Fatemeh Hemmati, Mansour Bayat, Kumarss Amini,
Volume 34, Issue 0 (5-2021)
Abstract
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method.
Materials and Methods: For this study, 100 individuals including 50 patients and 50 healthy individuals were recruited and examined by a periodontist. After identifying sulfate-reducing bacteria by biochemical tests and specific media, the effect of aloe vera extract on them was investigated and the expression of BFR gene against housekeeping gene (16srDNA) was determined by Real time- PCR test via T-test analysis method.
Results: The data showed that 12 strains of sulfate-reducing bacteria were isolated from the samples, 5 of which had BFR gene and their gene expression was significantly reduced by aloe vera gel (P<0.05). |
Conclusion: The data of this study in proving the anti-biofilm and antibacterial effects of aloe vera extract showed that the expression of the target gene is reduced. It seems that this substance can be used as an alternative to oral hygiene chemicals.
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method.
Materials and Methods: For this study, 100 individuals including 50 patients and 50 healthy individuals were recruited and examined by a periodontist. After identifying sulfate-reducing bacteria by biochemical tests and specific media, the effect of aloe vera extract on them was investigated and the expression of BFR gene against housekeeping gene (16srDNA) was determined by Real time- PCR test via T-test analysis method.
Results: The data showed that 12 strains of sulfate-reducing bacteria were isolated from the samples, 5 of which had BFR gene and their gene expression was significantly reduced by aloe vera gel (P<0.05). |
Conclusion: The data of this study in proving the anti-biofilm and antibacterial effects of aloe vera extract showed that the expression of the target gene is reduced. It seems that this substance can be used as an alternative to oral hygiene chemicals.
Marzie Mazhari, Ali Habibikia, Ali Badfar, Mehdi Sabaghian, Arash Dabbaghi, Fattaneh Ghorbani Javadpour,
Volume 35, Issue 0 (5-2022)
Abstract
Background and Aims: By using the image processing capability in Cone-Beam Computed Tomography (CBCT), obtained information can be improved. The aim of the present study was to investigate the effect of sharpen image processing filter in determining the external resorption of the root apical region in single-rooted teeth due to the proximity of the impacted tooth using CBCT.
Materials and Methods: In this laboratory study, the lateral surfaces of the apical region of the root of 40 healthy extracted teeth were artificially cut by bur and the teeth were randomly divided into four groups: control, low, moderate, and high resorption. After preparing CBCT of the teeth, the samples were then processed with and without an image sharpening processing filter and examined by two observers. The results of this study were analyzed by SPSS22 and Medcalc Statistical Software version 14. The significance level was considered P≤0.05 for all the statistical tests.
Results: Specificity (100%) and sensitivity (66%>) were either complete or very high for both modes without and with the sharpen filter. The overall accuracy (0.83>) was high in both cases and no significant difference was observed between the two overall accuracies (P>0.05). Statistical analysis of both image observations by two observers showed that the overall sensitivity and accuracy in the medium and high resorption groups was much higher than the low resorption group, but the specificity in all groups was 100%.
Conclusion: The results showed that the images with a sharpening filter did not increase the detection power compared to the unfiltered images. However, as no loss of accuracy was observed with this filter, it could be used as a processing filter.
Masoud Safari, Faezeh Soltani, Shirzad Safari, Samad Darabian,
Volume 35, Issue 0 (5-2022)
Abstract
Background and Aims: Oral health is a necessity and part of general health and its importance in childhood is more than ever. The aim of this study was to investigate the knowledge, attitude, and practice of parents in Delfan city-Iran about oral health of their 6-12-year-old children in 2021.
Materials and Methods: The present study was a descriptive-analytical study that was performed on 365 parents of 6-12-year-old children in Delfan city. Convenience sampling was used. The questionnaires included four sections: demographic information, knowledge, attitude and practice about oral health. Data were entered in SPSS23. Independent t-test, analysis of variance and Pearson correlation were used to analyze the data. Significance level was considered 0.05.
Results: The mean age of the subjects was 36.5±7.1. 201 of parents were mothers. Parents' knowledge and attitude were good and their performance was fair. There was a statistically significant relationship between all three variables of knowledge, attitude, and practice (P<0.00). Also, there was a statistically significant relationship between the knowledge and education (P<0.00), mother job (P<0.04), father job (P<0.00), and economic status (P<0.00). There was a statistically significant relationship between the attitude with education (P<0.00), mother job (P<0.04), father job (P<0.01), and economic status (P<0.01), There was a statistically significant relationship between practice and education (P<0.00), mother job (P<0.01), father job (P<0.00), and economic status (P<0.00) of parents.
Conclusion: The results of this study showed that there was a significant relationship between the knowledge, attitude, and practice with education level, job and economic status. Therefore, oral health education for parents with low educational and economic level should be highly considered.
Zohreh Dalirsani, Maryam Amirchaghmaghi, Mahshid Malakouti Semnani, Mahdi Talebi, Seyed Isaac Hashemi, Mohammad Taghi Shakeri,
Volume 35, Issue 0 (5-2022)
Abstract
Background and Aims: Atypical facial pain (AFP) is a chronic disease associated with local pain in the craniofacial area. A wide range of treatments including drug therapy, psychiatric methods and open surgery are used for its treatment. Opiorphin is a pentapeptide that can block pain and also has proven antidepressant effects. The aim of this study was conducted to investigate the opiorphin concentration in AFP patients and to compare it with healthy people.
