Journal of Dental Medicine

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Due to the differences in the composite and morphology of dentin in primary and permanent teeth, it is necessary to make improvements in bonding techniques to promote the strength of composite resins bonded to the dentinal surface, in primary teeth. The use of lower radiation, to make structural and chemical changes in dentinal surfaces has been investigated. This research was conducted to evaluate the shear strength of the composite bonded to primary teeth dentin after Nd: YAG laser radiation and acid etching for conditioning. Peripheral dentin of the buccal and lingua! surfaces of 60 extracted posterior primary teeth were exposed and polished with 600 grit with Sic paper. The teeth were divided randomly in 3 groups of 20 teeth. In group 1 etching gel, primer and adhesive of scotch bond multipurpose system (SMP), in group 2 laser at 1.6 w and 80 mj/pulse, and in group 3 laser at 2 s and 700 mj/pulse were used. Moreover, in groups 2 and 3, after laser radiation, acid etching, primer and adhesive of SMP system were applied. After necessary laboratory tests, the mean shear bond strength in MPa were 20.99±5.3 (group 1), 23.82±6.31 (group 2) and 26.58±5.59 (group 3). ANOVA, scheffe, tukey statistical tests showed that the bond strengths of group 3 were statistically higher than group 1. The frequency of dentin cohesive failures were significantly higher in groups 2 and 3, compared to group 1 that indicates a higher bond strength in these groups. Scanning electron mirographs of laser radiated surfaces, show a porous and rough surface morphology that enhances the mechanical bond of the composite.

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Dentine bonding systems are usually unfilled, and so their shrinkage may be significant. High shrinkage may cause internal stress at the interface between resin-composite restoration and the dentine substrate. Failure of the adhesive interface may be observed due to the interna! stress. The aims of this study were:A) To obtain a suitable method for measuring the kinetics of polymerisation shrinkage in unfilled resm at different thicknesses, particularly for thin films.B) Consideraing the effect of thickness on shrinkage.Scotchbond Multipurpose (3M) adhesive bond resin was used. To overcome the particular challenges presented by thin films, a filled-ring measurement procedure was used. Also, a non-contact laser analogue displacement sensor system was developed and applied to measure polymerisation shrinkage. Regression analysis was performed on a complete data set. Non-linear regression analysis established a logarithmic relationship between polymerisation shrinkage and layer thickness. The method applied in this study was found to be sensitive and accurate procedure for determining photo-polymerisation shrinkage of thin films. Polymerisation shrinkage increased with logarithmic of the adhesive thickness.

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Dentin bonding agents create stronger bonding between dental composites and dentin. But, none of them can prevent the microleakage. The important factors of progressing microleakage are the stress of polymerization shrinkage of resin composite and removal of smear layer in total-etch technique. The aim of this study was the evaluation of the effect of experimental method (modifying smear layer and etching of enamel margin) and comparison with the total-etch technique. In this experimental method, 60 extracted human molar teeth were disinfected. Then, class 5 cavities were prepared on the buccal surface and 1 mm above CEJ. The specimens were divided randomly to 6 groups with iO samples in each group. The specimens groups were:
1- Scotchbond Muiti- Purpose Plus Adhesive System (S.B.M.P.P) +Tetric composite
2- S.B.M.P.P + Ariston composite
3- Ariston liner + Ariston composite
4- S.B.M.P.P without enamel and dentin etching + Tetric composite + rebonding with Flowable Tetric
5- S.B.M.P.P without enamel and dentin etching + Ariston composite + rebonding with Flowable Tetric
6- S.B.M.P.P without enamel and dentin etching + Tetric composite
After finishing and polishing, the specimens were termocycled, stained with fushin, sectioned within the specimens and evaluated for dye penetration with stereomicroscope. Wilcoxon Sign and Kruskal Wallis tests were used for statistical analysis.
Results showed that group 3 had microleakage more than groups of 1, 2 and 5 (P<0.0 5) and this difference was significant. There was no significant difference among the other groups.

