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Isolation and characterization of mesenchymal stem cells derived from dental pulp and follicle tissue of human third molar tooth
F. Mashadi Abbas, S. Mojarrad, Z. Yadegary, B. Sharifi,
Volume 24, Issue 2 (23 2011)
Abstract

Background and Aims: In the last decade, several studies have reported the isolation of stem cell population from different dental sources, while their mesenchymal nature is still controversial. The aim of this study was to isolate stem cells from mature human dental pulp and follicle and to determine their mesenchymal nature before differentiation based on the ISCT (International Society for Cellular Therapy) criteria.
Materials and Methods: In this experimental study, intact human third molars extracted due to prophylactic or orthodontic reasons were collected from patients aged 18-25. After tooth extraction, dental pulp and follicle were stored at 4°C in RPMI 1640 medium containing antibiotics. Dental pulp and follicle were prepared in a sterile condition and digested using an enzyme solution containing 4mg/ml collagenase I and dispase (ratio: 1:1). The cells were then cultivated in α-MEM medium. Passage-3 cells were analyzed by flow cytometry for the expression of CD34, CD45, CD 73, CD90 and CD105 surface markers.
Results: Dental pulp and follicle were observed to grow in colony forming units, mainly composed of a fibroblast-like cell population. Flow cytometry results showed that dental pulp and follicle are highly positive for CD73, CD90 and CD105 (mesenchymal stem cell markers) and are negative for hematopoietic markers such as CD34 and CD 45.
Conclusion: In this study we were able to successfully confirm that dental pulp and follicle stem cells isolated from permanent third molars have a mesenchymal nature before differentiation. Therefore, these two sources can be considered as an easy accessible source of mesenchymal stem cells for stem cell research and tissue engineering.


Reasonable application of antibiotic prophylaxis in maxillofacial trauma: Literature review
Afshin Yadegari Naeeni, Masoud Vatani, Bahareh Botlani Yadegar,
Volume 29, Issue 1 (7-2016)
Abstract

Background and Aims: Despite advances in trauma management, treatment of the consequent infections has remained a major challenge. Antibiotic prophylaxis has been widely applied to reduce such infections. Although bacteria are present in most body parts, severe infections after treatment are less frequent in the head and neck of healthy individuals. The aim of the present study was to review the reasonable application of antibiotic prophylaxis in maxillofacial trauma.

Materials and Methods: In this review article, PubMed and Google Scholar databases were searched for studies on antibiotic prophylaxis in maxillofacial trauma published during 2000-2014.

Conclusion: Antibiotics were not prescribed for tears and small clean wounds in the face and mouth. However, prophylaxis was applied for extensive mouth injuries which involved the facial skin. In case of maxillofacial fractures, 24-hour administration of antibiotics sufficed for compound fractures of the mandible and other parts of the face. Antibiotics were not required in other types of fractures. Prophylaxis should be applied over short pre- or post-operative periods based on the severity and complexity of maxillofacial fractures and their relations with intra- and extraoral environments. Apparently, more detailed studies are warranted to further clarify the subject.


The effect of adding fluorinated graphene nanoparticles on fluoride release in a commercial fissure sealant: an in vitro study
Rezvan Arian, Namazi, Hanieh Nojedehian, Farhood Najafi, Zahra Yadegari, Roxana Karbaschi, Maryam Torshabi, Sarvin Soleimanpoor,
Volume 37, Issue 0 (3-2024)
Abstract
Background and Aims: Using fissure sealant is one of the most effective methods of preventing pit and fissure decay. Fluoride has been added to various materials as a known anti-caries agent. Fluorinated graphene (FG) has gained attention due to its unique properties. The specific structural characteristics of graphene fillers, besides having antibacterial properties by increasing the rate of fluoride release and charge and neutralizing the acidic pH of the environment, is a suitable option in many treatments. This study investigated the effect of addition of fluorinated graphene nanoparticles on the fluoride release in Fissurit®, a commercial fissure sealant.
Materials and Methods: In this in vitro study, fluorinated graphene oxide (FGO) with bright white color was prepared. After synthesis, 0, 1, 2 and 4 by weight percent were added to the fissure sealant (Fissurit®). Then, the release of fluoride in this material was measured and compared with the commercial fissure sealant containing fluoride (Fissurit®).
Results: According to the obtained results, the amount of fluoride released from groups with different percentages of FGO had a direct relationship with the percentage of FGO addition. Also, at different times, there was a significant difference between the groups with FGO and the control group without FGO (P<0.001).
Conclusion: Addition of FGO to the fluorinated fissure sealant caused the release of fluoride and the possibility of recharging it. The power of releasing fluoride and its recharging in fissure sealant with FGO was higher than the fluorinated fissure sealant, but its fluoride was discharged at a faster rate.

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