Showing 18 results for Amini
M. Paknejad , R. Amini ,
Volume 12, Issue 2 (9 1999)
Abstract
The root trunk (RT) of molar teeth is important diagnostic parameter that has a key roll in diagnosis and treatment planning.The purpose of this study is presenting a normal index of RT in mandibular molar teeth in different buccal (B). distal (D), Lingual (Li) and mesial (M) entrances, and to compare them with each other.It is a case series study that 351 extracted molar teeth, after removal of soft tissue and debridement of calculus, evaluated with gauge (with accuracy 0.1 mm) According to our measurement:In maxilla: M-side: 3.88± 0.15 mm, B-side: 4.7± 1.7. D- side: 4.87± 0.13mm. So with P.value <0.05 M and D RT are longer than and M RT is shorter than D RT.In mandibule: B- side: 2.73± 0.11mm and L side: 4.03±- 0.13mm. So with P.value < 0.01 Li RT is longer than B RT.According to ochsenbine classification our studying teeth have medium to long RT. We prescribe for educating the crater therapy in periodontal surgery the emphasize must be focused on craters with medium and long RT.The shortness of RT in B-side of lower molars needed the most attention during osteoctomy in resective procedures. Because RT not depended to underlying factors such as age, social and economic situation,and for large amount of sample size in our study, the result could be extended in Tehran.
E. Yasini , M. Ataei , M. Amini ,
Volume 18, Issue 2 (4 2005)
Abstract
Statement of Problem: The relatively poor wear resistance of dental composite in stress bearing posterior situations has restricted wider clinical application of this restorative material.
Purpose: The aim of this study was to evaluate the three body abrasive wear of a dental composite based on a new filler (leucite: KAl Si2O6) and to compare it with the wear resistance of a composite based on commonly used Aluminium – Barium Silicate filler.
Materials and Methods: This research was an interventional study done in Iran polymer institute. Five specimens were considered in each group. All ceramic IPS Empress® (Ivoclar- Vivadent) ingots based on leucite crystals were ball milled, passed through an 800 sieve and used as filler. Experimental composites were prepared by mixing the silane- treated fillers with monomers (BisGMA and TEGDMA). Camphorquinone and amine were used as photoinitiator system. Degree of conversion of the light-cured and post-cured composites was measured using FTIR spectroscopy. The prepared pastes were inserted into plexy-glass mold and light cured (700 mw/cm2, 40 s). Then for maximum degree of conversion specimens were post- cured (120ºC, 5 hours). Three body abrasion wear testing was performed using a wear machine with 50 rpm rotational movement. In this machine, pumice (150 meshes) was used as the third body. Weight loss of specimens in each group was measured by balance after each 50 hours. After wear testing SEM examination was made specimens in each group. The data were analyzed and compared using ANOVA and Tukey HSD tests (P<0.05). Tetric Ceram was tested as commercial composite.
Results: There were significantly differences between three body abrasive wear of composites. The ranking from lowest to highest was as follows: leucite composite (19%) < Tetric Ceram (22%) < glass composite (28%). leucite composite showed the highest wear resistance value, propably due to the crystalliniy and hardness of filler.
Conclusion: Introduction of this new filler as an alternative to glass filler, significantly increases the wear resistance of the resin composites. Further investigations on mechanical properties of new composites would be beneficial in the development of new dental materials.
A. Vahid , A. Bahraminia , Mj Kharazi Fard ,
Volume 18, Issue 4 (5 2006)
Abstract
Background and Aim: Determining correct working length in root canal therapy is of great importance in successful treatment. The purpose of this study was to compare the accuracy of two electronic apex locators of fourth generation, Raypex4 and Apit7, in determining working length in vivo.
Materials and Methods: In this test evaluation study performed at Dental School of Tehran University, a total of 32 single canal vital teeth planned for extraction because of orthodontic and prosthetic reasons were selected. Working length determination by each of the two electronic apex locators was performed by two different dentists. These measurements were compared with direct visual measurement after tooth extraction. The dentists working with devices and the endodontist who determined the working length of the extracted teeth as well as the statistician, all were blind regarding the results. The data were compared using binominal distribution test with P<0.05 as the limit of significance.