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Statement of Problem: Considering that the role of collagen fibers in dentin adhesion has not been thoroughly established yet, the removal of exposed collagen fibers with a deproteinization agent such as sodium hypochlorite following etching may facilitate access of adhesive resins to a substrate that is more penetrable and less sensitive to water content which in turn would lead to a more durable bonding system. Furthermore, due to sodium hypochlorite clinical application as a cleanser or canal irrigator, its effects on the surface before etching may influence adhesive bonding strength.

Purpose: The goal of this study was to evaluate the effects of a two-minute 5.25% NaOCL application on composite restorations microleakage, using two different adhesive systems.

Materials and Methods: In this interventional experimental study, on seventy-two extracted boving incisors class V cavities were prepared on dentinal surfaces. The specimens were then randomly divided into six equal groups: A1) Acid etch (AE) dentin bonding Scontchbond Multipurpose Plus (SBMBP), A2) AE/ dentin bonding One Step (OS), B1) NaOCL/ AE/SBMPp, B2) NaOCL/AE/OS, C1) AE/NaOCL/SBMPp, C2) AE/NaOCL/OS. After bonding procedures according to the manufacturer’s instructions, cavities were restored using Z100 composite. Then, the specimens were thermocycled for 500 times in water baths of 5 and 55C. After thermocycling, the specimens were immersed in a 0.2% solution of basic fuchsin for 24 hours. A buccolingual section at the center of each restoration was obtained and examined with a stereomicroscope to evaluate color penetration into cavities. The data were subjected to two-way variance analysis.

Results: The microleakage of group B was significantly less than those of A and C (P<0.001). No significant difference was found between groups A and C (P=0.73). There were also no significant differences within groups A, B and C (P=0.852). No interaction was observered between dentin bondings and surface treatment (P=0.946).

Conclusion: The findings of this study revealed that NaOCL application after etching, for hybrid layer removal, did not make any difference on microleakage as compared with conventional bonding systems. However, its application before etching improved the seal of restoration. Additionally, there was not any significant difference between two different dentin bondings.

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Statement of Problem: Porcelain restorations are susceptible to fracture and a common method for repairing is the use of silane and composite on etched porcelain. Although HF is very effective in porcelain etching but has detrimental effects on tissues.

Purpose: In this study, the effect of APF and PHA was compared with HF in porcelain etching. Also the role of silane, unfilled resin and dentin bonding in bond strength of composite- porcelain was evaluated.

Methods and Materials: In this experimental in-vitro study, one-hundred twenty porcelain square blocks (552 mm) were prepared and bonding surfaces of each sandblasted. Samples were divided into three groups. The first group (n=40) were etched with buffered HF 9.5% (Ultradent) for 1 min., the second group (n=40) were etched with Iranian APF 1.23% (Kimia) for 10 minutes and the third group (n=40) were etched with Iranian PHA 37% (Kimia) for 1 min. Ultradent silane was applied on the surfaces of half of cases in each group. On the surfaces of half of silane-treated samples unfilled resin was applied and dentin bonding was used on the surfaces of the remaining. Samples without silane were treated in a similar manner. Composite cylinder with 4mm diameter and 2 mm height was bonded to porcelain. Specimens were stored in 37°C distilled water for 24 hours and subjected to 500 cycles. Shear bond strength was measured with an Instron machine and type of fracture was evaluated using a stereomicroscope. Results were analyzed using 3 way ANOVA, Kaplan- Maier and Tukey HSD tests.

Results: Findings showed that PHA and APF roughened the porcelain surface without creating retentive micro undercuts but HF etches porcelain and creates retentive microundercuts. Ultradent silane had no significant effect on bond strength of porcelain- composite. Unfilled resin with Ultradent silane compared with dentin bonding with the same silane is more effective in bond strength of composite- porcelain.

Conclusion: Based on present study, application of Ultradent silane on sandblasted and etched porcelain with PHA or APF cannot be used as an alternative to this silane on sandblasted and etched porcelain with HF.

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Statement of Problem: One of the greatest problems in metal –ceramic restorations is debonding of porcelain from dental alloys. Production of dental alloys by Iranian companies necessitates the evaluation of physical and handling properties of these products.

