Journal of Dental Medicine

Statement Problem: A few studies have been conducted about bioglass posts.
Aim: The aim of this study was to compare bioglass posts with prefabricated metallic posts in clinical performance of extensive composite restorations for anterior endodontically treated teeth.

Materials and Methods: Sixty endodontocally maxillary anterior teeth, with horizontally or vertically destruction, were selected. Teeth were divided into two groups based on the kind of post: Metallic prefabricated parapost and bioglass post. Each group was divided into three subgroups based on anterior bite: normal, deep bite and edge to edge. Gutta-percha was removed from 2/3 of canal length for parapost and 1/3 for bioglass post. After etching with phosphoric-acid (37%) and applying dentine bonding syntac, Duo cement was used for the adhesion of bioglass post and a self cured composite (Degufil) for parapost. Restoration was done with a hybrid composite (Heliomolar). Follow up studies, radio-graphically and clinically, were done every three months for a 1.5-year period. Exact Fisher and Pearson tests were used for data analysis.
Results: Apical lesion was not observed in any of the radiographs. Post seal was increased by resin cement and dentin bonding agent. Post type did not significantly affect on the clinical success rate of the restorations. The retention of restoration, for both posts, was the same. Crown destruction had no significant effect on success rate. The type of anterior bite had a significant effect on success rate, as the total 6.6% failure rate was related to the patients with anterior deep bite.
Conclusion: It is suggested to use metallic paraposts and bioglass posts, in extensive composite restorations for patients with deep-bite, more conservatively.

Statement of Problem: Total removal of tissues and remnant microorganisms as well as canal shaping are the essential objectives of endodontic therapy. A successful endodontic treatment is obtained through Shilder’s principals, however complete observation of this technique using stainless steel files manually is problematic and time-consuming. Modern technology, in order to eliminate such problems, has presented new facilities such as Nickel-Titanium (NiTi) files and engine driven instruments.

Purpose: The aim of this in-vitro study was to compare the canal debridement efficiency of three engine driven instruments: Rotary, Reciprocal and Vertical.

Materials and Methods: In this experimental study, 60 mesial roots of human first and second mandibular molars were divided into three groups randomly. In each sample, one canal was considered as case, the other one as control. Files used in Reciprocal and vertical groups were of handy Ni-Ti type and in rotary group, rotary Ni-Ti files were used. After debridement, the roots were sectioned at 3mm and 5mm from anatomic apex, stained and examined under light microscope. Comparison criteria between case and control groups were based on residual debris and predentin and the level of root canal preparation and shaping after debridement. Data were subjected to kruskal-Wallis non-parametric test.

Results: There was no significant difference between the efficiency of debridement at 3mm and 5mm sections between all groups. But difference in time consumption was significant ranked from the shortest to the longest as rotary, reciprocal and vertical.

Conclusion: The efficiency of debridement between the three automated instruments was approximately equal, however the instrumentation time was different between three groups. Rotary system was the fastest one, as compared with reciprocal (second) and vertical (last). It may be concluded that rotary system has a superiority over the other two groups in conventional root canal therapies.

Background and Aims: The purpose of this study was to design a new device assisting dental students to measure the tapering of the prepared teeth for fixed crown prosthesis in which the desired tapering for better clinical prognosis could be obtained.

Materials and Methods: The designed device in this study had two arms that were placed on two opposite walls of the prepared teeth. To evaluate the validity and reliability of this device, 3 dies with different degrees (13, 29 and 45°) were used and 20 teeth were prepared by dental students with undefined tapering degrees. The measurements of tapering were performed by six last year-dental students in two different time periods. Comparison of errors in measurements on standard dies between observers and between time intervals were analyzed by (Wilcoxon) Paired t-test with a significance level of α=0.05.

Results: For the three dies used in this study, we compared the differences for each measurement taken by observers with the standard taper of the dies. Maximum difference of measurements with true standard die tapering was 1 degree. A number of 6 measurements were under the true standard tapers and 2 measurements were above it. Interclass Correlation Coefficients (ICC) of the first and second measurements on prepared teeth were 97.4 and 97.5, respectively.  There were no significant differences between different observers or two measurements of the same observer in different time intervals (P>0.05).

Conclusion: The results indicated that this device can be valid and reliable for measuring the angle of convergence of the prepared teeth and can be used as an educational device in dental schools as a replacement for previous tapering measurement methods.

