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Showing 3 results for Ameloblastoma
Immunohistochemicai study of Ki- 67 expression in unicystic Ameloblastoma and Dentigerous cyst
M. Eslami , N. Eshghyar , F. Tirgari , G. Rezvani ,
Volume 17, Issue 1 (4-2004)
Abstract
Statement of Problem: Differentiation of dentigerous cyst from unicystic ameloblastoma, discovering any initial ameloblastic changes in lining epithelium of dentigerous cyst at early stage, and differentiation between hyperplastic odontogenic epithelium in fibrous capsule of dentigerous cyst from ameloblastic proliferation, need to an accurate and reliable technique.
Purpose: The aim of this study was to determine and compare Ki-67 immunoreactivity in various locations of the epithelium of Dentigerous cyst and Unicystic Ameloblastoma.
Materials and Methods: In this historical Cohort study, 15 cases of dentigerous cyst and 9 cases of unicystic ameloblastoma were selected. Immunohistochemistry staining was performed by M1B-1 (murine monoclonal antibody against Ki-67). The stained nucleous were counted in basal and suprabasal layer of lining epithelium of both lesions in 3000 epithelial cells. Finally, the percentage of positive cells (presented as labeling index) was calculated, t- student test was used to analyze the related data.
Results: Ki-67 (LI) in basal layer of Dentigerous cyst (2.59±1.66) and Unicystic Ameloblastoma (3.76±79) had no significant differences, but Ki-67 (LI) in suprabasal layer of unicystic ameloblastoma (2.15±0.69) was significantly higher than dentigerous cyst (0.77±0.55) P=0.003).
The difference between the average numbers of positive cells for Ki-67 (LI) in these two lesions was statistically significant (P<0.05) and it was higher in Unicystic Ameloblastoma than Dentigerous cyst.
Conclusion: Based on the findings of this study, it is suggested that Ki-67 (LI) in suprabasal layer or throughout the epithelium can be considered as a useful marker for differential diagnosis between dentigerous cyst and unicystic ameloblastoma.
Immunohistochemical evaluation of p21 and cyclin D1 expression in ameloblastoma of the jaws
M. Khalili , M. Eslami , P. Masoumi ,
Volume 20, Issue 1 (5-2007)
Abstract

Background and Aim: The cell cycle is an important event in tumor growth and differentiation and several molecules are involved in this process. The aim of this study was to evaluate the expression of cyclin D1 (a cell cycle inducer) and p21 (a cell cycle inhibitor) in ameloblastoma of the jaws.

Materials and Methods: In this cross-sectional study, 40 cases of ameloblastoma were selected from the archive of oral pathology department. 3 micron sections were cut from paraffin blocks and immunohistochemically stained with antibody against cyclin D1 and p21waf. Stained cells were counted using an eyepiece graticule and labeling index was calculated. Data were analyzed by SPSS version 11.5 for windows using Mann-Whitney and Wilcoxon signed ranked tests with p<0.05 as the level of significance.

Results: Expression of cyclin D1 protein was detected in nuclei of many tumoral cells. The expression of cyclin D1 in solid and unicystic ameloblastoma and also between its follicular and plexiform variants was not statistically different (P>0.05). There was no statistically significant difference in expression of cyclin D1 between peripheral and central cells (P>0.05). Expression of p21 protein was detected in nuclei of some tumoral cells. There were no statistically significant differences between p21 expression in unicystic and solid ameloblastoma (P>0.05). P21 expression was statistically different between plexiform and follicular variants of ameloblastoma (P=0.049). The difference between p21 expression in peripheral cells of plexiform and follicular variants was statistically significant (P=0.009). This was not observed in central cells. There was no statistically significant relation between p21 and cyclin D1 expression in ameloblastoma (P>0.05).

Conclusion: Based on the results of this study, cyclin D1 expression in ameloblastoma is in high level and it could have an important role in the process of tumorigenesis. P21 expression in ameloblastoma is very faint and its possible effects need further investigation.


Comparison of cathepsin-D expression in unicystic ameloblastoma, odontogenic keratocyst and orthokeratinized odontogenic cyst using an immunohistochemical method
Forooz Keshani, Neda Kargahi, Maedeh Omani,
Volume 35, Issue 0 (5-2022)
Abstract
Background and Aims: Cathepsin-D is a well-known protease that promotes invasion in tumoral lesions. Considering the cystic neoplasm nature of odontogenic keratocyst (OKC), the aim of this study was to compare the expression of cathepsin-D in this lesion with the unicystic ameloblastoma (UA) and orthokeratinized odontogenic cyst (OOC) for better understanding of its behavior.
Materials and Methods: In this descriptive-analytical study, we used paraffin blocks available in the archives of oral and maxillofacial pathology department of dental school (8 unicystic ameloblastoma (UA), 8 odontogenic keratocyst (OKC) and 8 orthokeratinized odontogenic cysts (OOC)) which they were stained immunohistochemically with cathepsin-D. Then, the samples were observed simultaneously by two oral pathologists for detection the intensity and pattern of epithelial and stromal cells staining. Data were analyzed by SPSS20 and Kruskal-Wallis, Mann-Whitney and Chi-square test (P<0.05).
Results: The staining intensity of the epithelial cells of UA group was significantly more than OOC and OKC (P=0.02). The staining intensity of the stromal cells of UA was more than the other two groups, although this difference was not statistically significant (P=0.32). The pattern of cell staining in epithelium and stroma did not show any significant difference between the three groups in this study (P=0.15, 0.22).
Conclusion: The results of this study regarding the intensity expression of cathepsin-D in these three odontogenic lesions could be considered as a probable evidence for the new odontogenic lesions classification (WHO2017) in terms of reintroducing OKC as an odontogenic cyst. If this idea is rejected, it seems that cathepsin-D expression is not associated with the invasive behavior of this cyst, and further investigation of other markers in the epithelium and stroma simultaneously is suggested for a better understanding of its biological nature.

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