Journal of Dental Medicine

Background and Aims: In the last decade, several studies have reported the isolation of stem cell population from different dental sources, while their mesenchymal nature is still controversial. The aim of this study was to isolate stem cells from mature human dental pulp and follicle and to determine their mesenchymal nature before differentiation based on the ISCT (International Society for Cellular Therapy) criteria.
Materials and Methods: In this experimental study, intact human third molars extracted due to prophylactic or orthodontic reasons were collected from patients aged 18-25. After tooth extraction, dental pulp and follicle were stored at 4°C in RPMI 1640 medium containing antibiotics. Dental pulp and follicle were prepared in a sterile condition and digested using an enzyme solution containing 4mg/ml collagenase I and dispase (ratio: 1:1). The cells were then cultivated in α-MEM medium. Passage-3 cells were analyzed by flow cytometry for the expression of CD34, CD45, CD 73, CD90 and CD105 surface markers.
Results: Dental pulp and follicle were observed to grow in colony forming units, mainly composed of a fibroblast-like cell population. Flow cytometry results showed that dental pulp and follicle are highly positive for CD73, CD90 and CD105 (mesenchymal stem cell markers) and are negative for hematopoietic markers such as CD34 and CD 45.
Conclusion: In this study we were able to successfully confirm that dental pulp and follicle stem cells isolated from permanent third molars have a mesenchymal nature before differentiation. Therefore, these two sources can be considered as an easy accessible source of mesenchymal stem cells for stem cell research and tissue engineering.

Background and Aims: Nowadays, calcium hydroxide is used as a dressing for canal sterilization and repair progression of apical lesions. The aim of this study was to investigate the effect of calcium hydroxide as an intracanal medicament on the apical microleakage of root filling.
Materials and Methods: In this experimental study, 46 extracted single-rooted human teeth were instrumented with step-back technique to master apical file (MAF) size 35. Specimens were randomly divided into 2 groups (n = 20). In group 1, the specimens were treated with calcium hydroxide intracanal medication and in group 2, the samples did not receive any medication. The teeth were incubated in 100% humidity at 37°C for one week. After that, calcium hydroxide was removed using irrigation with normal saline and reaming with MAF. The root canals were obturated with gutta- percha and AH26 sealer using lateral compaction technique. Specimens were incubated in 100% humility at 37°C for 72 hours and then immersed in India ink for 1 week. Finally, the teeth were cleared and the maximum linear dye penetration was measured under a stereomicroscope at 4X magnification. The data were analyzed by T-test and Chi-square.
Results: There was no significant difference between the two experimental groups (P=0.068).
Conclusion: The findings of this study indicated that using calcium hydroxide as an intracanal medicament did not influence the apical microleakage after final obturation of the root canal system.

Background and Aims: The evaluation of absorbed dose received by patients could give useful information for radiation risk estimation. This study was performed to compare the entrance skin dose received by patients in cone beam computed tomography (CBCT), conventional and spiral computed tomography (CT).
Materials and Methods: In this experimental study, 81 calibrated TLD chips were used. the TLD chips were placed on facial, thyroid and end of sternum skin surface in patients referred for CT of the paranasal sinuses (3 TLD chips for each area) to estimate the absorbed dose received by central part of radiation field, thyroid and out of field areas, respectively. The data were analyzed using one-way ANOVA and Tukey tests.
Results: The dose delivered to the center of irradiated field was about 0.79±0.09 mGy in CBCT technique compared with 16.31±3.71 and 18.84±4.12 mGy for spiral and conventional CT, respectively. The received dose by the out of field areas was about 54 percent of central area dose. There was statistical significant relationship between the imaging modalities and absorbed dose received by patients (P=0.016). The least absorbed dose was for CBCT and the greatest dose was for conventional CT imaging technique.
Conclusion: The dose delivered to central area of irradiated field in conventional and spiral CT imaging modalities was about 24 times greater than of that in CBCT. Also, the highest received dose was for central area of radiated field and the lowest dose was for the out of field areas.