Purpose: In this study the bond strength between porcelain and two types of base metal alloys, Supercast (with beryllium) and Minalux (without beryllium) was investigated.

Materials and Methods: In this experimental study 10 cylindric bars from each base metal alloy were prepared. The bars were degassed and porcelain was applied around them in a disc form (8 mm diameter and 2 mm thickness). The bond strength of porcelain to metal bars was tested with the shear strength test by Instron. Data were analyzed with student t-test and P<0.05 was considered as the limit of significance.

Results: The mean failure load was 71.58±6.4 KgF for Supercast and 67.34±5.48 for Minalux alloy. The bond strength of Supercast and Minalux were 55.85±4.99 MPa and 52.54±4.27 MPa respectively. The difference was statistically significant (P0.001).

Conclusions: This study showed that nickel-chromium-beryllium alloy (Supercast) produced significantly better ceramometal bonding than nickel chromium alloy without beryllium (Minalux).

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Background and Aim: Although the use of adhesive systems can be effective in decreasing microleakge, it is still a major problem in composite resin restorations. The aim of the present study was to evaluate the marginal sealing ability of resin composite restorations using four dentin bonding systems in both primary and permanent teeth.

Materials and Methods: In this experimental study, one hundred and sixty extracted human teeth (80 primary and 80 permanent) were selected. All of the samples received a class V cavity preparation on the buccal surfaces (The coronal half in enamel and the gingival half in cementum or dentin). Each group was then divided into four subgroups each containing 20 teeth. Four different dentin bonding systems (Scotchbond Multipurpose, Single Bond, Clearfil SE Bond and Prompt L-Pop) were used in each subgroup. Then the cavities were filled with composite resin (Z100 for SBMP and Clearfil AP-X for Clearfil SE Bond). Samples were thermocycled, immersed in 0.5% basic fuschin, cut faciolingually and evaluated for dye penetration using a binocular stereomicroscope. Kruskal-Wallis and Dunn tests were used for comparison of microleakage between groups with p<0.05 as the limit of significance.

Results: The results showed that: There was significant difference in microleakage among four adhesive systems in both incisal and gingival margins of permanent teeth and in incisal margin of primary teeth (P=0.000, P=0.002, P=0.000 respectively). There was no significant difference in microleakage of restorations with each of four systems between permanent and primary teeth in both incisal and gingival margins except for PLP, which showed a significant different microleakage in the cervical margins (P=0.009). PLP showed better cervical seal in primary teeth compared to permanent teeth. Clearfil SE Bond showed acceptable results at incisal and cervical margins in primary teeth in comparison to other bonding systems.

Conclusion: Based on the results of this study, CSEB, SB and SBMP systems showed acceptable clinical results in primary and permanent teeth. Only PLP showed weak results in reducing microleakage. CSEB can be used successfully in primary teeth because of simplicity and reducing leakage in primary dentin and enamel.

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Background and Aim: An important requirement for a dentin bonding agent is biological compatibility. Since dentin bonding agents are placed in cavity preparations with subgingival extensions, with direct contact to gingival and mucosal tissues, tissue response to these materials must be investigated. The aim of this study was to examine the cytotoxicity of AdheSE, a self etching adhesive, on human gingival fibroblasts.
Materials and Methods: In this experimental in vitro study, primary human gingival fibroblasts were exposed to different dilutions of primer & bond of AdheSE (Vivadent, Liechtenstein). The toxicity of the primer was tested in 30 seconds, 300 seconds and 24 hours. The cytotoxicity of the bond was analyzed in uncured mode after 20 seconds, 5 minutes and 24 hours. In cured mode, tested materials were analyzed after 24 and 48 hours. Cytotoxic effects were evaluated using MTT, cell counting and DNA condensation assays. Data were analyzed by two way repeated measure ANOVA with p<0.05 as the level of significance.
Results: MTT Assay revealed that uncured AdheSE Bond was toxic only in 10-1 dilution and the difference with control group was significant (P<0.05). By increasing the time to 300sec. and 24h, dilutions of 10-2 and 10-4 were the most cytotoxic respectively. Cytotoxicity of uncured primer after 30 sec. and 300 sec. began from 10-2 and after 24h began from 10-2 and reached to 10-1. AdheSE in cured mode showed significant difference with control group in 1:2 (P<0.001),1:4 & 1:6 (P<0.01) dilutions. In cell counting assay only the 1:2 dilution was significantly more toxic than control group. Apoptosis (a morphological and biochemical distinct form of cell death that regulates cell turnover) comprised in less than 5% of total death in both cured and uncured adhesives.
Conclusions: Based on the results of this study, by increasing the exposure time, smaller amounts of bonding could be cytotoxic. Cytotoxicity was related to material, dilution, time of exposure and curing. It would be necessary to identify the toxic ingredients of this adhesive and replace them by more biocompatible components.

