with early or late stationary phases in saccharose +
glucose, saccharose + glycerol, glycerol 15% or DMSO showed lesion from 4th
to 5th week and in 100% showed lesions at 8th week. The
rate of metacyclic parasites increases from log phase to early and late
stationary phases.
Conclusion: There was a
correlation between percent of live parasite and the rate of lesion development
in BALB/c mice. Saccharose 22.5% + Glyserol 22.5% were the most appropriate preservative to freeze L. major. IFA
test is used to detect metacyclic Leishmania. A correlation was seen between the rate of
lesion development in BALB/c mice and IFA positivity.
Ensieh Lotfali, Akram Miraminmohammadi, Mahshid Shahrzad, Ali Khamesipoor, Alireza Firooz, Azam Fattahi,
Volume 11, Issue 1 (Volume 11, No 1 2020)
Abstract
Background and Aim: The aim of the present study is rapid and precise identification of yeast species isolated from nail of patients with clinical features of onychomycosis using PCR-RFLP technique.
Methods: 50 patients with involvement of 29 fingernails and 21 toenails were enrolled in the study. Different yeast species were identified by conventional mycological. The PCR-RFLP was tested on yeast isolated and the PCR-RFLP products were separated by electrophoresis in 2% agarose gel, with DNA stain.
Results: The main causative agents were yeasts in 12 cases (24%). Candida albicans was the most commonly isolated yeast species followed by Candida parapsilosis (6%), and Candida glabrata (4 %).
Conclusion: PCR-RFLP method using ITS1-ITS4 primers and MspI restriction enzymes is a rapid, accurate and cost-effective method for specific diagnosis of the most prevalent candida spp. Its ability to detect low amounts of fungal DNA in patient samples in 6-8 hours could be useful for clinical laboratories for optimal management of these infections.
Afsarolmoluk Hadadian, Azin Ayatollahi, Akram Miraminmohammadi, Mahshid Shahrzadkavkani, Alireza Firooz, Ensieh Lotfali, Mahsa Fattahi,
Volume 12, Issue 3 (Volume 12, No 3 2021)
Abstract
Introduction: Dermatophytosis are micro-organisms which can lead to skin diseases. In rare occasions they can have atypical skin manifestations, which could result in delay in diagnosis.
Case Report: A 20-year-old Iranian boy was referred to our center with a 5-month history of circular, symmetrical, itchy crusted skin lesions on his groin. Direct microscopy, culture test and PCR-sequencing of ITS regions of the rDNA gene were conducted and showed T. indotinea as the causative agent. Based on antifungal susceptibility test oral pulse therapy with itraconazole 100 mg twice daily for 1 week combined with topical clotrimazole cream for 1 month was initiated, which cleared the lesions.
Conclusion: This case report is of significance since T. indotinea produces atypical skin lesions, as well as, multidrug resistance to antifungal agents.
Akram Miraminmohammadi, Minoo Tasbihi, Seyedebrahim Eskandari, Maryam Ahmadi,
Volume 12, Issue 4 (Volume 12, No 4 2022)
Abstract
Leishmaniasis is a neglected disease caused by different Leishmania species and transmitted by various sand flies. Iran is endemic to both visceral and cutaneous form of the diseases. The aim of this study was to investigate the epidemiological factors of leishmaniasis in patients referred to the Center for Research and Training Skin Disease and Leprosy from 1399-1400. The present study is a cross-sectional study in which every patient with suspected cutaneous leishmaniasis lesion(s) referred to the center during 1399-1400 were included. The results showed a higher number of patients with leishmaniasis are in the age group of 10 to 19 years. Among the mentioned jobs, a higher percentage was assigned to housewives in the affected women and workers in the affected men. According to the results, autumn is the most common season for cutaneous leishmaniasis. The average time from beginning to the diagnosis of leishmaniasis was 1.5-3 months and most patients showed a history of traveling to rural areas. The number of wet type lesions were higher than dry type and most of the lesions were in the hands. Most cases were reported from Isfahan province.
Seyedebrahim Eskandari, Ali Khamesipour, Alireza Firooz, Minoo Tasbihi, Akram Miraminmohammadi, Khadijeh Tavakoli, Maryam Ahmadi,
Volume 13, Issue 2 (Volume 13, No 2 2022)
Abstract
Background and Aim: Leishmaniasis is a parasitic disease transmitted by sandflies and is one of the endemic diseases of Iran. With the spread of the Covid-19 epidemic worldwide, the virus will interface with endemic infectious diseases in different regions. In this study, coronavirus disease was evaluated in patients with a history of leishmaniasis referred to the Center of Research and Training in Skin Diseases and Leprosy.
Methods: A total of 91 patients with a history of leishmaniasis were studied for possible SARS-CoV-2 infection. The number of patients referred to the Center of Research and Training in Skin Diseases and Leprosy during the coronavirus epidemic was also compared with the same period before the epidemic
Results: A total of 60% of patients with leishmaniasis
did not report coronavirus disease. Twenty-one percent of cases had a mild corona disease, 13% had a moderate and 3% had severe corona disease. Only a single death was reported in corona patients with a history of cutaneous leishmaniasis. The number of referrals before the beginning of the corona epidemic was twice as high as during the epidemic.
Conclusion: In these participants, mortality and side effects of COVID-19 were significantly less reported. However, further studies are needed to evaluate the immune system and cutaneous leishmaniasis protection against COVID-19. Various factors can be effective in reducing patients during the epidemic, including not going to medical centers, reducing the number of trips, and observance of health issues.
Minoo Tasbihi, Akram Miraminmohammadi, Ali Khamesipour, Seyedebrahim Eskandari, Alireza Firooz, Saman Ahmadnasrollahi,
Volume 14, Issue 2 (Volume 14, No 2 2023)
Abstract
Background and Aim: Cutaneous leishmaniasis is one of the most important health problems in Iran. There is still no effective vaccine available against human leishmaniasis and current treatment is based on chemotherapy. Conventional drugs are toxic and expensive, and drug resistance has caused the search for new treatments. Tofacitinib is a Janus kinase inhibitor that disturbs the signaling pathway of several cytokines in the immune system. The aim of this study was to evalute the effect of topical tofacitinib 2% ointment in healing cutaneous leishmaniasis in Balb/C mice.
Methods: This experimental study was performed using tofacitinib and glucantim drugs in 40 female Balb/C mice. The metacyclic form of promastigote of Leishmania major standard strain (MRHO/IR/75/ER) was injected into the footpad of the mice, the mice were divided into 4 treatment groups: tofacitinib, glucantim, tofacitinib-glucantim and control without treatment. The treatment period was 28 days. The footpad thickness of all groups was measured at the end of each week.
Results: After the end of the treatment in the groups treated with tofacitinib, a significant decrease in the size of lesion and the footpad thickness was observed compared to the control group without treatment (P<0.01).
Conclusion: It seems that tofacitinib ointment accelerates lesion healing in Balb/C mice and has worked similarly to standard glucantim treatment. Ointment can be a better option for leishmaniasis treatment and due to its ease of use and no need for painful injections it can be considered as an option for the treatment of patients with cutaneous leishmaniasis.