Background and Aims: Non-steroidal anti-inflammatory drugs (NSAIDs) are the most common drugs prescribed for controlling and post root canal treatment pain. During the last decade, a new generation of NSAIDs has been introduced such as Celecoxib and Gelofen with less gastrointestinal side effects and more analgesic effect. No studies have been performed to compare Celecoxib and Gelofen with other NSAIDs considering the reduction of post-endodontic pain therefore, this study was designed.
Materials and Methods: In this randomized double blind clinical trial study, 90 patients were divided into 3 groups and underwent root canal therapy. Celecoxib, Gelofen, or placebo was randomized prescribed to the patients 1 hour before treatment. The intensity of pain was recorded using visual analog scale (VAS) at 4, 8, 12, 24, 48 hours after completion of root canal treatment. The data were analyzed by means of repeated measurements, multiple comparisons and one-way ANOVA tests using SPSS software. P<0.05 was considered as the level of significance.
Results: The results showed significant difference between Celecoxib and Gelofen in comparison with placebo at 8 and 12 hours after initiation of treatment. There was no significant difference among three groups at 4, 24, and 48 hours after initiation of treatment.
Conclusion: According to the results, use of Gelofen or Celecoxib before treatment reduces post-endodontic pain. These drugs can be prescribed before initiation of treatment as the effective agents for reduction of post-endodontic pain

Background and Aims: The bond strength between veneer ceramics and zirconia frameworks is the weakest component in the layered ceramics. Due to the possible effect of adding pigments to the core materials on the bond strength between core and veneer as well as the introduction of new ceramic materials in dentistry, the aim of this study was to compare the zirconia core-veneer microtensile bond strength using two ceramic veneers with or without coloring the core.
Materials and Methods: In this experimental study, 12 disc-shaped specimens were fabricated using a manually aided design- manually aided manufactured (MAD/MAM) zirconia core (Zirkonzahn). Two veneering ceramics of Ceram Kiss and Zirkonzahn ICE were also used to veneer the specimens. Half of the Zirkonzahn discs were remained white and the others were colored by shade A2.Then, the discs were cut into microbars (30 for each group) and the microtensile bond strength of the core-veneer was calculated. The specimens were assessed using scanning electron microscopy (SEM) and the data were analyzed using two-way ANOVA and Student's t-test.
Results: Significant differences with respect to veneer layer were found (P<0.001). No significant differences were seen among colored and uncolored cores (P=0.69).
Conclusion: According to the apparent effect of veneering ceramics on the core-veneer bond strength, careful selection of these agents is essential to achieve adequate bond strength between core and veneer to prevent delaminating and chipping failures of zirconia veneered restorations.

Background and Aims: Lasers with different characteristics have been used to stimulate orthodontic tooth movement. Considering the contradictory findings in this regard, this study was designed to assess the effect of low level laser therapy (LLLT) on the rate of orthodontic tooth movement.
Materials and Methods: In this randomized clinical trial study, 12 patients (4 boys and 8 girls average age:16.9 ± 3.4) with extracted upper first premolars and required canine retraction into extraction site were included. While in both sides canines were retracted by NiTi coil spring, one side was exposed to GaAlAs laser (890 nm). LLLT was done (on the buccal and palatal mucosa by slow movement of probe) at the beginning of the first month. Impression and cast fabrication performed at the beginning of retraction, one and two months later. The amount of retraction on the cast was measured with the aid of a reference plaque fabricated on the rogae using a digital caliper. Data were analyzed using paired sample T-test and one-sample Kolmogorov-Simirnov test.
Results: There was no significant difference in the amounts of canine movement between laser exposed and control sides (P>0.05).
Conclusion: The energy dose of laser used in this study (72 J per each tooth) was not appropriate for increasing dental movement.

Background and Aims: Instrumentation of the root canals results in formation of smear layer which covers the dentinal tubules. In infected teeth, it is ideal to achieve a material that has the ability to remove the smear layer besides antimicrobial activity. Therefore, this study was designed to evaluate the antimicrobial effect of Citrus aurantifolia extracts (lime juice and rind extract) on Enterococcus faecalis within dentinal tubules in the presence of smear layer.
Materials and Methods: One-hundred and forty dentin tubes were prepared from bovine incisors. After removal the smear layer, the specimens were infected with Enterococcus faecalis. Then, the smear layer was reformed. Test solutions were used as the irrigants in study roups as follows: group 1: 5.25% NaOCl group 2: 17% EDTA group 3: NaOCl+EDTA group 4: Lime juice group 5: ethanolic rind extract of C.aurantifolia group 6: 96% ethanol. Dentin chips were collected from inner and outer layers of dentinal walls and optical density was measured. The data were analyzed using one-way ANOVA and Tamhane tests.
Results: In outer layer of dentin, the efficacy of rind extract was less than that of NaOCl+EDTA (P<0.05). Also Lime juice was less effective than EDTA, NaOCl and NaOCl+EDTA (P<0.05). In inner layer of dentin, Lime juice was significantly less effective than NaOCl and NaOCl+EDTA (P<0.05). The efficacy of rind extract was less than that of NaOCl+EDTA (P<0.05).
Conclusion: In the presence of smear layer, the antimicrobial activity of Lime juice was less than that of NaOCl but the efficacy of rind extract was similar to that of NaOCl.

