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Showing 5 results for Masoum
Immunohistochemical evaluation of p21 and cyclin D1 expression in ameloblastoma of the jaws
M. Khalili , M. Eslami , P. Masoumi ,
Volume 20, Issue 1 (4 2007)
Abstract

Background and Aim: The cell cycle is an important event in tumor growth and differentiation and several molecules are involved in this process. The aim of this study was to evaluate the expression of cyclin D1 (a cell cycle inducer) and p21 (a cell cycle inhibitor) in ameloblastoma of the jaws.

Materials and Methods: In this cross-sectional study, 40 cases of ameloblastoma were selected from the archive of oral pathology department. 3 micron sections were cut from paraffin blocks and immunohistochemically stained with antibody against cyclin D1 and p21waf. Stained cells were counted using an eyepiece graticule and labeling index was calculated. Data were analyzed by SPSS version 11.5 for windows using Mann-Whitney and Wilcoxon signed ranked tests with p<0.05 as the level of significance.

Results: Expression of cyclin D1 protein was detected in nuclei of many tumoral cells. The expression of cyclin D1 in solid and unicystic ameloblastoma and also between its follicular and plexiform variants was not statistically different (P>0.05). There was no statistically significant difference in expression of cyclin D1 between peripheral and central cells (P>0.05). Expression of p21 protein was detected in nuclei of some tumoral cells. There were no statistically significant differences between p21 expression in unicystic and solid ameloblastoma (P>0.05). P21 expression was statistically different between plexiform and follicular variants of ameloblastoma (P=0.049). The difference between p21 expression in peripheral cells of plexiform and follicular variants was statistically significant (P=0.009). This was not observed in central cells. There was no statistically significant relation between p21 and cyclin D1 expression in ameloblastoma (P>0.05).

Conclusion: Based on the results of this study, cyclin D1 expression in ameloblastoma is in high level and it could have an important role in the process of tumorigenesis. P21 expression in ameloblastoma is very faint and its possible effects need further investigation.


Effect of chitosan on osteogenic properties of mesenchymal stem cell of exfoliated deciduous teeth
Tahereh Masoum, Iraj Amiri, Rezvan Rafatjou,
Volume 26, Issue 1 (3-2013)
Abstract

Background and Aims: The exfoliated human deciduous tooth contains multipotent stem cells [Stem Cell from Human Exfoliated Deciduous tooth (SHED)] that identified to be a population of highly proliferative and clonogenic. These cells are capable of differentiating into a variety of cell types including osteoblast/osteocyte, adiopcyte, chondrocyte and neural cell. The aim of this study was to evaluate the differentiation of SHED to osteoblast in standard osteogenic medium and comparing the results with medium which supplemented with glucosamine in form of chitosan.

Materials and Methods: Dental pulp cells were isolated from freshly extracted primary teeth, digested with 4 mg/ml collogenase/dispase, and grown in Dulbecco's modified Eagle's medium with 10 percent fetal bovine serum. The clonogenic potential of cells was performed after 3 weeks of culture. Flowcytometric analysis, performed at day 21 of culture to identify surface markers of mesenchymal stem cells. The cells from 3rd passage were used for osteogenic differentiation in routine osteoinductive medium. Chitosan (10 μg/ml) was added to the culture medium of case group. Alizarin Red Staining and Alkaline Phosphatase (ALP) activity were done to evaluate osteogenic differentiation in the developing adherent layer on the third passage. The results were analyzed using T-test. For the analysis of normal distribution of data, non-parametric Kolmogrov-Smirnov test was used.

Results: The colonogenic efficiency was more than 80%. Flowcytometric analysis showed that the expression of mesenchymal stem cell marker CD90, CD105 and CD146 were positive in SHED, while hematopoietic cell marker CD34, CD45 and endothelial cell marker CD31 were negative. Quantitative analysis of Alizarin Red Staining demonstrated that: mineralized nodule formation was higher in the group supplemented with glucosamine (chitosan). Results from Alkaline Phosphatase activity test, on day 21, demonstrated a significantly higher ALP activity in the group supplemented chitosan (P<0.001).

Conclusion: Stem cells isolated and cultured from exfoliated deciduous teeth pulp can be differentiated to osteoblast. Addition of chitosan can be beneficial to promote osteogenic differentiation of these cells.