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Background and Aim: The use of fiber reinforced posts in endodontically treated teeth has become increasingly common. But their retention in root canals must be considered seriously. The aim of this study was to evaluate the effect of light-cure and dual-cure bondings on regional bond strength of a fiber composite post.

Materials and Methods: In this experimental in vitro study, 20 endodontically treated teeth were randomly divided into two groups. In the first group, a dual-cure bonding (Scotchbond Multi-Purpose Plus, 3M ESPE/USA) [SBMP] was used and in the other group, a light-cure bonding (Single Bond, 3M ESPE/USA) [SB] was applied according to the manufacturer's instructions. A dual-cure resin cement (Rely X ARC, 3M ESPE/USA) was used to cement the post (Glassix, Harald Nordin SA,Switzerland). Coronal 8mm of cemented posts were sectioned in equal thirds using a 0.1mm diamond disc. Each slice was polished by a soft and wet abrasive paper in order to get a 2mm thickness. Loading was performed by a testing machine (Zwick/Germany) at a speed of 1mm/min until the post was dislodged. Data were analyzed using one-sample Kolmogorov-Smirnov, T and ANOVA tests with P<0.05 as the level of significance.

Results: There was a significant difference between the two adhesive systems in the middle third of the canal block with higher bond strength in SBMP group (p=0.02). In SB group the bond strength of the cervical region was higher than the middle and apical thirds (p<0.05). In SBMP group, there was no statistically difference between bond strength of the three regions (p=0.117).

Conclusion: Based on the results of this study, dual-cure bonding could be recommended for composite post cementation into root canals, because its bond strength was more uniform in different regions of root and greater in the middle and third regions.

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Background and Aim: Application of varnish and dentin bonding agents can effectively reduce microleakage under amalgam restorations. Also rebonding may show some effects on microleakage and its complications. The aim of this study was to evaluate the effect of liner/ adhesives on microleakage of Class V amalgam restoration with or without rebonding.

Materials and Methods: In this in vitro study Class V cavities were prepared on sixty sound human maxillary premolars with the gingival floor 1mm below the CEJ. Cases were divided into six groups of ten teeth each. Specimens in group 1 and 2 were lined with Copalite and Scotchbond Multi-Purpose (SBMP) respectively. In the third group (control) no liner was applied. The teeth were then restored with spherical amalgam. Specimens in group 4 to 6 received the same treatments but after filling, the interfaces of restorations and teeth were etched with 37% phosphoric acid gel, rinsed and dried. Adhesive resin of SBMP was applied over amalgam and tooth margins and polymerized (rebonding). Specimens were thermocycled, exposed to dye and sectioned. Microleakage was graded (0-3) using a stereomicroscope at X40 magnification. Data were analyzed with Kruskal-Wallis, Mann-Whitney and Wilcoxon pair wise statistical tests. P<0.05 was considered as the limit of significance.

Results: The groups lined with SBMP showed the lowest and the groups without liner the highest microleakage (p= 0.001). Significant difference was observed in microleakage mean rank of enamel and dentin margins (p=0.048). Rebonding with resin did not improve the seal (p> 0.05).

Conclusion: Based on the results of this study, total etch adhesive system had significant effect on microleakage of Class V amalgam restorations especially in cervical margin. Rebonding did not show a significant effect on microleakage.