Background and Aims: Marginal seal in class V cavities and determining the best restorative material to decrease microleakage is of great importance in operative dentistry. The aim of this study was to evaluate the effect of polishing time on the microleakage of three types of tooth-colored restorative materials in class V cavity preparations and to assess the marginal integrity of these materials using scanning electron microscope (SEM).
Materials and Methods: In this in vitro study, class V cavity preparations were made on the buccal and lingual surfaces of 30 bovine incisors (60 cavities). The specimens were divided into three groups each containing 10 teeth (20 cavities): group 1, Filtek Z350 (nanocomposite) group 2, Fuji IX/G Coat Plus (CGIC) and group 3, Fuji II LC (RMGI). In each group, half of the specimens (n = 20) were finished/polished immediately and the rest of them were finished/polished after 24 hours. All the specimens were thermocycled for 2000 cycles (5-50 °C). Epoxy resin replicas of 12 specimens (2 restorations in each subgroup) were evaluated using SEM and the interfacial gaps were measured. Finally, the teeth were immersed in 0.5% basic fuchsin dye for 24 hours at room temperature, sectioned and observed under stereomicroscope. The data were analyzed using Kruskal-Wallis and Mann-Whitney U tests and the comparison between incisal and cervical microleakage was made with Wilcoxon test.
Results: Incisal and cervical microleakage were not affected by polishing time in none of the three restorative materials (P>0.05). Cervical microleakage only in Fuji IX with immediate polishing was significantly higher than incisal microleakage (P<0.05). Incisal and cervical microleakage with immediate or delayed polishing were not significantly different in Fuji IX, Fuji II LC, and Z350 (P>0.05).
Conclusion: Immediate polishing is recommended in tooth-colored class V restorations.

Background and Aims: Relative displacement of the implant with respect to bone and quality of bone-implant contact play critical roles in the dental implant stability. The goal of this study was to investigate the dental implant stability using non-linear finite elements method. Therefore, bone-implant relative displacement due to applied force to the implant was calculated, and then an appropriate factor for defining quality of bone-implant contact was presented.
Materials and Methods: In order to develop a three dimensional model and compare the results with clinical studies, computed tomography (CT) scan data of a rabbit tibia was considered as a base. The model was exported to ABAQUS 6.9-1 to be analyzed using nonlinear finite elements method. Dynamic analysis was done on the model using the proper boundary condition and dynamic loads.
Results: Force-displacement curves in bone-implant interface were nonlinear. Friction coefficient, which is a criterion for implant stability and relative displacement, approximately became doubled as the vertical contact force was halved. However, the friction coefficient decreased with reduction of coulomb frictional coefficient.
Conclusion: Friction coefficient, which is calculated upon force-displacement curves, could be considered as a criterion to evaluate the dental implant stability. Decrease of the vertical contact force and also using rough surfaces improved the quality of bone-implant contact and stability of dental implant.

Background and Aims: Teaching of clinical skills is one of the most important aspects in training medical students. The aim of this study was to compare the effectiveness of videotaped and live demonstrations for applying fissure sealant on permanent molar teeth at Zahedan University of Medical Sciences (2010-2011).

Materials and Methods: Thirty 8th-semester dental students were randomly assigned in two groups (n = 15). In group 1, fissure sealant placement was taught using live demonstration while, in group 2, education was performed by videotaped demonstration. Then, each student applied fissure sealant on the patient's permanent molar tooth. The procedure of fissure sealant placement was assessed and scored by a pedodontist. The students were subsequently asked to rate how helpful the videotape or live demonstration had been. The data were analyzed using T-test and Chi-square test.

Results: The mean students' scores for videotaped and live demonstration were 16.5 and 16.53, respectively. There was no significant difference between the teaching methods according to students' scores (P>0.05). 33% of students rated videotaped demonstration as "good" for its helpfulness and 66% as "very good". However, live demonstration was rated as "good" and "very good" by 66% and 33% of students, respectively. There was no significant difference between two groups (P>0.05).

Conclusion: Both teaching methods were equally effective. Therefore, videotaped demonstration can be used in combination with live demonstration or as an alternative for it.

