Showing 5 results for Stem Cell
Mona Malekmohammadi, Hossein A. Tehrani, Nasser Aghdami,
Volume 3, Issue 1 (3-2012)
Abstract
Background and Aim: Synthesis of extracellular matrix (ECM) is a key factor in wound healing. The predominant component of ECM is hyaluronic acid (HA). Recent studies have shown that adipose derived stem cell-conditioned medium (ADSC-CM) can increase the expression and synthesis of collagen I, III and fibronectin which are the components of ECM. The purpose of this study was to investigate the effects of ADSC-CM on the expression of HA synthase and degrading genes in human dermal fibroblasts (HDF).Methods: ADSCs were isolated from three liposuction samples and their conditioned medium was collected. Skin biopsies were isolated from six patients and their fibroblasts were isolated. These cells were cultured in 100% of ADSC-CM. Expression of three HA synthase and two hyaluronidase genes were assessed with the use of real time PCR, with ELISA and final amounts of secreted HA were measured in the treated and control groups.
Results: Gene expression of two HA synthase genes HAS1 and HAS2 were upregulated in treated samples compared to the control group. The expression of one hyaluronidase gene, HYAL2, was downregulated in them. Final amount of HA was increased in the treated samples.
Conclusion: The expression and synthesis of HA can be increased by the stimulatory effect of growth factors present in ADSC-CM. Thus, secretomes of ADSCs could play a crucial role in wound healing by up-regulating the production of ECM’s components in HDFs.
Fatemeh Heidari, Abazar Yari, Maliheh Nobakht,
Volume 6, Issue 3 (10-2015)
Abstract
Emergence and spread of various diseases in the past century have been associated with many problems for the health care providers. Now a days, with advancement of technology, new methods such as cell therapy, are available, efficient and successful in some clinical areas. To use any cell, it is necessary to identify its source, so herein, we reviewed the literature of a new source of adult stem cells in the bulge of hair follicle.
Hair is composed of two parts: root and shaft. Proximal two-thirds of the hair root, called hair follicle that is surrounded by two dermal and epidermal sheaths. Epidermal sheath included inner and outer root sheath. Outer root sheath at the junction of the errector pilli muscle and sebaceous glands make the bulge that includes stem cells.
In this review we described anatomy of the hair follicle, hair growth cycle, hair follicle bulge, embryonic source of hair follicle, isolation of bulge stem cells using cell surface markers, gene expression and differentiation in bulge stem cells directing differentiation of bulge stem cells in normal skin repair, and practical advantages of bulge stem cells over other stem cells.
Leila Shahmoradi, Reza Moghadasali, Farhad Mostafaei,
Volume 10, Issue 4 (1-2020)
Abstract
Background and Aim: Mobile-based applications have the potential to increase the awareness of skin patients to their disease. The main goal is to rise clients' information about their skin disease, skin cell therapy and to provide Rooyan’s Center informations, specialists list, addresses, presentations, clips and relevant photos of the disease.
Methods: To identify the information, a researcher-made questionnaire was used. The questionnaires were validated by experts. Information system software was designed and developed using Joapp's Windows software. Finally, it was evaluated using a standard questionnaire assessing user satisfaction and program usability.
Results: Users evaluation in four axes: software performance with average (81.3%), Software display with average (78.9%), Software terminology and information with average (68.1%), the overall software capability (82.8%) and the overall mean score of 77.7% were rated as "good".
Conclusion: Treatment of this disease is necessary and important due to mental stress. Due to the inefficiency of existing treatments, complementary therapies to control the disease are on the rise. The software gives patients a broader view of their disease and has been very useful and effcient.
Hamidreza Ahmadi Ashtiani, Alireza Firooz, Hossein Rastegar, Amirhosein Askaripour,
Volume 10, Issue 4 (1-2020)
Abstract
A wound is described as any laceration in normal anatomic structure and functional integrity of the skin. Chronic wounds don’t progress through the normal wound healing process in 3 months leaving open laceration of different degrees of severity. Diabetic wound healing is an insurmountable process due to the chronic nature of diabetic wounds. For these complications, this has been a challenge in the health care system. By the appearance of regenerative medicine, advisedly stem cell-based therapies and ingredients have been gained the focus of researchers and professionals as well. As there is no definite cure for diabetic wounds and forasmuch as the appearance of regenerative medicine and cell-based therapies there is a big hope to find a definite treatment for diabetic wounds. In this article novel therapies based on stem cells were observed.
Mahdieh Ghiasi, Seyedkhalil Pestehei, Seyedamirhossein Javadi, Seyediman Seyhoun,
Volume 15, Issue 1 (5-2024)
Abstract
The skin, as the outer layer, protects the body against external factors. Wounds can negatively affect its performance. Wound healing includes three stages of inflammation, proliferation, and regeneration, which begin immediately after injury. Also, some factors such as infection, obesity and diabetes can disrupt the natural healing process that leads to chronic wounds. Various surgical and non-surgical treatments have been used to manage chronic wounds, including hyperbaric oxygen therapy, ultrasound therapy, laser therapy, and skin grafting. These treatments have advantages and disadvantages. Recently, stem cells have been used as a surgical treatment for chronic wound healing. Stem cells are highly proliferative cells that can maintain their ability to divide and regenerate for a long time. Among the different types of stem cells, MSCs have many advantages such as ease of harvest, availability, and multilineage differentiation capacity for cell therapy. In addition, they showed some properties that could be useful in the clinical application of ASCs, including angiogenesis, immune system modulation, and improved tissue regeneration. This study was conducted by collecting data from reliable scientific sources from April 1401 to January 1402 at the Research Institute of Neurosciences, Brain and Spinal Cord Injury Research Center of Tehran University of Medical Sciences. Several studies have shown that ASCs can be a suitable candidate for wound healing due to their special characteristics. The purpose of this review is to discuss the use of ASCs in wound repair and healing as a new strategy in the treatment of skin problems.