Results: The accuracy of actual length determination of Raypex4 was 68.8% in the limit of ± 0.5 and 83.3% in the limit of ±1. From the actual length measurements, 75.1% were within the tolerance of +0.5 to-1. The accuracy of the working length determination by Raypex4 was 68.8% within the tolerance of ± 0.5 and 84.3% within the tolerance of ± 1. Taking the tolerance of + 0.5 to -1 mm into account, the accuracy of the instrument was about 78.8%. The accuracy of actual length determination of Apit7 was 65.7% in the limit of ±0.5 and 78.3% in the limit of ±1. Compared to the visual length determination, 78.8% of the actual length measurements were within the tolerance of +0.5 to-1. The accuracy of the working length determination was 62.6% within the tolerance of ±0.5 and 81.3% within the tolerance of ±1. Taking the tolerance of + 0.5 to -1 mm into account, the accuracy of the instrument was about 75.1%.
Conclusion: Raypex4 and Apit7 had similar accuracy in determining the actual length. However, they still can not replace the routine methods of actual length determination.
A. Monzavi , S. Zarati , T. Jafarzadeh , Mh. Amini ,
Volume 19, Issue 3 (4 2006)
Abstract
Background and Aim: Use of provisional restorations is one of the important phases in the treatment of patients who need fixed prosthesis. Some physical properties are required for these materials. The purpose of this study was the comparison of some physical properties of 4 kinds of resins: Trim, Tempron, Duralay (Aria Dent) and Acropars TRII.
Materials and Methods: In this experimental study, transverse-strength (TS), setting time (ST), polymerization shrinkage (PS) and temperature rise (TR) during polymerization were tested. In transverse strength test, 5 samples of each resin were made. TS testing was done with an Instron testing machine. ST testing (5 samples in each group) was done with Gilmore needle. In PS test, 11 cylindric samples were prepared from each resin and the shrinkage was examined with digital micrometer (up to 24 hours). In TR test, after mixing the powder and liquid of resins (8 samples in each group), the temperature rise was recorded with 10 seconds interval (up to 15 minutes). Data were analyzed with one-way ANOVA and Tukey HSD tests with p<0.05 as the limit of significance.
Results: In TS test, all samples of Trim were bent and no fracture was observed, while Tempron and Duralay showed no significant difference. Both Tempron and Duralay revealed high significant difference with Acropars TRII (P <0.01). In ST test, the difference between groups was significant (P<0.01). The lowest ST was observed in Acropars TRII (7 min), and the highest in Tempron (9.53 min). In PS test, significant difference was observed between Trim and Acropars TRII (P<0.01). PS was completed after 120 min in Tempron and Trim groups. However, PS continued to increase in other groups. In TR test, the difference between groups was significant (P<0.05). Duralay showed the highest TR and the lowest was seen in Trim.
Conclusion: In this study, Trim showed better properties than other studied groups. Acropars TRII had the lowest strength. The highest temperature rise was recorded for Duralay and Tempron, respectively.
F. Amini, Gh. Ramezanei,
Volume 22, Issue 2 (20 2009)
Abstract
Background and Aim: In treatment of posterior crossbite awareness of the effects of Haas and Hyrax rapid maxillary expanders (RME) on nasal cavity will help the clinician to select the better appliance. This study was carried out to compare the effects of Haas and Hyrax expanders on the nasal cavity of patients treated for posterior crossbite.
Materials and Methods: A clinical trial study was designed on posteroanterior (PA) cephalograms of 30 subjects to evaluate the nasal cavity width of 14 subjects (8 female & 6 male) with mean chronological age of 12± 2years who received RME with Hyrax type and 16 subjects (9 female & 7 male) with mean chronological age of 11±1.6 years who received Haas type palatal expander. Paired t-test was used to analyze the outcomes of expansion in each group. Student t-test was used to compare Haas and Hyrax groups.
Results: The mean value of screw expansion was 9±2 mm in both groups. In Hyrax group nasal cavity width
(Nc-cN) increased from 29.2 ±1.94 mm to 31.7 ±1.93mm (p= 0.001) and In Haas group it was increased from 27.75± 2.21 mm to 29.35 ± 2.26 mm(p= 0.043). When two groups were compared to each other, statistically this increase was more significant in Hyrax than in the Haas group (p=0.038).
