Pooneh Mahmoudi, Kiumarss Amini, Parviz Amini,
Volume 32, Issue 1 (7-2019)
Abstract
Background and Aims: Candida is an opportunistic pathogen that causes illness in people with a defective or weakened condition. Infectious diseases (periodontal diseases) are inflammatory and malignant inflammation of the dental-gum complex, in which the growth of biofilms caused by Candida glabrata, Parapseilosis and Tropikalis is less than Candida albicans. Brown algae Sargasum is more compatible with human medicines due to having a natural origin than chemical drugs and has less side effects. The aim of the present study was to investigate the molecular characteristics of Candida species isolated from periodontal infections and the effect of Sargassum glaucescens extract on biofilm gene expression using Real-Time-PCR.
Materials and Methods: Oral samples of periodontal infection were collected from the referred patients. To isolate the candidate species, the specimens were cultured on a Sabouraud dextrose agar containing chloramphenicol (SDAc). The extracted DNA was extracted from colonies grown from Kit and Glass pearl. Grown chickens were identified by specific primers by PCR-RFLP method. In order to detect the expression of als genes in Candida isolates, RNA extraction was performed using Phenol-Chloroform and Pearl glass, and the CLSI-M27-A2 method was used to evaluate the effect of Sargasum glaucescens extract of algae.
Results: The results showed that the expression of als gene in periodontal infection is higher than other genes. Another role is als in the formation of Candida albicans biofilm. The minimum inhibitory concentration of fungal growth was 256 μg/ml by algae extract. Sargasum glaucescens reduced the expression of als gene expression by about 62% in the sample.
Conclusion: Sargasum glaucescens algae possesses specific pharmacological properties and antimicrobial and antimicrobial effects. The results of the study using Real Time PCR showed that expression of als gene in isolated studied with Sargasum algae extract was lower than untreated isolates. Thus, this indicated the positive role of treatment by sargasum glaucescens extract in reducing the expression of biofilm gene in isolates.
Fatemeh Hemmati, Mansour Bayat, Kumarss Amini,
Volume 34, Issue 0 (5-2021)
Abstract
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method.
Materials and Methods: For this study, 100 individuals including 50 patients and 50 healthy individuals were recruited and examined by a periodontist. After identifying sulfate-reducing bacteria by biochemical tests and specific media, the effect of aloe vera extract on them was investigated and the expression of BFR gene against housekeeping gene (16srDNA) was determined by Real time- PCR test via T-test analysis method.
Results: The data showed that 12 strains of sulfate-reducing bacteria were isolated from the samples, 5 of which had BFR gene and their gene expression was significantly reduced by aloe vera gel (P<0.05). |
Conclusion: The data of this study in proving the anti-biofilm and antibacterial effects of aloe vera extract showed that the expression of the target gene is reduced. It seems that this substance can be used as an alternative to oral hygiene chemicals.
Background and Aims: Due to the increasing problems and side effects of the use of chemical antibacterial agents as well as antibiotic resistance, this study aimed to evaluate the effects of aloe vera gel on biofilm gene expression of sulfate-reducing bacteria (SRB) isolated from patients with periodontal infection by Real time-PCR method.
Materials and Methods: For this study, 100 individuals including 50 patients and 50 healthy individuals were recruited and examined by a periodontist. After identifying sulfate-reducing bacteria by biochemical tests and specific media, the effect of aloe vera extract on them was investigated and the expression of BFR gene against housekeeping gene (16srDNA) was determined by Real time- PCR test via T-test analysis method.
Results: The data showed that 12 strains of sulfate-reducing bacteria were isolated from the samples, 5 of which had BFR gene and their gene expression was significantly reduced by aloe vera gel (P<0.05). |
Conclusion: The data of this study in proving the anti-biofilm and antibacterial effects of aloe vera extract showed that the expression of the target gene is reduced. It seems that this substance can be used as an alternative to oral hygiene chemicals.