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Background and Aim: Amalgam is one of the mostly used restorative materials, but has some disadvantages. Microleakage is one of the short comings of amalgam which may lead to sensitivity and recurrent caries. The aim of this study was to evaluate the effect of three dentin bonding systems on reduction of microleakage in amalgam restorations.

Materials and Methods: Class II amalgam restorations were made in 40 noncarious molar and premolar teeth. Then the specimens were divided into four equal groups. Scotch Bond Multi Purpose, Single bond,
iBond, were used as liner in groups one to three respectively and in group four no liner was used. The teeth were restored with high copper spherical amalgam. After thermocycling for 500 cycles at 5⁰C and 55⁰C, the specimens were immersed in basic fuchsin for 24 hours, bisectioned mesiodistally and evaluated under stereomicroscope at X25 for dye penetration. The data were analyzed by Kruskal-wallis and Scheffe. P<0.05 was considered as the level of significance.

Results: The groups showed significant difference (p=0.003). The group four had significantly less microleakage than the first and second groups (p<0.05). The second and third groups showed significantly different microleakage (p=0.038).

Conclusion: Based on the results of this investigation applying dentin bonding agents has no effect on reducing microleakage in amalgam restorations, however more studies are recommended.

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Background and Aim: Fractured anterior teeth can be restored by adhesive bonding of the separated fragment to the remaining tooth structure. This invitro study evaluated the force required for fracture of rebonded fragments which were dried and rewetted for various time intervals prior to bonding.

Materials and Methods: One hundred and eight human mandibular incisors were selected and were divided into 9 groups and then were fractured. The fragments were stored in air at room temperature and ambient humidity. They were air dried for 30 minutes in group 1, 2 and 3,  6 h in group 4 and 5,  24 h in group 6 and 7, and 3 days in groups 8 and 9. The apical parts of the fractured teeth were stored in water.After storage in air, each fragment in group 1 was bonded to the remaining apical tooth structure with a bonding agent and flowable composite.In groups 2, 4, 6 and 8 after air drying the fragments were stored in water for 30min. The time of storage in water in groups 3, 5, 7, 9 was 24 hours. Then each fragment was bonded to the apical part structure. The mean force required for fracture was measured by a mechanical testing machine. One way ANOVA and Tuckey tests were used for analysis.

Results: There were statistically significant differences between different groups (p<0.001).The highest and lowest force required for fracture belonged to groups 3 and 8 respectively. The force required for fracture in group 1 was lower than group 2, 3, 5 and 7 and 9 and more than groups 4,6 and 8 with significant differences.While there were no significant differences among groups 3, 5, 7, 9 and 4, 6, 8 the force required for fracture in groups 2, 3, 5, 7 and 9 was more than 4, 6, 8 with significant differences.

Conclusion: This research revealed that the force required for fracture was affected by air storage of fragments prior to bonding. Also showed that with an increase in duration of drying the force required for fracture decreased and after a period of time it becomes steady. This study showed that in order to increase the fracture strength after air drying the fragment should be immersed in water for at least 24 hours prior to bonding.

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Background and Aim: As composite-dentin bond strength is affected by cavity preparation and the bond strength of composite resin to new and used bur prepared dentin has not yet been evaluated, this study evaluated the effects of cutting dentin with different cutting efficacy (new and used) of burs on composite-dentin shear bond strength using self-etching primer bonding system and total etching bonding system.

Materials and Methods: Sixty caries free human 3rd molar were sectioned in occlosal surface to expose dentin, then polished with silicon carbide paper and randomly divided into four groups. Each group was prepared in a depth of 0.5mm of dentin, using new diamond bur, or used diamond bur. To change into a used bur, each new rough diamond bur had to work on bovine enamel for 30 minutes, under a load of 150g. Then, each group was bonded, using a total etch adhesive (single Bond) or a self etch adhesive (clearfil SE Bond) So there were 4 groups : 1-SE Bond, New bur 2-SE Bond , used bur 3-Single Bond , New bur 4-Single Bond, used bur. Similar composite capsules(Filtek Z250) were bonded to dentin surface and cured. specimens were stored in physiologic saline for 48h at 37⁰ c , then put under shearing load to define composite - dentin shear bond strength. Results were interpreted via statistical analysis (T-test & two - way variance).