Conclusion: In this study RME affected geometry of the nasal cavity by increasing the nasal cavity width. However In our sample, Hyrax appliance demonstrated better performance over the Haas appliance in all variables.
F. Amini, F. Soboti, M. Shariati, Z. Zoshad Haghighi,
Volume 24, Issue 2 (23 2011)
Abstract
Background and Aims: Considering the application of recycled metallic brackets in orthodontic treatments and probably more resultant metal ion release, which can cause cytotoxic side effects, this study was set up to assess the effect of recycling process of orthodontic brackets on metal ion release.
Materials and Methods: In this experimental study, 80 Discovery direct bond brackets (Dentarum, Germany) were divided into 2 groups, new and recycled brackets (n = 40), then each group was divided into two subgroups (one-week and 6-months subgroups) (n=20). The specimens were maintained in buffered solution of NaNHNo3 (pH=7) at 37°C in an incubator. After periods of one week and 6 months immersion, 0.5 mL of each solution was analyzed by spectrophotometry for the quantity of released Nickel (Ni), Chromium (Cr), and Cobalt (Co) ions. The data were analyzed using two way ANOVA analysis.
Results: After one week, the quantity of Ni, Cr, and Co ions released from new brackets were 0.65, 1.4, and 0.75 (ppb), respectively. The values for recycled brackets were 142, 2.61, and, 1.43 (ppb). These findings indicated significant difference between two groups (P<0.001). After six months, the quantity of Ni, Cr, and Co ions released from new brackets were 1200, 3.4, and 2.4 (ppb) and from recycled brackets were 2330, 6.9, and 10.8 (ppb), which showed significant difference between two groups (P<0.001).
Conclusion: Application of recycled brackets in long fixed orthodontic treatments can lead to metal ion release, specifically Nickel. Thus, the use of recycled brackets in long orthodontic treatments is not recommended.
Farimah Sardari, Marjaneh Ghavam Nasiri, Nasrin Amini, Berahman Sabzevari,
Volume 25, Issue 3 (1 2012)
Abstract
Background and Aims: The aim of this in vitro study was to assess the shear bond strength of amalgam to dentin using four dentin adhesive systems.
Materials and Methods: One hundred human molars were selected. After enamel removal, a dentin cylinder with 3 mm thickness was prepared. Eighty specimens were resorted with amalgam and four dentin adhesive systems as follows (n=20): group 1, Scotch Bond Multi-Purpose group 2, One Coat Bond group 3, PQ1 and group 4, Panavia-F. In group 5, 20 specimens were resorted with amalgam and varnish as control group. The specimens were incubated at 37°C for 24 h. The shear bond strengths were then measured by using push out method. The data were analyzed by one-way ANOVA and post hoc Duncan's tests.
Results: Mean values for bond strengths of test groups were as follows: group 1=21.03±8.9, group 2=23.47±9, group 3=13.16±8.8, group 4=20.07±8.9 and group 5=14.15±8.7 MPa±SD. One-way ANOVA showed the statistically significant difference between the bond strengths of five groups (P=0.001). Post hoc Duncan's test showed significant difference between groups 1and 3 (P=0.008), groups 1 and 5 (P=0.019), groups 2 and 5 (P=0.0008), groups 4 and 5 (P=0.042), and groups 3 and 4 (P=0.018).
Conclusion: Results of this study showed that the bond strength of amalgam to dentin using One Coat Bond as dentin adhesive system was higher than that observed in other dentin adhesive systems.
Omid Aminian, Zahra Banafsheh Alemohammad, Khosro Sadeghniiat Haghighi,
Volume 26, Issue 2 (5-2013)
Abstract
Background and Aims: Regarding the diversity of reported low- back pain among dentists in different countries and lack of control group in most of the previous studies, the purpose of this study was to compare low- back pain and related risk factors between male general dentists and pharmacists to determine the relation between dentistry and development of low back pain.
Materials and Methods: In this cross-sectional study, 261 male dentists were compared with 193 male pharmacists as a control group with Standardized Nordic Questionnaire (low back section). Subjects were at least one year in clinical practice after becoming qualified and did not suffer from connective tissue diseases and history of a traumatic event causing fracture in spinal column. The data were analyzed by Chi- square, T-test and logistic regression analyses.