Background and Aims: Evaluation of the training is a principle for successful education. This study evaluated the effect of the theoretical course of community oral health on knowledge, attitude and self-reported practice of preventive dentistry in dental students at Tehran University of Medical Sciences and assessed the students' satisfaction from the course.

Materials and Methods: The intervention group comprised the forth-year students of Tehran University of Medical Sciences, Dental School and the controls were the forth year students of Shahid Beheshti Medical University. The questionnaire included questions on backgrounds, knowledge, attitude and practice of students towards preventive dentistry, and students' satisfaction from the course. The intervention was 17 weekly sessions (hours) of the community oral health course. The sum of scores for questions on backgrounds, knowledge, attitude and practice were calculated and changes were analyzed by General Linear Model.

Results: The mean baseline knowledge score in the intervention group was 16.9 (SD=4.0) and for the control group 16.3 (SD=3.5). Corresponding figures were 23.7 (SD=3.4) and 17 (SD=3.3) after intervention. General linear model showed the intervention to be successful in increasing the students' knowledge scores on preventive dentistry (P<0.0001). Most students (92%) were satisfied with learning several new items during the course.

Conclusion: The students' knowledge was successfully increased by passing the theoretical course of community oral health delivered by teacher-centered method of lecture. Student-centered methods may help in changing the students' attitude and practice of preventive dentistry.

Background and Aims: A large number of investigations have revealed that physical and chemical alterations and weight loss could occur in composite materials exposed to acidic phosphate fluoride (APF) gel. The purpose of this study was to assess the microleakage of a fissure sealant and a flow composite exposed to acidulated phosphate fluoride (APF) gel.

Materials and Methods: In this in vitro study, 60 intact human extracted premolar teeth were used. After preparing the occlusal groove, the teeth were divided into two groups (n=30). Teeth were filled with either Helioseal fissure sealant (Vivadent, Germany) or Tetric flow composite (Vivadent, Germany). After that, each group was divided into two subgroups (n=15): 1.23% APF gel (Sultan, U.S.A) was applied in the case subgroups, while control subgroups were preserved in normal saline solution. All of teeth were covered with 2 layers of nail varnish except for the filling zone and 1mm around the border of filling. After submerging in 0.5% fushin solution, specimens were sectioned bucco-lingually. Then dye penetration through the filling and fissure sealant was assessed by means of a stereo-microscope. The depth of dye penetration was scored. The data were analyzed using One-way ANOVA and Levene test.

Results: The mean values of dye penetration were 1.26±1.09, 1.4±1.05, 1.2±1.37, and 1.4±1.35 for fissure sealant+gel, composite+gel, composite+normal saline, and normal saline groups, respectively. No significant difference was found in inter-groups (P=0.96).

Conclusion: Considering the result of the present research, APF gel had no significant effect on the microleakage of Tetric flow composite filling and Helioseal fissure sealant and thus, it can be applied for routine usage.

Background and Aims: The seal provided by a root canal filling may be compromised by post space preparation. The aim of this study was to compare coronal microleakage around cast posts and prefabricated pins.

Materials and Methods: Thirty-eight extracted single-rooted, maxillary human teeth were prepared with step back method and filled with gutta-percha and sealer. After removing 8 mm of coronal gutta-percha with peeso-reamer drills #2 in twenty canals, the teeth were divided into three groups: cast post & core, prefabricated pin,  and gutta-percha (without any post or pin). After thermocycling, the samples were immersed in India ink for seven days. Finally, the specimens were cleared and the coronal microleakage of dye was then measured by the stereomicroscope. The data were analyzed by One-way ANOVA and Tukey HSD tests.

Results: According to the results, the mean dye penetration in cast posts, pins, and group without post or pin was 3.3 (0.39), 5.2 (0.47), and 7.2 (0.15) mm, respectively. There was no statistically significant difference between the groups (P>0.05).

Conclusion: Considering the importance of adequate filling of crown for prevention of microleakage, the method for coronal filling must be selected with caution. According to the results of this study, no significant difference between microleakage of cast posts and prefabricated pins was observed.

Background and Aims: Composite restorative materials and dental adhesives are usually cured with light sources. The light direction may influence the bond strength of dental adhesives. The aim of this study was to evaluate the effect of light direction on the microtensile bond strength of fifth and sixth generation dental adhesives.

