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The effect of nano-hydroxyappatite solution on the permanent tooth remineralization following exposure to soft beer (in situ)
Haghgoo Roza , Haghgou Hamid Reza , Abbasi Farid , Tavakkoli Mohammad ,
Volume 27, Issue 4 (1-2015)
Abstract

  Background and Aims: The main cause of erosion is acid exposure . Side effects of erosion necessitate therapeutic agents’ uses. The aim of this study was to investigate the effects of nano- hydroxy apatite in tooth remineralization following exposure to soft beer.

  Materials and Methods: This in vitro experimental study was conducted on 18 human impacted third molars that had been surgically extracted. The microhardness of specimens was measured. Then teeth were exposed to soft beer and their secondary microhardness was measured. The teeth were divided into 2 groups (water and nano-hydroxy apatite solution) and were placed on 9 orthodontics appliances and delivered to 9 volunteers. These volunteers placed the tooth on one side in water for 5 minutes and the tooth in opposite side in nano-hydroxyapatite solution. This application was repeated 6 times a day for 10 days. The microhardness of teeth was measured again. Data were analyzed using Paired T-test.

  Results: The tooth enamel microhardness reduced after exposure to soft beer significantly (P=0.04). The microhardness of 9 teeth after being in water showed significant changes (P=0.012). The microhardness of 9 teeth significantly changed after exposure to nano -hydroxyapatite solution (P=0.001) .

  Conclusion: Based on the results of this study, 10% solution of nano- hydroxy appatite could restore the erosive lesions .


Study of association between rs7975232 polymorphism in vitamin D receptor gene and periodontitis by Tetra Arms PCR
Elahm Siasi Torbati, Nafiseh Tavakkoli, Kumars Amini,
Volume 33, Issue 4 (1-2021)
Abstract
Background and Aims: Periodontitis is an inflammatory multifactorial disease in oral tissues and many genetic reasons and environmental factors responsible. Vitamin D deficiency has been determined to be related to periodontal disease. This aim of this study was to investigate the association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis in 100 patients (as patient and control groups).
Materials and Methods: Blood samples from 50 patients and 50 control groups were selected and DNA from the samples was extracted by the DNA extraction kit. Genotyping was used by Tetra Arms-PCR method. The use of sequencing was confirmed by the Tetra Arms-PCR genotyping results. Then, statistical analysis was performed using SPSS statistics 20 software and T-test.
Results: Frequency of AA, AC, and CC Genotypes were 25 (50%), 14 (28%), and 11 (22%) in patients’ cases, and 26 (52%), 16 (32%) and 8 (16%) in controls, respectively. AA genotype was the highest genotype between the patient and control groups. Statistical analysis showed no significant association between this type of polymorphism and periodontitis disease in the studied samples (P=0.67).
Conclusion: This finding showed there was not significant association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis disease in the studied samples. To confirm the results of this study, further studies with large sample size and different types of population are recommended.

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