Results: The prevalence of low back pain in the past 12 months was 54.8% in male dentists and 36.3% in male pharmacists (P=.001). Logistic regression analyses, adjustmenting for occupation, age, body mass index (BMI), smoking, working years and working hours per week, revealed that there was a significant association between being a dentist and having low- back pain (OR=2.54, P=0.001).
Conclusion: Dentistry as a profession in male gender is associated with low back pain, independent of age, body mass index (BMI), smoking, working years and working hours per week.
Sharareh Mousavi Zahed, Kamal Amini, Jahromi Maryam Zare ,
Volume 28, Issue 1 (5-2015)
Abstract
Background and Aims: To ensure root canal treatment success, endodontic microbiota should be efficiently reduced. Several irrigation devices have been recently introduced with the main objective of improving root canal disinfection.The purpose of this study was to evaluate the rinsing effect of RinsEndo system in reduction of enterococcus faecalis in comparison with conventional hand syringe in infected root canals.
Materials and Methods: 60 extracted single canal anterior teeth were infected with enterococcus faecalis and divided into 3 groups: RinsEndo system, conventional hand syringe and control group. The enterococcus faecalis colonies were counted in each group before and after rinsing. Data were analyzed using Variance and Kruskal Wallis test.
Results: The mean of enterococcus faecalis growth after rinsing was 3.50×103 in group with conventional syring rinsing, 2.04×103 in group with RinsEndo washing and 6.11×103 in control group. Reduction of enterococcus faecalis after rinsing was statistically significant in each group (P<0.001). The amount of reduction in number of colonies with RinsEndo and conventional syringe rinsing was higher in comparison with control group and this difference was significant (P<0.001). RinsEndo rinsing effect was statistically significantly higher in comparison to conventional syringe as well (P<0.001).
Conclusion: Rinsing with RinsEndo system was significantly more efficient in reduction of enterococcus faecalis from root canal in comparison with hand syringe washing.
Pouyan Amini Shakib, Taraneh Movahhed, Hooman Keshavarz,
Volume 28, Issue 3 (10-2015)
Abstract
Background and Aims: Choosing an appropriate style of teaching-learning by educators is a way to reach high quality education. The objective of this study conducted in Babol Dental School was to compare dental students, satisfaction of teaching theoretical general pathology course by means of a combination of lecture and work in small groups with their satisfaction of teaching the course by means of only lecture.
Materials and Methods: This study was performed in the second semester of school year 2011-12 and 2012-13. In 2012 (intervention group, response rate=92%), the teaching of theoretical general pathology course was implemented by means of a combination of lecture and work in small groups, but in 2013 (control group, response rate=81%) by means of only lecture. An anonymous self-administered questionnaire was used to assess students, satisfaction of the teaching methods. To compare the mean total score of satisfaction (ranging from 18 through 90) between the two groups, T-test was used.
Results: Comparing the mean total scores between the two groups revealed that students, satisfaction of the combined teaching method was significantly higher (P<0.001). The difference of satisfaction between genders (separately for each group) was not significant in combination group and lecture group (P=0.63 and P=0.87, respectively).
Conclusion: Regarding learning, combining lecture with other teaching methods such as work in small groups may increase health sciences students' satisfaction. In regard to teaching theoretical general pathology course, our findings could confirm this hypothesis.
Fereshteh Baghai Naini, Shiva Gandomi, Pouyan Aminishakib, Nazanin Mahdavi, Monir Moradzadeh, Mohamad Javad Kharazifard, Maedeh Ghorbanpour,
Volume 31, Issue 1 (6-2018)
Abstract
Background and Aims: In 1978 WHO (World health organization) formulated a histopathological definition for diagnosis of OLP (oral lichen planus). Previous studies showed that diagnosis of OLP with these criteria are not reproducible. In 2003 Van der Meij et al. represented new clinical and histopathologic definition for diagnosis of OLP known as modified WHO criteria. The purpose of this study was to evaluate the interobserver and intraobserver variability in the histopathological assessment of OLP based on the modified WHO criteria.
