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Showing 4 results for Bone Regeneration
Histologic analysis of osteopromotion property of homogenous demineralized dentin Matrix in parietal bone defects in rabbit
Hr. Azimi, N. Bakhshalian, H. Shahoon,
Volume 22, Issue 2 (11-2009)
Abstract

Background and Aim: The aim of this investigation was to evaluate the osteopromotion property of homogenous demineralized dentin matrix (HDDM) on experimental surgical bone defects in parietal bone of rabbits using the guided bone regeneration (G.B.R.) technique incorporating Paroguide collagen membrane.

Materials and Methods: Surgical bone defects were created in 6 Newzland white rabbits (2 defects in each rabbit). The defects were protected by Paroguide membrane alone (control group) or filled with HDDM and protected by Paroguide membrane (experimental group). The HDDM had been obtained from the central incisors of rabbits. The rabbits were sacrificed after 15, 30, 45, 60, 75 and 90 days and the defects examined histologically. Data were analyzed using pair-t test. The level of significance was set at p=0.03.

Results: Histologically, the volume of newly formed bone matrix was significantly greater in the experimental group. No inflammatory reaction was seen in either experimental or control groups.

Conclusion: Bone regeneration was accelerated in the bone defects filled with HDDM in comparison to the control group.


Histologic and histomorphometric study on the effects of lactoferrin and porous bovine bone mineral (Bio-Oss) on the regeneration of bone defects made on rabbit calvarium
M. Paknejad, A. Rokn, A. Sabur, F. Elhami,
Volume 23, Issue 3 (12-2010)
Abstract

Background and Aims: Nowadays reconstruction of alveolar defects has become one of dentists' problems especially in areas which are going to get dental implants. Inorganic bovine bone mineral (Bio-Oss) is one of the most popular graft materials that acts as a structure for migration of osteoblasts. If migration, proliferation, and differentiation of osteoblasts can be promoted by a material, it would be possible to reconstruct more amount of bone in a shorter period of time. Milk contains vital proteins that regulate bone growth. One of these important proteins is lactoferrin. The aim of this study was to examine the effect of added bovine lactoferrin to Bio-Oss on osteogenesis.
Materials and Methods: Two doses of 50 and 500 µg/ml of lactoferrin were prepared. Ten New Zealand white rabbits were selected for this study. Four 6-mm symmetrical detects were created in each rabbit's calvarium. Two of these sites were filled with Bio-Oss that was wetted with two doses of lactoferrin. Third detect was filled with Bio-Oss alone and the forth one was left empty as control group. After 4 weeks histologic and histomorphometric analysis was performed.
Result: There was no sign of obvious inflammation in any of four groups. Also there was no difference among four groups in terms of vitality, type of new bone, and foreign body reaction. However, amount of bone formation in control group was significantly lower compared with the other 3 groups. Although lactoferrin containing groups showed little increase in bone formation especially in higher concentration, there was not statistically significant difference among the three test groups. Amount of remaining biomaterial also was lower in lactoferrin containing groups compared with the Bio-Oss group but the differences were not significant.
Conclusion: Although there was no significant difference among the test groups, it seems that the added lactoferrin increases bone formation. Considering the limitations of this study, more studies are needed in different concentrations of lactoferrin and different healing periods. Furthermore, because of possible washout of the lactoferrin from the defects, it would be helpful to find and evaluate a proper carrier agent for lactoferrin to see its real effects.


The role of barrier membranes in guided bone regeneration; A review
Farzane Jabari, Behzad Houshmand, Saeed Hesaraki,
Volume 31, Issue 3 (11-2018)
Abstract
The Guided Bone Regeneration (GBR) treatment concept advocates that regeneration of osseous defects is predictably attainable via the application of occlusive membranes, which mechanically exclude non-osteogenic cell populations from the surrounding soft tissues, thereby allowing osteogenic cell populations originating from the parent bone to inhabit the osseous wound. The use of membrane to exclude non-osteogenic cells, is a key principle of guided bone regeneration. A large number of membranes have been evaluated in clinical and experimental studies. The object of this study was to review the literature regarding guided bone regeneration and all types of membranes that were used in this technique. 72 articles between the years 1968 through the 2016 from PubMed, Medline and Google Scholar using the related keywords, were selected. Finally, we concluded that the modification of mechanical and physico-chemical properties of membranes could improve the process of new bone growth. However, determination of the exact role of membrane porosity in this process, still needs to be clarified. Optimization the chemical composition of membrane with the focus and attention to obstructive property and bioactivity, is an important point in this research field. Various factors such as flexibility, mechanical strength and degradation rate determine the type of membrane used for bone tissue regeneration.

The effect of freeze-dried bone allograft and partially demineralized freeze-dried bone allograft on regeneration of rabbit calvarial bone defects: A Histological and histomorphometric study
Mohammad Najafi, Hassan Semyari, Rokhsareh Sadeghi, Fatemeh Mashhadiabbas, Fereshteh Shanei,
Volume 32, Issue 3 (11-2019)
Abstract
Background and Aims: Reconstruction of osseous defects is one of the ideal goals of periodontal treatments and dental implant therapy. Different biomaterials have been used for this purpose and many studies have tried to compare and introduce the best ones. The present study aimed to evaluate the effect of PDFDB (Partially Demineralized Freeze-Dried Bone Graft) and FDBA (Freeze Dried Bone Allograft) on the regeneration of rabbit calvarial defects.
Materials and Methods: In this experimental study, 48 similar defects with the diameter of 8 mm were generated in the calvarium of 16 rabbits. Two defects were filled with FDBA and PDFDB, while the other one remained unfilled as the control group. All defects were covered by collagen membranes. 6 and 12 weeks after surgery, the histologic and histomorphometric tests were performed to evaluate the following variables: the rate of new bone formation and its type, the amount of residual grafting material, degree of inflammation and pattern of bone formation. Comparison of osteogenesis percentage and residual biomaterial was performed by repetitive variance analysis, whereas qualitative variables were compared by Friedman non-parametric test.
Results: Regarding bone formation percentage, there was no statistically significant difference between three different groups at 6-week (P=0.33) and also at 12-week time points (P=0.98). The amount of residual material in the PDFDB group was significantly lower than FDBA (P=0.04) in the 6-week samples. However, this difference was not significant in the 12-week samples (P=0.41). Bone quality showed statistically significant difference between graft containing groups and control group after 12 weeks (P=0.01). It means, all samples in FDBA and PDFDB groups displayed lamellar bone after 12 weeks while in control group, only woven bone or a combination of woven and lamellar bone was seen.
Conclusion: FDBA and PDFDB demonstrated similar regenerating effect in the rabbit calvarial bone defects and hastened bone maturation compared to the non-grafted defects.

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