Mona Malekmohammadi, Hossein Abdol Tehrani, Nasser Aghdami,
Volume 2, Issue 4 (12-2011)
Abstract
Skin injury caused by burns, surgery and other traumas may result in unpleasant psychological experiences and be reflected in behaviors. Extracellular matrix (ECM) is the largest component of natural skin which is gel-like and is produced by skin cells. ECM synthesis is a key factor for filling up skin wounds such as burns, leishmaniasis, chicken pox, acne, etc. ECM is composed of a variety of polysaccharides, water, and collagen proteins. Considering its weight, natural skin strength and its expandability are like steel, while it has high elasticity and compaction capacities. These characteristics are due to dual effects of main ECM molecules, which are secreted by fibroblasts and epidermal cells: 1) structural fiber proteins like: elastin, fibronectin and laminin which give strength and flexibility to ECM, and 2) proteoglycans such as dermatan sulfate and hyaluronic acid which are consisted of few glycosaminoglycan chains that branch out from a linear protein core. Proteoglycans are large and hydrated molecules which are resistant to external forces and protect underneath cells. In general, understanding the skin structure and wound healing phases can help us to design useful experiments and to conduct proper researches in this area.
Mona Malekmohammadi, Hossein A. Tehrani, Nasser Aghdami,
Volume 3, Issue 1 (3-2012)
Abstract
Background and Aim: Synthesis of extracellular matrix (ECM) is a key factor in wound healing. The predominant component of ECM is hyaluronic acid (HA). Recent studies have shown that adipose derived stem cell-conditioned medium (ADSC-CM) can increase the expression and synthesis of collagen I, III and fibronectin which are the components of ECM. The purpose of this study was to investigate the effects of ADSC-CM on the expression of HA synthase and degrading genes in human dermal fibroblasts (HDF).Methods: ADSCs were isolated from three liposuction samples and their conditioned medium was collected. Skin biopsies were isolated from six patients and their fibroblasts were isolated. These cells were cultured in 100% of ADSC-CM. Expression of three HA synthase and two hyaluronidase genes were assessed with the use of real time PCR, with ELISA and final amounts of secreted HA were measured in the treated and control groups.
Results: Gene expression of two HA synthase genes HAS1 and HAS2 were upregulated in treated samples compared to the control group. The expression of one hyaluronidase gene, HYAL2, was downregulated in them. Final amount of HA was increased in the treated samples.
Conclusion: The expression and synthesis of HA can be increased by the stimulatory effect of growth factors present in ADSC-CM. Thus, secretomes of ADSCs could play a crucial role in wound healing by up-regulating the production of ECM’s components in HDFs.
Fatemeh Yazdani Hamid,
Volume 14, Issue 1 (5-2023)
Abstract
Treatment of chronic conditions like diabetic foot ulcer (DFU) is challenging due to increased susceptibility for infection and delayed wound healing. Complexity of existing therapy, adverse effects and microbial resistance emphasizes the need of an alternative approach for the management of DFU. The increasing body of evidence associated with probiotic application in diverse disease states merits its use in wound healing and infection too. Different probiotic strains have shown their efficacy in various infections like gut infections, oral infections and urogenital infections. Experimental studies have demonstrated probiotics’ ability for gastric ulcer healing. Underlying mechanism of the above therapeutic effects of probiotics involves modulation of local and systemic immunity. The hypothesis is based on the concept that mechanism of anti-infective and ulcer healing action of probiotics will be similar in peripheral wounds and ulcers as on any other part of the body. This paper focuses on the hypothesis that topical formulation of probiotics may be effective for the treatment of diabetic foot ulcers.
Mahdieh Ghiasi, Seyedkhalil Pestehei, Seyedamirhossein Javadi, Seyediman Seyhoun,
Volume 15, Issue 1 (5-2024)
Abstract
The skin, as the outer layer, protects the body against external factors. Wounds can negatively affect its performance. Wound healing includes three stages of inflammation, proliferation, and regeneration, which begin immediately after injury. Also, some factors such as infection, obesity and diabetes can disrupt the natural healing process that leads to chronic wounds. Various surgical and non-surgical treatments have been used to manage chronic wounds, including hyperbaric oxygen therapy, ultrasound therapy, laser therapy, and skin grafting. These treatments have advantages and disadvantages. Recently, stem cells have been used as a surgical treatment for chronic wound healing. Stem cells are highly proliferative cells that can maintain their ability to divide and regenerate for a long time. Among the different types of stem cells, MSCs have many advantages such as ease of harvest, availability, and multilineage differentiation capacity for cell therapy. In addition, they showed some properties that could be useful in the clinical application of ASCs, including angiogenesis, immune system modulation, and improved tissue regeneration. This study was conducted by collecting data from reliable scientific sources from April 1401 to January 1402 at the Research Institute of Neurosciences, Brain and Spinal Cord Injury Research Center of Tehran University of Medical Sciences. Several studies have shown that ASCs can be a suitable candidate for wound healing due to their special characteristics. The purpose of this review is to discuss the use of ASCs in wound repair and healing as a new strategy in the treatment of skin problems.