Materials and Methods: Fifty five microscopic slides which OLP and OLL (Oral Lichenoid Lesion) were recognized in their clinical differential diagnosis were given to 6 pathologists in two phases with 3 months interval. Each reviewing pathologist was asked to apply the modified WHO definition of OLP and to categorize each case as either OLP, or compatible with OLP. Then, the interobserver and intraobserver variability were assessed by calculation of repeatability coefficient and Kappa statistics.
Results: The results of this study demonstrated that interobserver variability based on modified WHO criteria was 0.77 (strong), while the intraobserver variability varied from 0.58 (moderate) to 0.82 (strong).
Conclusion: In the present study, the interobserver and intraobserver variability in the histopathologic assessment of OLP based on modified WHO criteria was stronger than previous studies which assessed WHO criteria. However, in order to propose the replacement of WHO criteria with modified WHO criteria, further studies with more cases and comparing the two methods is required.
Pooneh Mahmoudi, Kiumarss Amini, Parviz Amini,
Volume 32, Issue 1 (7-2019)
Abstract
Background and Aims: Candida is an opportunistic pathogen that causes illness in people with a defective or weakened condition. Infectious diseases (periodontal diseases) are inflammatory and malignant inflammation of the dental-gum complex, in which the growth of biofilms caused by Candida glabrata, Parapseilosis and Tropikalis is less than Candida albicans. Brown algae Sargasum is more compatible with human medicines due to having a natural origin than chemical drugs and has less side effects. The aim of the present study was to investigate the molecular characteristics of Candida species isolated from periodontal infections and the effect of Sargassum glaucescens extract on biofilm gene expression using Real-Time-PCR.
Materials and Methods: Oral samples of periodontal infection were collected from the referred patients. To isolate the candidate species, the specimens were cultured on a Sabouraud dextrose agar containing chloramphenicol (SDAc). The extracted DNA was extracted from colonies grown from Kit and Glass pearl. Grown chickens were identified by specific primers by PCR-RFLP method. In order to detect the expression of als genes in Candida isolates, RNA extraction was performed using Phenol-Chloroform and Pearl glass, and the CLSI-M27-A2 method was used to evaluate the effect of Sargasum glaucescens extract of algae.
Results: The results showed that the expression of als gene in periodontal infection is higher than other genes. Another role is als in the formation of Candida albicans biofilm. The minimum inhibitory concentration of fungal growth was 256 μg/ml by algae extract. Sargasum glaucescens reduced the expression of als gene expression by about 62% in the sample.
Conclusion: Sargasum glaucescens algae possesses specific pharmacological properties and antimicrobial and antimicrobial effects. The results of the study using Real Time PCR showed that expression of als gene in isolated studied with Sargasum algae extract was lower than untreated isolates. Thus, this indicated the positive role of treatment by sargasum glaucescens extract in reducing the expression of biofilm gene in isolates.
Pooya Jannati, Mohammad Ebrahimi Saravi, Tahmineh Bamdadian, Farhad Sobouti, Sahar Cheperli, Jamshid Yazdani Charati, Nasrin Khaki, Sahba Amini, Abbas Mesgarani,
Volume 32, Issue 4 (1-2020)
Abstract
Background and Aims: One of the most important challenges for dentists is providing a crown with appropriate marginal fit and gap. The 135-degree tooth preparation found to have some advantages such as technical ease and appropriate finish line record. Despite the advantages of 135-degree tooth preparation, scant research has been done in this area. Therefore, the aim of this study was to compare 135-degree and deep chamfer tooth preparation on the marginal fit and marginal gap of posterior metal-ceramic crowns.
Materials and Methods: Deep chamfer and 135-degree tooth preparation were performed on the two first mandibular molars with healthy coronal tissue and similar size. Impression was taken from each tooth and 30 crowns were made on each die. The sample plastered with fit checkers were pressed under the force of 40 N for 3 min in a universal testing machine. They were measured and recorded under a loop with 40x power in three points on each aspect. Data were analyzed by Wilcoxon and Kolmogorov-Smirnov tests using SPSS version 24.
Results: The mean marginal fit values in deep chamfer and 135-degree were 48.5417µm and 55.3333 µm, respectively with no statistically significant difference (P>0.05). While the mean marginal gap in deep chamfer (2.4833 µm) was significantly higher (P<0.05) than 135-degree (1.0083).
