Journal of

Background and Aim: Alopecia by reduction of hair follicles at the wound healing site is a complication of cryosurgery that creates an unpleasant appearance in the scar of healed tissue. The aim of this study was to evaluate the effect of low level laser on preventing necrosis of hair follicles and increasing hair follicles in cryosurgery induced wounds.

Methods: Fifty male Wistar rats were randomly divided into 2 main experimental and control groups (with and without low power laser) and 5 subgroups. Histopathological examination of the amount of hair follicle count and angiogenesis at intervals of 3, 7, 14, 21 and 28 days was performed in the laboratory. SPSS software was used for statistical analysis. 

Results: There was no significant difference in the  angiogenesis had significantly index between the main groups on days 14 and 21 (P>0.05), but on days 3, 7 and 28, the experimental group had significantly higher angiogenesis than the control group. Throughout the days, both groups showed a significant increase in angiogenesis (P<0.05), except for the third and seventh days in the control group, and in both groups between the 21^st and 28^th days (P>0.05). In the comparison of hair follicle index, there was an increase on all days, which was significantly higher in the experimental group than the control group (P<0.05). However, no statistical difference was observed between the third and seventh days (P>0.05).

Conclusion: The use of low-power lasers on cryosurgery induced wounds with scars and alopecia can prevent hair follicles necrosis and subsequent increase in their numbers in rats.

Background and Aim: The purpose of this study is to investigate the histopathology and immunohistochemistry of the effect of 5% Plantago labceolata. extract on open wound healing in rat skin.
 

Methods: After extracting the plant extract using the mixed method, 40 healthy male rats with an average weight of 200-250 grams were selected and randomly divided into two main groups of 20. Group 1 is the treatment group and group 2 the control group each of the main groups being divided into 4 subgroups (days 3, 7, 14,21) were divided. For the histopathological and immunohistochemical investigation of the healing process, the wounds of the mice of the subgroups in both the main groups were removed in full thickness and with a diameter of 15 mm and examined for histological studies. After sectioning, with the qualitative staining method of hematoxylin eosin and Masson's trichrome,using antibodies against transforming growth factor beta and vascular endothelial growth factor of the study groups, and the obtained information was converted into quantitative information. and was subjected to statistical analysis.
 

Results: Finding this study has shown that about 95% of women had felt at least one problem in their hands. During COVID-19 pandemic, hand washing with disinfectants were repeated more and more, furthers skin of the hands will be two times more damaged as compared to normal conditions, so the need to increase hand health training as part of general health, especially in older adults' women in advance.
 

Conclusion: The results show that it is possible to use the polar extract of Plantago Lanceolata L. plant 5% for wound healing.