Journal of Dental Medicine

Statement of Problem:The surface topography of denture base material is an important factor for the adhesion of Candida albicans and other microorganisms.

Purpose: The aim of this study was to evaluate the adherence of Candida albicans to four types of denture base materials (Acropars acrylic resin, Meliodent acrylic resin, rough and smooth surfaces of Molloplast B).
Materials and Methods: Seven blocks of two types of acrylic resins and ten blocks of silicone with one rough and one smooth surface were made and incubated in a suspension of Candida albicans. After washing,the blocks were stained with acridine orange and examined under fluorescent microscope. For statistical analysis ANOVA and Duncan tests were used.

Results: It was observed that Candida adhesion to rough surfaces of acrylic resins and silicone was significantly more than polished surfaces of acrylic resins and smooth silicone (PO.0001). However, no
statistical significant difference was found between polished acrylic resins surfaces and smooth silicone.
Conclusion: Significant differences in the adherence of Candida to the surfaces of different denture base
materials are due to differences in surface topography, chemical, physical and hydrophobic properties so it is
recommended to minimize the roughness and irregularities of denture base.

Background and Aims: The aim of this study was to investigate the effects of sodium bicarbonate against candida albicans, the main infectious factor of denture stomatitis, and to compare it with other common anti-candida medications.

Materials and Methods: Three concentrations of Candida albicans (105, 106, 107 CFU/mL) were prepared. Experimental substances were 5% sodium bicarbonate, nystatin, chlorhexidine, and sterile saline (as control). Each of them was added separately to the same amounts of Candida albicans of different concentrations, and sampled at the intervals of 1, 2, 3 and 4 min. Then, the samples were cultured and incubated. The number of formed colonies was counted for each plate. The data were analyzed, using ANOVA and Tukey test.

Results: Sodium bicarbonate showed a noticeable anti-candida effect on the concentrations of 105 and 106 CFU/mL, with almost similar anti-candida effect as nystatin and chlorehexidine(P=0.29, P=0.32). Nystatin was the most effective medication on the concentration of 107 CFU/mL of candida (P=0.03, P=0.01). Sodium bicarbonate and chlorhexidine were less effective in this concentration with no statistically significant difference (P=1.00).

Conclusion: Sodium bicarbonate showed a significant anti-candida effect at low and medium concentrations of Candida albicans, but nystatin was more effective in the higher concentrations.