Conclusion: It can be concluded that the marginal gap by 135-degree’s tooth preparation was lower than that of deep chamfer in metal-ceramic crowns. However, no significant difference in the marginal fit between the deep chamfer and 135-degree tooth preparation was found.
Mohadese Baghdadchi, Elham Siasi Torbati, Kumars Amini,
Volume 33, Issue 1 (7-2020)
Abstract
Background and Aims: Periodontitis is one of the most common causes of damage to the gums and retaining structures of the teeth. Matrix protein, a metalloproteinase, is known as intermediate collagenase and the enzyme collagenase fibroblast, which is encoded in humans by the MMP-1 gene. The aim of this study was to investigate 1G/2G polymorphism in the MMP-1 gene and its association with the periodontal disease in the Iranian patients.
Materials and Methods: In this study, 50 patients with periodontal disease and 50 patients were selected as the control group in Kerman city. DNA was extracted from a person's blood sample using a kit. The desired primers were controlled by the NCBI site. Then, with the Tetra arms PCR technique, the desired polymorphism was multiplied. In the next step, the samples were transferred to electrophoresis gel and examined. The results were analyzed with SPSS software using T-test or Squer Chi-(X2).
Results: The percentage of 1G/1G genotype in the patients group was 8% and for the controls group was 0%. The percentage of 2G/2G genotype for the patients group was 24% and for the controls group was 18%. In addition, the 1G/2G genotype frequency was 68% for the patients group, and for the controls group was 82% (P=0.83). The results of Tetra-arms PCR genotyping of the samples were confirmed by sequencing.
Conclusion: The findings indicated that in Iranian patients, MMP-1 -1607 1G/2G (rs1799750) was not significantly associated with periodontal disease. It is recommended to take more samples from different parts of Iran to confirm the results.
Pedram Abedi, Maysam Mirzaee, Sahba Amini, Mohammad Mehdi Naghibi Sistani,
Volume 33, Issue 4 (1-2021)
Abstract
Background and Aims: Information collection about the malocclusion and oral health in blind children is essential to plan for their education in schools. Therefore, this study aimed to evaluate the status of malocclusion and oral health among blind children and teenagers in Mazandaran, Iran.
Materials and Methods: The population of this included 150 blind children and teenagers aged from 10 to 18 years who were referred to the public and private centers cities including Sari, Babol, Freydoonkenar, and Babolsar in Mazandaran, Iran in 2019. Information on maxillary occlusion status, oral hygiene index, and DMFT index was extracted through clinical examination. Data were analyzed using SPSS23 software by ANOVA and Chi-square tests in addition to the descriptive statistics.
Results: The mean DMFT was 10.25 (SD 4.67). Oral hygiene status was good in 16% of participants, moderate in 82%, and poor in 2%. There was no significant difference between girls and boys in terms of oral hygiene status (P=0.5). In all cases, 35.8% had class I malocclusion, 29.8% had class II malocclusion, and 14.6% had class III malocclusion. Class II malocclusion was more common in girls and class I in boys (P=0.01).
Conclusion: Dental caries and occlusal abnormalities were common among blind children and adolescents living in Mazandaran province. Addressing the oral and dental needs of the blind should be a priority for health policymakers in this province in Iran.
Elahm Siasi Torbati, Nafiseh Tavakkoli, Kumars Amini,
Volume 33, Issue 4 (1-2021)
Abstract
Background and Aims: Periodontitis is an inflammatory multifactorial disease in oral tissues and many genetic reasons and environmental factors responsible. Vitamin D deficiency has been determined to be related to periodontal disease. This aim of this study was to investigate the association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis in 100 patients (as patient and control groups).
Materials and Methods: Blood samples from 50 patients and 50 control groups were selected and DNA from the samples was extracted by the DNA extraction kit. Genotyping was used by Tetra Arms-PCR method. The use of sequencing was confirmed by the Tetra Arms-PCR genotyping results. Then, statistical analysis was performed using SPSS statistics 20 software and T-test.