Materials and Methods: Prime & Bond NT and Clearfil SE bond were used with different light directions. Sixty human incisor teeth were divided into 4 groups (n=15). In groups A and C, Clearfil SE bond with light curing direction from buccal was used for bonding a composite resin to dentin. In groups B and D, Prime & Bond NT with light curing direction from composite was used. After thermocycling the specimens were subjected to tensile force until debonding occurred and values for microtensile bond strength were recorded. The data were analyzed using two-way ANOVA and Tukey post hoc test.

Results: The findings showed that the bond strength of Clearfil SE bond was significantly higher than that of Prime&Bond NT (P<0.001). There was no significant difference between light curing directions (P=0.132).

Conclusion: Light curing direction did not have significant effect on the bond strength. Sixth generation adhesives was more successful than fifth generation in terms of bond strength to dentin.

Background and Aims: The aim of this study was to evaluate the quality of an experimental hydrofluoric acid (HF) for preparation of porcelain and to compare it with two commercial hydrofluoric acids in Iranian trademark.

Materials and Methods: A- Evaluation of etch pattern of experimental HF using scanning electron microscope (SEM): 6 feldespathic discs were divided into 3 groups. Each group was etched with related HF (experimental, Ultradent and Kimia) for 1 minute. SEM images were recorded at 3 magnifications. B- Bond strength test: 18 feldespathic discs were considered for each acidic group. Then the porcelain surfaces were etched and bonded to composite with unfilled resin. Consequently, the microshear test was done. C- Microleakage test: 54 discs were divided into 3 groups (n=18). Then the porcelain surfaces were etched and bonded to composite with unfilled resin and finally observed under stereomicroscope. The data were analyzed with one-way ANOVA and Smirnov tests.

Results: SEM analysis showed no difference between groups in terms of etch pattern. Microshear bond strength values for experimental, Kimia, and Ultradent HF were 28.53 (±4.92), 28.21 (±6.61), and 26.14 (±7.61) MPa, respectively. There was no significant difference between the bond strength of test groups (P<0.05). Furthermore, no significant difference was found between the microleakage of test groups (P>0.05).

Conclusion: Quality of experimental HF in terms of etch pattern, microshear bond strength and microleakage of composite/porcelain interface was similar to that of two commercial hydrofluoric acids.

Background and Aims: Reliable bonding between tooth substrate and zirconia-based ceramic restorations is always of great importance. The laser might be useful for treatment of ceramic surfaces. The aim of the present study was to investigate the effect of laser irradiation on the shear bond strength of zirconia ceramic surface to dentin.

Materials and Methods: In this experimental in vitro study, 40 Cercon zirconia ceramic blocks were fabricated. The surface treatment was performed using sandblasting with 50-micrometer Al2O3, CO₂ laser, or Nd:YAG laser in each test groups. After that, the specimens were cemented to human dentin with resin cement. The shear bond strength of ceramics to dentin was determined and failure mode of each specimen was analyzed by stereo-microscope and SEM investigations. The data were statistically analyzed by one-way analysis of variance and Tukey multiple comparisons. The surface morphology of one specimen from each group was investigated under SEM.

Results: The mean shear bond strength of zirconia ceramic to dentin was 7.79±3.03, 9.85±4.69, 14.92±4.48 MPa for CO₂ irradiated, Nd:YAG irradiated, and sandblasted specimens, respectively. Significant differences were noted between CO₂ (P=0.001) and Nd:YAG laser (P=0.017) irradiated specimens with sandblasted specimens. No significant differences were observed between two laser methods (P=0.47). The mode of bond failure was predominantly adhesive in test groups (CO₂ irradiated specimens: 75%, Nd:YAG irradiated: 66.7%, and sandblasting: 41.7%).

Conclusion: Under the limitations of the present study, surface treatment of zirconia ceramics using CO₂ and Nd:YAG lasers was not able to produce adequate bond strength with dentin surfaces in comparison to sandblasting technique. Therefore, the use of lasers with the mentioned parameters may not be recommended for the surface treatment of Cercon ceramics.

Background and Aims: Disc displacement is the most common temporomandibular joint disorder and magnetic resonance imaging (MRI) is the gold standard in its diagnosis. This disorder can lead to changes in signal intensity of magnetic resonance (MR). The purpose of this study was evaluation of correlation between relative signal intensity of MR images of retrodiscal tissue, superior and inferior head of lateral ptrygoid muscle with type of anterior disk displacement and condylar head flattening in patients with temporomandibular disorder (TMD).

