Journal of Dental Medicine

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In this study, by cluster simple random sampling, the prevalence of ectopic of first permanent molar in 8 years old Tehran school children is considered and determined as 0.87 percent The involvement of maxilla was more than mandible and the difference was significant. Bilateral involvement was more than unilateral and boys were more- than girls, how ever the differences were not significant. As for as the geographical distribution is considered, the differences were not noticeable, either.

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Statement of Problem: Pulpotomoy is considered as one of the most common treatments among primary teeth. Therefore, search for an appropriate and safe material seems necessary. Studies have proved ferosulfate as a therapeutic material in pulpotomy.

Purpose: The goal of the present study was to compare clinical and radiographic changes of pulpotomy using ferosulfate and formocresol in primary molars among 3-8 year old children.

Materials and Methods: In this clinical trial study, fifteen 3-8 years old children, referred to the pedodontics department, faculty of Dentistry, Tehran University of Medical Sciences were selected. All subjects did not show any systemic disease, with at least two clinically and radiographically accepted posterior teeth for pulpotomy technique. Totally, forty- three teeth were studied. For each patient both materials were used. Following treatment, patients were investigated, clinically and radiographically in 3, 6, 9 and 12 month follow-ups. During follow-up periods, teeth with failures were not excluded and investigated. For data analysis, Chi-square and Fisher’s-exact tests were used and treatment success was evaluated, based on clinical as well as radiographic findings.

Results: In all follow-up periods (3, 6, 9 and 12 months after treatment), pulpotomies with formocresol showed more success rate (73.7%) comparing to those with ferosulfate (70.8%), although the difference was not statistically significant (P>0.05). Statistical tests revealed no significant difference between two different treatment techniques regarding pain, external and internal resorption, calcified metamorphosis, abcess, apical root resorption, apical and interradicular radiolucency.

Conclusion: Although ferosulfate, due to its less toxicity, may be considered as a proper substitute for formocresol in primary molars pulpotomies, more studies with longer-term follow up and larger sample size are required to determine its long-term effects.

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Background and Aim: Elimination of microorganisms and their byproducts from root canal system is one of important aims of root canal therapy. This object is gained by using of many chemomechanical techniques but with noncertain success. A new method is used of nonpathogenic bacteria for growth inhibition of pathogenic bacteria, Antibiosis, in root canal therapy.The aim of this study was in vitro evaluation of antimicrobial effect of probiotics, such as Lactic Acid Bacteria (LAB) on the infected root canal bacteria.

Materials and Methods: Isolated bacteria from infected root canal were grown and then scattered onto the muller Hinton agar plates which contain wells, LAB, extracted from dairy products, were added into these wells, Inhibition effected of LAB was determined. Furthermore the sample taken from the inhibition zone and possible resistant monoclonal bacteria also were identified, then 6 sensitive and 14 resistant samples were selected and E. faecalis species were added to them Then antimicrobial effects of LAB on these samples was reevaluated.

Results: The results showed that 66.7% of the samples were sensitive at least to one type of LAB, and 33% were resistant to all kind of LAB. Meanwhile the outgrowing anaerobic bacteria inside the inhibition zone were from the low frequency oral bacterial flora. Furthermore, adding E. faecalis to the samples caused more sensitivity of them to LAB. Mc-Neamar test recognized the difference significant.

Conclusion: This study showed that the LAB inhibit growth of the pathogenic root canal bacteriae. Furthermore, presence of E. faecalis reinforces the antimicrobial effect of LAB. It seemed that LAB maybe have potential to use in endodontic practice for elimination of root canal infections.

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Background and Aims: Moisture contamination of etched enamel during application of sealant is the most frequently cited reason for sealant failure. In previous studies, some hydrophilic bonding agents have been shown to bond to etched enamel after salivary contamination. The purpose of this study was to evaluate the enamelsealant interface following the use of primer on the saliva contaminated and/or moist etched enamel.

Materials and Methods: 40 extracted human incisors were selected. The enamel surfaces were etched for 20 seconds with an acid gel and rinsed for 30 seconds and dried until chalky surface was observed. Teeth were randomly divided into four treatment groups (n=10). Sealant (Deguseal mineral, Degussa) was applied on (1) etched enamel surface as a standard control group, (2) in conjunction with using primer material on moist etched enamel, (3) in conjunction with using primer material on salivary contaminated etched enamel, and (4) on wet contaminated etched enamel surface as a negative control group. Then, the specimens were observed with scanning electron microscopy (SEM) at ×1400 magnification. To evaluate the enamel resin interface, the number of resin tags was counted. Data were analyzed using one-way ANOVA.

Results: The results showed that primer used on moist enamel under sealant had no significantly difference in number and shape of tags in comparison with standard group (P>0.05). Application of primer on saliva contaminated enamel surface under sealant significantly reduced the number of tags and changed the shape and pattern of tags (P<0.001). Following application of sealant on contaminated and etched enamel alone, no resin tags formed.

Conclusion: Application of primer relatively reduced sensitivity to saliva contamination but the pattern was changed and number of tags reduced. Therefore, primer application might be useful whenever the isolation is not possible. However, it should be emphasized that restrict isolation in sealant therapy is recommended.

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  Background and Aims: Considering the complications of root canal system, debridement would not be effective only by mechanical instrumentation. Therefore, root canal irrigants with the antimicrobial effect that can eliminate more microorganisms during treatment, becomes important. The aim of this study was to compare the antimicrobial effects of 2% thyme essence, 2.5% sodium hypochlorite (NaClO), and normal saline as root canal irrigants for primary molar teeth.

  Materials and Methods: This study was performed on 34 primary molars. Subjects were randomly divided into three groups (N=10). The root canals of the teeth were prepared up to the file #35 and all of the specimens were sterilized, and were then contaminated with entrococccus faecalis suspension (Enterococcus Faecalis ATTC 29212). After 48 hours of incubation, the specimens of each group were irrigated with corresponding irrigants. Then, the microbial specimens were collected with sterile paper points. After incubation, the numbers of Colony Forming Units (CFU) were counted using colony counter. Data were analyzed using Smirnov-Kolmogorov, Kruskal-Wallis and Mann-Whitney tests.

  Results: Sodium hypochlorite showed the highest antimicrobial effect and showed significant differences compared with normal saline (P<0.001). However, the differences were not significant with thyme essence 2% (P=0.3).

  Conclusion: Thyme essence could be considered as a natural irrigator with a comparable antiseptic effect to sodium hypochlorite.