Results: Shear bond strength of each group was as follows: 1-(27.3Mpa), 2-(33.5Mpa), 3-(16.9Mpa) 4-(19.3Mpa). Statistical analysis proved that shear bond strength of used diamond bur prepared groups (2,4) was more than new diamond bur prepared ones (1,3). This statistical difference, specially, was seen between SE Bond groups (1,2) but not between single Bond groups (3,4). Also, shear bond strength of (SE Bond) bonded groups (1,2) were more significantly than (single Bond) bonded ones (3,4).

Conclusion: This study show that Bur cutting efficiency influences composite - dentin shear bond strength especially when the adhesive is SE Bond (used bur> new bur) Also type of adhesive affects on composite - dentin shear bond strength. (SE Bond > Single Bond).

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Background and Aim: At the end of fixed orthodontic treatment, enamel damages are affected by amount of adhesive remnants. Thus, determining the factors involves in the amount of adhesive remnants e.g. the types of resins and the methods used for debonding is of necessity. The aim of this study was to evaluate the effect of the composite resin type and debonding pliers on enamel surface by a new method in measuring the adhesive remnant.

Materials and Methods: In this interventionally in-vitro study, 120 extracted human premolars were randomly divided into four groups of 30 each. The standard edgewise metal brackets were bonded on the teeth with two pastes self-cure composite "Concise" in the first and second groups, and with No-mix composite "Unite" in the third and fourth groups. the debonding was done by means of Lift off Debonding Instrument "LODI" (3M-Unitek), in first and third groups, and Bracket Removing Plier (Dentaurum), in the second and forth groups, respectively. After debonding, all teeth were evaluated with steriology software (stereolith version 1) for comparing the amount of adhesive remnants between the four groups. For data analysis, One-way ANOVA and independent t.test were used.

Results: The fourth group showed the lowest adhesive on the enamel surface and the most on the bracket surface with significant differences with other three groups (p<0.01).

Conclusion: The use of Dentaurum debonding plier with shear-peel force when used with the Unite adhesive (group 4), would decrease the adhesive remnants on the enamel surface.

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Background and Aim: Extent of polymerization (Ep) is the rate at which methacrylate C=C bonds are converted to aliphatic C-C bonds. The higher the Ep value, the better the polymerization of the polymer. If the polymer dose not polymerize sufficiently, it could have destructive effects e.g. releasing monomer, initiator, free radical or insufficient polymerization of hybrid layer. Therefore, measuring the Ep value is very important. Light-emitting diodes (LEDs) are becoming increasingly popular in dental practice as they have a long life expectancy. LEDs do not generate infrared wavelength, and have a constant light output. Therefore, comparison of LED and Quartz tungsten halogen (QTH) from the point of Ep is important. Differential Scanning Calorimetry (DSC) is a proper tool for determining the Ep value, monitoring the process of reaction and reaction kinetics. The aim of this study was to measure the Ep value of a dental bonding containing camphorquinone/amine photoinitiator which were cured by LED and QTH using DSC.

Materials and Methods: In this experimental study, 2.5 mg of bonding material were placed in DSC aluminum pans. Two different light cures, LED & QTH (Coltene Company Coltolux ®75 LED Curing Light), were used in this study. The light guide was positioned at a distance of 9mm from the base of the sample chamber. Each sample was photopolymerized for 30 seconds, and the DSC curves were obtained after 100 seconds from initiation of photopolimerization of each sample, at both 23^oC and 37^oC. The heat of photopolymerization (DH) was calculated from the area under the peak of the differential temperature curve. Five samples were used for each condition. The data was analyzed by two-way ANOVA.

Results: There was highly significant difference between two temperatures in the experimental bonding (pvalue<0.001). Representative DSC curves showed the same kinetic behavior for LED and QTH.