Comparison of setting time of an Iranian MTA with a foreign MTA
Sakineh Arami, Sakineh Arami, Masoumeh Hasani Tabatabaei, Seyed Reza Najafzadeh, Ahmadreza Shamshiri, Zohreh Moradi,
Volume 30, Issue 4 (1-2018)
Abstract
Background and Aims: According to many uses of MTA in different treatments, studying about its properties like the setting time, is of great importance. The aim of this study was to compare the initial and final setting time of an Iranian MTA and a foreign MTA.
Materials and Methods: Seven samples were in each group; for measurement of initial and final setting time of MTAs, a mold with dimension of 2×10 mm was prepared. The cements after mixation were put into an incubator with temperature of 37ₒC and humidity of 90%. After mixing of cements, a Gilmore needle with a weight of 100 gr and active tip of 2 mm was used vertically on the surface of MTA for measurement of the initial setting time. Setting was measured by the start of cement mixation to the time the tip of the needle didn’t penetrate to the surface of MTA. For measurement of the final setting time a Gilmore needle with a weight of 456 gr and active tip of 1mm was used vertically on the surface of MTA. The ANOVA test followed by Tukey test was used for comparison of the mean setting time between an Iranian and foreign MTA. The statistical difference was considered significant at the level of 0.05.
Resultes: The mean initial setting time Angelus and Root MTAs were 13.57±0.91 and 11.14±0.92 min, the mean final setting of Angelus and Root MTA, were 48.93±1.54 and 37.86±1.5 min while the initial setting time of Angelus MTA lasted for 0.53±43.2 more min than Root MTA (P=0.001). The final setting time of Angelus MTA took longer for 110.7±0.82 min than Root MTA (P=0.001).
Conclusion: The initial and final setting time of the Iranian MTA was shorter than the foreign one and thus it could a more alternative option in this respect.

Effects of bleaching on color stability of enamel lesions treated with resin infiltrant
Mansoureh Emami Arjomand, Seyde Sahar Masoumi, Haleh Heshmat, Zahra Nouralizadeh,
Volume 35, Issue 0 (5-2022)
Abstract
Background and Aims: Considering the preventive and therapeutic effects of resin infiltration (RI) and the unknown effect of bleaching on the enamel treated with this method, this in-vitro study aimed to assess the effect of bleaching on color stability of enamel lesions treated with RI.
Materials and Methods: In this in-vitro, experimental study, enamel-dentin samples measuring 5×5×3 mm were prepared on the buccal and lingual surfaces of 30 extracted humans third molars with no caries, cracks or enamel defects by a microtome. The specimens were divided into three groups of 10 specimens: 1) sound enamel (control), 2) demineralized enamel, 3) demineralized enamel plus RI. White spot lesions were artificially created by immersing the samples in hydroxyethyl cellulose demineralizing gel with a pH of 4.5 for 4 days. Next, Icon RI was applied on the samples in group 3. The baseline color of the specimens was measured using a spectrophotometer. Then, 35% hydrogen peroxide was applied on the specimens in three cycles and time interval of 10 min. After the end of the last cycle, the samples were completely washed for 1 min and then, underwent colorimetry again. Data were analyzed using one-way ANOVA.
Results: The results of this study showed that the highest level of color change (ΔE) in the demineralized group was 6.3±2.003, while the least amount of ΔE in the enamel lesion group treated with resin infiltration was 3.54±0.98 and in the control group ΔE was 4.13±1.14. Therefore, there was a significant difference between demineralized and treated by infiltratant groups (P=0.005). However, there was no significant difference between control group and treated with resin infiltration group (P=0.54).
Conclusion: Bleaching induced a less change in the color of the enamel lesions treated by resin infiltration compared with that of the demineralized teeth.
 
 

Evaluation of post-extraction complications of mandibular impacted third molar using piezo-surgery versus conventional rotary method
Ehsan Aliabadi, Hamidreza Masoumi,
Volume 36, Issue 0 (5-2023)
Abstract
Background and Aims: The surgical removal of mandibular third molar is frequently accompanied by significant postsurgical complications, and different protocols have been described to decrease such complications. The aim of this study was to compare the postoperative complications of mandibular impacted third molar removal with two conventional rotary and piezoelectric methods.
Materials and Methods: In this study 27 patients with bilateral mandibular impacted third molars were selected. The patients’ teeth were extracted by the piezoelectric method on a random side and the opposite tooth was extracted by conventional rotary technique. On operation and post operation days, edema, pain, and mouth opening were assessed. The duration of each operation was also measured. Data were analyzed by Mann-Whitney test and independent t-test.
Results: The mean duration of operation in the rotary group was less than that of the piezoelectric group, which was statistically significant (P=0.001). Pain was significantly higher in the rotary group on days 0 to 5 (P<0.05). Whereas, difference in pain on day 6 was not statistically significant (P=0.067). There was no significant difference between the two groups in each day after surgery regarding mouth opening (P>0.05). Edema was significantly higher in the rotary group on the first, third, and fifth days after surgery (P<0.05). Also, edema was higher in the rotary group on the seventh day after surgery, but not statistically significant (P=0.076).
Conclusion: According to the results of the present study, it can be concluded that pain and swelling in patients after impacted tooth surgery using time consuming piezoelectric technique can be reduced.

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