Materials and Methods: In this retrospective study, 31 MR images of patients who had anterior disc displacement were evaluated. After relative signal intensity measurement for retrodiscal tissue, superior and inferior head of lateral ptrygoid muscle, the correlation between relative signal intensity and type of anterior disc displacement was evaluated with repeated measure ANOVA test. In each of these 3 areas, t-test was used to compare the groups with and without condylar head flattening.

Results: The correlation between relative signal intensity of MR images and type of anterior disc displacement in retrodiscal tissue, superior and inferior head of lateral ptrygoid muscle was not significant. There was also no statistically significant correlation between relative signal intensity of MR images and flattening of condylar head in retrodiscal tissue, superior and inferior head of lateral ptrygoid muscle (P>0.05).

Conclusion: According to findings of this study, relative signal intensity of MR images in retrodiscal tissue, superior and inferior head of ptrygoid muscle is not a good predictor for type of anterior disc displacement and flattening of condylar head. It seems that this cannot be used as a diagnostic marker for TMD progression.

Dentistry has been a field dominated by a constant improvement of synthetic biomaterials. Tissue engineering of tooth is coming to change the panel of the dental materials such as restorative materials and implants. Certainly, it is the largest transition in history of dental materials science in terms of accepting this new and exciting technology. The objective of this article is to present various implications of tissue engineering in different fields of dentistry. To achieve this goal, a review of the literature was carried out by using Medline database to search topics including "dental stem cells", "teeth tissue engineering", "regenerative dentistry", "oral surgery", "periodontal regeneration" and "regenerative endodontics". These searches were limited to articles published after the year 2000. On the basis of our literature review, we have found that although there are significant challenges in oral tissues engineering, engineered tissues will find many applications in dentistry within the next few years.

Background and Aims: Since a large marginal opening allows more plaque accumulation, gingival sulcular fluid flow and bone loss, microleakage, recurrent caries and periodontal disease, marginal fit is of great importance in fixed restorations. The aim of this study was to compare the marginal fit of zirconium-based all ceramic (Cercon) frameworks, made by two different approaches (Direct and Wax-up scanning).

Materials and Methods: An abutment analog, with 5.5 mm width and height, was selected as an experimental model. Twenty all ceramic cores were made for this model (10 specimens per each group). In the first group (Direct), experimental models were scanned directly using Computer Aided Design/Computer Aided Manufacturing (CAD/CAM). The core was then designed with the aid of a computer and machined. In the second group (Wax-up or CAM) a wax-up pattern was made on the experimental model and then scanned and milled from Cercon blocks. The marginal discrepancy of each specimen was recorded using a stereomicroscope (SZX9, Olympus, Japan) and digital camera (Mode TK, C1380E, JVC, Japan) and then measured by Adobe Photoshop CS software. The data were analyzed using T-test.

Results: Mean marginal discrepancy of Direct group (85.2±3.95 µm) was significantly less than that of Wax-up group (120.2±6.91 µm).

Conclusion: Improved marginal fit of zirconia ceramics was found with the direct model scanning compared with that of Wax-up scanning using CAD/CAM.

Background and Aims: Since impression materials usually contact with saliva, blood, and oral soft tissues, their microbial contamination are harmful in immunocompromised patients. The aim of the present study was to determine the bacterial and fungal contamination in common impression materials.

Materials and Methods: In current lab trial study, 5 different samples from each 4 impression materials were homogenized in 1 ml Tween 80 and then 100µl of each sample were cultured onto blood agar, EMB, or sabouraud dextrose agar. Bacterial and fungal cultures were incubated at 37º C and 30º C, respectively. The isolated bacterial and fungal colonies were enumerated and identified using specific diagnostic media and tests. Data were analyzed using Kruskal-Wallis test.

Results: Totally 75% of samples had one or several bacterial contaminations. Iranian alginate and Speedex (putty) were the most contaminated samples. On the other hand, Speedex (light body) and foreign alginate showed lower contamination. Species of Micrococcus, Staphylococcus, Bacilluses, Corynebacteria, gram negative Citrobacter, Actinomycetes and Neisseria were isolated from the analyzed impression materials. Aspergillus, Penicillium, Alternaria, Cladosporium and Sepdonium were the fungi isolated from impression materials. Statistical significant difference was shown between bacterial contamination of Iranian and foreign alginates (P=0.001). There was no statistical significant differences between the bacterial and fungal isolated colonies (CFU/gr) of 4 tested impression materials (P=0.21).

Conclusion: Several opportunistic bacteria and fungi were isolated from impression materials especially from Iranian alginate and Speedex putty which indicated their contamination.