Conclusion: The results revealed that the Ep generated by high power density QTH was the same as that of produced by second generation of LED.

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Background and Aim: Since the clinical studies conducted to explore the effect of bonding agents on the success of fissure sealant are limited in number, the present study was conducted to compare the effects of two bonding agents on the success of fissure sealant of the saliva- contaminated teeth.

Materials and Methods: In this study, 63 teeth of 35 students aged 6-8 with healthy and newly-erupted upper jaw molar teeth, were selected and divided into two groups. In one group, single-bond and In the other, SE bond were used. As the teeth were newly erupted and the distopalatal area being in contact with the gingival sulcus and this fact that, this area was impossible to isolate, the distal sulcus of the contaminated teeth and the mesial area capable of adequate isolation were assumed isolated. For each group, after etching, the teeth on one side were treated with a sealant and the teeth on the other side were first bonded and then treated with the sealant. Hence, four groups were formed: bonded/isolated, bonded/contaminated, controlled/isolated and controlled/contaminated for any bonding agent. The results of the treatment were assessed in terms of the success and failure and then analyzed using Mc Nemar, and Chi-square tests and Cochran.

Results: The results of the study indicated that:1. Saliva contamination caused a significant increase in sealant failures(p<0.05).2. Use of the two bonding agents significantly increased the success of the sealant(p<0.05).3. Use of the two bonding agents significantly increased the success of the fissure sealant on the saliva-contaminated teeth, even more than when the teeth had been isolated and with only fissure sealant(p<0.05). 4. For the contaminated enamel, the results of SE bond were better than those of the single bond (p<0.05). However, in isolated condition the results of using single bond were better (p<0.05).

Conclusion: The results of the study supported the use of these two bonding agents in the treatment of fissure sealants under both isolated and contaminated conditions. Further, more SE bond seemed to be less sensitive to saliva contamination.

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Background and Aim: Evaluation of shear bond strength and microleakage of bonding agents is important as these properties play main roles in adhesion of composite to dental tissues. Microleakage results in bacterial penetration into dentin tubules and enamel surfaces and causes sensitivity and recurrent caries followed by destruction of composite filling. Insufficient shear bond strength results in early failure of filling in low masticatory forces. The main goal of this study was to compare the microleakage and shear bond strength of an experimental adhesive and Scotchbond multi-purpose (MP) adhesive system.
Materials and Methods: In this experimental study, sixty extracted caries free human molar teeth were randomly assigned into 4 groups of 15 each for shear bond strength. Variables were bonding agents, enamel and dentin. Twenty teeth assigned into 2 groups of 10 each were used for valuation of the microleakage. Microleakage and shear bond strength were performed according to ISO TR 11405. All data were analyzed with parametric and non-parametric tests according to their normality distribution. Also, Weibull distribution performed on data.
Results: Data obtained from both microleakage and shear bond strength tests showed no significant difference between the experimental bonding and Scotchbond MP bonding (P>0.05). Furthermore, there was no significant difference between the microleakage of occlusal and gingival parts of both bondings (P>0.05).
Conclusion: Experimental adhesive bonding showed acceptable results regarding microleakage and shear bond strength. It may be concluded that the experimental dentin bonding had a comparable performance quality with that of commercial system.

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Background and Aims: In this in-vitro study, the effect of multiple adhesive coating on the microshear bond strength of composite to dentin and surface microhardness of dentin after treatment with four adhesives (One Step Plus, One Step, Single Bond, Single Bond 2) were evaluated.

Materials and Methods: One hundred intact human molars were cut to obtain disks of dentin having 2 mm thickness. For the microshear bond test, sixty disks were randomly divided into four groups. In each group one type of adhesive was used. In one half of a disk two layers and in another half six layers of adhesive were applied. Cylinders with 1mm height was filled with a composite and light cured. The cross-head speed was 0.5 mm/min. Vickers microhardness was tested on forty dentin disks which divided into four groups and prepared in the same manner used for microshear bond test. Data were analyzed by Two-way ANOVA and Tukey tests.