Results: Frequency of AA, AC, and CC Genotypes were 25 (50%), 14 (28%), and 11 (22%) in patients’ cases, and 26 (52%), 16 (32%) and 8 (16%) in controls, respectively. AA genotype was the highest genotype between the patient and control groups. Statistical analysis showed no significant association between this type of polymorphism and periodontitis disease in the studied samples (P=0.67).
Conclusion: This finding showed there was not significant association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis disease in the studied samples. To confirm the results of this study, further studies with large sample size and different types of population are recommended.
Fatemeh Hemmati, Mansour Bayat, Kumarss Amini,
Volume 34, Issue 0 (5-2021)
Abstract
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method.
Materials and Methods: For this study, 100 individuals including 50 patients and 50 healthy individuals were recruited and examined by a periodontist. After identifying sulfate-reducing bacteria by biochemical tests and specific media, the effect of aloe vera extract on them was investigated and the expression of BFR gene against housekeeping gene (16srDNA) was determined by Real time- PCR test via T-test analysis method.
Results: The data showed that 12 strains of sulfate-reducing bacteria were isolated from the samples, 5 of which had BFR gene and their gene expression was significantly reduced by aloe vera gel (P<0.05). |
Conclusion: The data of this study in proving the anti-biofilm and antibacterial effects of aloe vera extract showed that the expression of the target gene is reduced. It seems that this substance can be used as an alternative to oral hygiene chemicals.
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method.
Materials and Methods: For this study, 100 individuals including 50 patients and 50 healthy individuals were recruited and examined by a periodontist. After identifying sulfate-reducing bacteria by biochemical tests and specific media, the effect of aloe vera extract on them was investigated and the expression of BFR gene against housekeeping gene (16srDNA) was determined by Real time- PCR test via T-test analysis method.
Results: The data showed that 12 strains of sulfate-reducing bacteria were isolated from the samples, 5 of which had BFR gene and their gene expression was significantly reduced by aloe vera gel (P<0.05). |
Conclusion: The data of this study in proving the anti-biofilm and antibacterial effects of aloe vera extract showed that the expression of the target gene is reduced. It seems that this substance can be used as an alternative to oral hygiene chemicals.
Noushin Khandan Dezfuli, Majid Sadeghpour, Mojgan Sarabi Nobakht, Elham Estabarghi, Kumarss Amini,
Volume 34, Issue 0 (5-2021)
Abstract
Background and Aims: Periodontitis is a common and inflammatory infectious disease that causes damage to the tissues supporting the tooth and consequent tooth loss. Periodontal disease is a multimicrobial and multifactorial disease and important anaerobic bacteria are involved in periodontal infection. TGF-1β is one of the growth factors and anti-inflammatory cytokines that play a crucial role in the repair of periodontal lesions. The aim of this study was to evaluate Tetra Arms-PCR with high sensitivity and specificity, which can be used to evaluate the genomic polymorphism among oral samples and show the relationship between TGF-1β and periodontal disease.
Materials and Methods: The present study was a case-control study in the periodontology department of Kerman Dental School. Sampling was done from 100 samples including 50 healthy individuals and 50 patients with microbial periodontal infection. Genotype was analyzed using DNA extracted from the blood of patients by PCR -ARMs-Tetra to determine the relationship between TGF-1β genomic polymorphism and periodontitis. Statistical analysis was performed with SPSS19 software and one-way ANOVA.
Results: The samples were culture positive, therefore, more than 65% of the isolated bacteria were anaerobic which included: Prevotella, Porphyromonas, Fusobacterium, Peptostreptococcus anaerobic. The results of Tetra PCR ARMs after sequence frequency were genotype CC allele (25%), CT allele (20%), TT allele (5%). Percentage of control group were CC allele (20%), CT allele (24%), and TT allele (6%). The frequency of C and T alleles in the patient group was 70% and 30%, and in the control group 63% and 37%, respectively with no significant difference between two groups (P=0.83). |
Conclusion: According to the results of this study and the application of anaerobic conditions, forced anaerobic bacteria can be isolated from clinical specimens of oral infections and by Tetra Arms-PCR no significant relationship between TGF-1β genomic polymorphism and periodontitis was observed. In addition, therer was no significant difference in the frequency of alleles and genotypes between the control and patient groups.