Background and Aims: Periodontitis is one of the most common oral diseases with the various incidence rates in different populations. A number of bacteria are considered as the major etiologic agents of periodontitis. The aim of the present study was to determine the prevalence of periodontopathogen bacteria in patients using both PCR and culture techniques.
Materials and Methods: In this study, one-hundred patients (including 62 females and 38 males with an average age of 49±11.5 years) with adult periodontitis referred to periodontics department of School of Dentistry/Tehran University of Medical Sciences were investigated. The samples were taken and sent immediately to the laboratory for culture and molecular evaluation. The PCR was performed using specific primers and the statistical analysis of data was performed using SPSS statistic software and McNemar test.
Results: The results demonstrated that the total detection rate in culture method was 64%. The rate of Aggregatibacter actinomycetemcomitans (Aa) was 28% which was significantly higher than that of Porphyromonas gingivalis (Pg) (6%) and Prevotella intermedia (Pi) (3%). 27% of cases showed mixed bacterial growth. 65% of patients were positive using molecular method. The rate of Aa (30%) was significantly higher than that of Pg (7%) and Pi (5%). The mixed PCR positive rate containing of Aa, Pg and Pi was (23%).
Conclusion: In this study, it was found that most of the bacteria isolated using culture and molecular methods were Aa, Pg and Pi, respectively. Although the detection frequencies of both techniques were similar, the specificity, sensitivity and bacterial detection speed of the PCR technique is obviously higher. Therefore, the use of molecular techniques is strongly recommended. However, both techniques seem to be suitable for microbiological diagnostics.

Background and Aims: Skeletal class III malocclusions are considered as one of the most complex and difficult orthodontic problems to diagnose and treat. Facial appearance and occlusion problems make the patients to seek treatment as soon as possible. The purpose of this study was to evaluate the outcomes of early treatment with facemask compared to the patients who had no treatment.

Materials and Methods: For this retrospective study, 38 growing patients (mean age, 8.5 years old) with class III dentoskeletal malocclusion were divided into 2 groups. 23 patients were treated with facemask and 15 patients who did not receive any treatment were assigned as control group. Lateral cephalograms were taken before and after treatment and dentoskeletal changes were estimated. Data were analyzed using Wilcoxon signed ranks test.

Results: The results revealed statistically significant forward displacement of maxillary bone and upper incisors (P<0.001), and downward-backward rotation of mandible in facemask group (P<0.001). Increased lower facial height was seen in both treatment and untreated groups. However, in untreated group some measurements revealed forward displacement of maxillary complex as well.

Conclusion: It seems that in class III patients treating with facemask is a better choice than ChinCap and decision making for early treatment in class III patients should be considered seriously. Moreover, specific attention to the type of patient’s skeletal discrepancy is necessary. In class III patients, facemask might be a better option than other appliances.

  Background and Aims: In the last decade, several studies have reported the isolation of stem cell population from different dental sources, while their mesenchymal nature is still controversial. The aim of this study was to introduce the isolating methods for stem cells from human dental pulp and to determine their mesenchymal nature before differentiation.

  Material and methods: One of the best sources for stem cell is dental pulp tissue. Dental Pulp Stem Cells (DPSCs) would be the most convenient source of stem cells because teeth were easy to retrieve and removed throughout life. Pulp is a specialized connective tissue including blood and lymph vessels, nerves, and the interstitial fluid. DPSCs can be found within the ‘‘cell rich zone’’ of pulp. DPSCs have been isolated for the first time in 2000 by Gronthos these cells exhibited a differentiation potential for odontoblastic, adipogenic and neural cytotypes. Gronthos isolated stem cells in 2 different methods: The enzymatic digestion method and the second was out growth, these cells could be cryopreserved in liquid nitrogen. It has also been shown that human DPSCs can be used for complex structures such as pulp or woven bone formation in vivo.

  Conclusion: DPSCs originate from the cranial neural crest and have neural characteristics such as the expression of neurotrophins. Therefore, DPSCs may represent a promising source in cell therapy for neurological disorders. Characterization of these cells and determination of their potentialities in terms of specificity of regenerative response will form the foundation for development of new clinical treatment modalities, whether involving directed recruitment of the cells and seeding of stem cells at sites of injury for regeneration or use of the stem cells with appropriate scaffolds for tissue engineering solutions. Such approaches will provide an innovative and novel biologically based on new generation of clinical treatments for dental disease.