Results: The highest and lowest bond strength were recorded as 29.49 ± 5.74 MPa (One Step Plus 6 layers), and 21.23 ± 4.83 MPa (One Step Plus 2 layers), respectively. The results indicated that One Step Plus bond strength in 6 Layers was significantly higher than 2 layers. The highest and lowest dentin hardness values were
39.08 ± 8.34VHN (Single Bond 2 layers) and 28.53 ± 5.98 VHN (One Step Plus 6 layers). None of the adhesives exhibited significant difference in hardness with regards to the layers applied (P>0.05). Presence of filler in adhesives had no significant effect on bond strength (P=0.05) whereas caused significant decrease in the dentin microhardness (P<0.05). In addition, type of solvent had significant effect on the bond strength and bond strength was significantly higher in acetone-base adhesives (P<0.05). However, dentin microhardness was significantly higher in the ethanol-base adhesives (P<0.05).

Conclusion: Multiple adhesive coating had no influence on the microshear bond strength of composite to dentin and dentin surface microhardness. It was dependent on the type of adhesive used.

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Background and Aims: This ex vivo study was done to evaluate the effect of different adhesive systems on microleakage of class V composite restorations.
Materials and Methods: Thirty extracted human third molar teeth were selected and class V cavities were prepared (3×3×1.5 mm) on buccal and lingual surfaces. Then, teeth were divided into 3 groups. Adhesives used in this study were Excite (Ivoclar/Vivadent), AdheSE(Ivoclar/Vivadent) and AdheSE-one Ivoclar (Vivadent/Vivadent). After application of adhesives, cavities were restored with a resine composite (InTen-S A1/ Ivoclar), in 3 increments and cured with QTH light curing unit (700 mW/cm). After 24 hours storage of teeth in 370C water, teeth were thermocycled (500 cycles in 5-550C) and stored in 1% basic fushin for 24 hours. After that, the specimens were rinsed with running water and mounted in a self-cured acryl. Finally, the specimens were sectioned and maximum depth of dye penetration for each restoration was measured using stereomicroscopy. The results were statistically analyzed with Mann-Whitney U test.
Results: In comparison between enamel and dentin margins in each group, microleakage in enamel margins were less than that of dentin margins. This difference was statistically significant in Excite and AdheSE-one groups (P=0.001, P=0.043). AdheSE showed the least microleakage in dentin margins however, there was no significant difference between 3 bonding agents (P=0.14). In enamel margins, there were significant differences between 3 bonding agents (P=0.001). Excite showed the lowest microleakage and AdheSE-one had the highest microleakage.
Conclusion: The least enamel microleakage was associated with the total-etch and least dentin microleakage was observed in the two-step self-etch adhesive system.

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Background and Aims: The aim of this in vitro study was to assess the shear bond strength of amalgam to dentin using four dentin adhesive systems.
Materials and Methods: One hundred human molars were selected. After enamel removal, a dentin cylinder with 3 mm thickness was prepared. Eighty specimens were resorted with amalgam and four dentin adhesive systems as follows (n=20): group 1, Scotch Bond Multi-Purpose group 2, One Coat Bond group 3, PQ1 and group 4, Panavia-F. In group 5, 20 specimens were resorted with amalgam and varnish as control group. The specimens were incubated at 37°C for 24 h. The shear bond strengths were then measured by using push out method. The data were analyzed by one-way ANOVA and post hoc Duncan's tests.
Results: Mean values for bond strengths of test groups were as follows: group 1=21.03±8.9, group 2=23.47±9, group 3=13.16±8.8, group 4=20.07±8.9 and group 5=14.15±8.7 MPa±SD. One-way ANOVA showed the statistically significant difference between the bond strengths of five groups (P=0.001). Post hoc Duncan's test showed significant difference between groups 1and 3 (P=0.008), groups 1 and 5 (P=0.019), groups 2 and 5 (P=0.0008), groups 4 and 5 (P=0.042), and groups 3 and 4 (P=0.018).
Conclusion: Results of this study showed that the bond strength of amalgam to dentin using One Coat Bond as dentin adhesive system was higher than that observed in other dentin adhesive systems.