Materials and Methods: This study was performed on 30 AFP patients and 40 healthy individuals. Unstimulated saliva was collected from both groups by spitting method. HAD-A (Hamilton Anxiety Test) and HAD-D (Hamilton Depression Test) questionnaires were completed for all patients and controls. Psychological interview with the patients and psychological analysis were performed to determine anxiety and depression scores. Data analysis was done using SPSS (ver.24) statistical software
Results: The subjects of two groups were homogeneous in terms of age and sex. The mean salivary opiorphin concentrations in the case and control groups were 1.8050±0.2923 and 1.8032±0.3682 ng/ml, respectively. According to the results of t-Test, no significant difference was observed in the mean opiorphin concentration between the AFP group and control group (P=1.000). According to the Pearson's test, no significant relationship was found between the opiorphin levels and the age, sex, and anxiety or depression score in the patient and control groups (P>0.05). Also, there was no remarkable correlation between initial VAS, duration of pain and previous treatments with opiorphin levels in the AFP patients (P>0.05). No significant correlation was found between the opiorphin concentration and menopausal period in the case and control women (P>0.05).
Conclusion: The results showed that most AFP patients had some degree of anxiety and depression. However, salivary opiorphin concentration did not significantly increase in the AFP patients.
Seyed Javad Kia, Mohammad Samami, Negin Sheykhlari, Fereshteh Najar Karimi,
Volume 36, Issue 0 (5-2023)
Abstract
Background and Aims: Considering the regulatory role of vitamin D3 in the immune system, this vitamin may be able to prevent autoimmune diseases by modulating the immune system, so this study was conducted with the aim of comparison of the serum level of vitamin D3 in patients with oral lichen planus and healthy persons.
Materials and Methods: In this case-control study, the serum vitamin D3 level of 80 people, including 40 patients referred to Guilan dental school who were suffering from oral lichen planus and 40 healthy individuals referred to the laboratory for routine check-up, after matching for age and gender, was investigated. Data based on the type of variable were analyzed using independent t-tests, Mann-Whitney, Chi-square test, and Fisher's exact test using SPSS26 software at a significance level of 0.05.
Results: In this study, 80 person participated, including 29 women (72.5%) and 11 men (27.5%) in each of two groups of patients with an average age of 52.17 ± 12.12 years and healthy people with an average age of 52.10 ± 12.23 years. The average serum level of vitamin D3 in the control group was significantly higher than the case group (P=0.006). There was no statistically significant relationship between the vitamin D3 serum level and different types of oral lichen planus, gender, duration of disease, and age.
Conclusion: Considering the significant lower mean serum level of vitamin D3 in people with oral lichen planus; vitamin D3 may be effective as a supplement in the healing process of patients' lesions.
Neda Yousefi Nojookambari, Malihe Naderi, Razie Askari, Somayeh Talebi, Mana Mohammadhosseini, Sahar Shabani, Sajjad Yazdansetad,
Volume 37, Issue 0 (3-2024)
Abstract
Background and Aims: Oral bacteria play an important role in oral diseases, due to their high adaptability to different environmental areas of the mouth. In this article, an attempt was made to describe the molecular mechanisms involved in the physiological relationships of oral and dental environment bacteria and their pathogenic significance with molecular approaches.
Materials and Methods: The present systematic review was written based on the advanced and standard search of keywords including Oral bacteria, Biofilm, and Dental diseases in PubMed, Springer, Scopus, Medline, Google Scholar, Science Direct, and Web of Science databases. For this purpose, an advanced and systematic search of articles published from 1993 to 2023 was conducted to compile the present article.
Results: Bacteria in the oral cavity have nutritional adaptations that are important for living in pathogen-host relationships, including adapting to proteolytic living conditions, using the host's glycome as a nutritional interface. This includes the use of host-derived sialic acid and other glycosidases in oral bacteria. Some of these bacteria adhere to surfaces such as salivary, epithelial proteins, and glycans, which ultimately lead to biofilm formation. Bacteria living in the oral environment are constantly exposed to a wide range of stress-causing factors and oxidative stress in the biofilm.
Conclusion: Dental caries, pulp, periapical, and periodontitis diseases (including gingivitis) are among the most common bacterial diseases. Among them, tooth decay caused by the presence of Streptococcus mutans is the most common dental disease due to the production of acids from carbohydrate fermentation which is characterized by the demineralization of tooth structure.
Neda Omidpanah, Mohammad Esmi, Farzan Soltani,
Volume 37, Issue 0 (3-2024)
Abstract
Background and Aims: Multiple sclerosis (MS) is an autoimmune disease. Oral manifestations in these patients include paralysis or spasm of facial muscles, temporomandibular joint disorders (TMJ), trigeminal neuralgia, dysphagia, or dysarthria. The aim of the study was to investigate the characteristics of orofacial pain in patients with MS.
Materials and Methods: The current cross-sectional study was conducted on 250 patients with a definite diagnosis of MS referred to Iran MS Association and Iran MS Research Center. The patients were in the age group of 17 to 57 years and were selected completely randomly. Data were obtained based on two checklists and clinical examination. Finally, DC/TMD criterion was used to check TMD problems. Then the data was analyzed by SPSS version 26 software and by Fisher's Test.
Results: The prevalence of MS disease was higher in women and the average age of patients was 37 years and the average duration of MS disease among patients was 8 years. The most common pain was related to shooting and shock-like pain with a frequency of 40.4% and the lowest quality was related to dull and diffuse pain with a frequency of 5.3%. In total, 24.5% of patients with buccal-oral pain had TMD, and in this case, the DC/TMD criterion was used.
Conclusion: Temporomandibular disorders and shooting and shock-like pains are usually observed in a number of patients with MS. As a result, dentists should be aware of the presence of these pains in the patient, the pain trigger, and the drugs used by the patients, in order to provide medical services and being aware of drug interactions.
