S. Arami , M. Tavassoti Kheiri , M. Hasani Tabayabaie , E. Yasini , A. Pahlavan , M. Ghavam , M. Mirzaie , H. Kermanshah , Sh. Forootan , S. Ahrabi , M. Tabatabaian , L. Mahrokh ,
Volume 20, Issue 4 (1-2008)
Abstract
Background and Aim: The efficiency of disinfecting materials and procedures in removal of contamination from dental surfaces and equipments is essential. In authors' previous study, daily use of 2:100 dilution of 5% sodium hypochlorite in water and disposable covers were recommended since HBV contamination was found on semi-critical parts of the operative dentistry department. The aim of this study was to evaluate the HBV contamination following application of the recommended procedures.
Materials and Methods: The study was conducted in two parts. In the first cross-sectional part, samples were collected from 17 sites of dental surfaces. In the second interventional part samples were collected from 10 sites of 9 dental and 3 sites of 2 light cure units, before and after disinfection with 5% sodium hypochlorite solution diluted to 2:100. Sterile cotton swabs moistened with sterile BSAS (Bovine Serum Albumin in Sodium Chloride) solution were used for sampling. Samples were tested by PCR technique in Pasteur Institute, Iran.
Results: None of the samples collected in the first part of the study showed contamination. In the second part of the study, from 96 samples taken from various parts of dental and light cure units, before and after disinfection, there was only one HBV contaminated site before disinfection which showed no contamination after disinfection.
Conclusion: Based on the results of this study, disinfecting procedure with 5% sodium hypochlorite solution diluted to 2:100 along with using disposable covers is effective in preventing HBV contamination.
M. Tabrizizadeh, Sm. Abrisham, F. Dehghan Marvasti,
Volume 22, Issue 3 (12-2009)
Abstract
Background and Aim: Extracted teeth used in preclinic should be disinfected. The aim of this study was to evaluate the effects of some disinfectants on microorganisms cultured from pulp chamber of extracted teeth.
Materials and Methods: In this experimental study 54 intact human teeth were collected. After access cavity preparation, 10 8 B. streothermophillus endospors were inoculated into pulp chamber. Then cavities were sealed with a temporary restorative material. Teeth were divided into 5 groups of 10 each. The teeth were then stored in these disinfectants: 5.25% hypochlorite sodium, 5% Microten, 5% Deconex, 2% Glutaraldehyd, and 10% Formalin for 48 hours. Two teeth were autoclaved as negative controls and two were stored in normal saline as positive controls. The teeth were then sectioned in cervical area and cultured in Trypticase Soy Broth. After three days turbidity in tubes was evaluated. Statistical analysis was done by Fisher's exact test.
Results: None of these solutions were able to prevent microorganism growth in all samples however, Formalin was better in six cases than that of other disinfectants. Differences between these five groups were not statistically significant (P=0.384).
Conclusion: Sterilization of the teeth with autoclave is the only absolute method for disinfecting the root canals of extracted teeth and disinfectants are not reliable for this purpose.
Arami Sakineh , Aghasadeghi Mohamadreza , Tabatabaie Masome Hasani , Darvishpour Hojat , Sheikhzadeh Sedigheh ,
Volume 27, Issue 4 (1-2015)
Abstract
Background and Aims: Hepatitis B is an important infection route in dentistry requiring different disinfectants to prevent its transmission. The aim of this study was to compare the effects of chemical disinfectants (FD366, ISORAPID and 5% sodium hypochlorite 2/100) to remove Hepatitis B infections from the dental surfaces.
Materials and Methods: In this experimental laboratory trial, serum of 10 HBV patients was poured into microtubes, FD366, ISORAPID and hypochlorite disinfectants were added to them. PCR experiments with viral diagnostic kits were used to diagnose the virus genome. Real time PCR was used to evaluate after incubation with the disinfectants. The reductions occurred in the viral load of Hepatitis B were statistically analyzed using Kruskal-wallis and Mann-Whitney U tests .
Results: No significant antiviral efficacy was noted following the application of FD366 and ISORAPID disinfectants (P=0/07). However, hypochlorite showed the most efficacy to disinfect Hepatitis B and a significant difference was found among them (P<0.0001).
Conclusion: Under the study limitations, FD366 and ISORAPID disinfectants did not show adequate efficacy to remove Hepatitis B virus. Hypochlorite was the most effective disinfectant.
Zohreh Moradi, Masumeh Hasani Tabatabaei, Abolfazl Fateh, Mohammad Javad Torkamani, Ladan Ranjbar Omrani, Sara Valizadeh,
Volume 31, Issue 4 (1-2019)
Abstract
Background and Aims: Methicillin-resistant Staphylococcus Aureus (MRSA) is an important infection source in dentistry for different disinfectants to be used to prevent its transmission. Furthermore, a variety of chemical disinfectants are developed to remove bacterial infections from the dental care workers' hands and claims are made regarding their superiority in infection control, although all requires scientific investigations. The aim of present study was to compare the effects of chemical hand-disinfectants Micro Zed HD, Aseptoman and Decosept on MRSA (methicillin resistant staphylococcus aureus) in different periods of time.
Materials and Methods: In this experimental study, the antibacterial efficacy of three hand-disinfectants on standard strain of MRSA (USA300: ATCC® BAA-1717™) was evaluated according to European standard of evaluating antiseptics (EN 1040:2005 CSN EN). First we prepared a 0.5 Mc Farland (108 CFU/ml) suspension of MRSA, and exposed to three disinfectants for 15, 30, 60 and 90 seconds. Then, they were transferred to separate plates of Mueller-Hinton medium and incubated in 37◦C for 24 hours. The plates were compared then with control plate to evaluate the efficacy of materials on bacteria by calculating the CFU/ml of plates.
Results: Three hand-disinfectants evaluated in this study had the maximum anticabterial effect on MRSA in the minimum time of exposure (15 seconds), and we found no trace of growth in any plates.
Conclusion: The three hand-disinfectans (MicroZed HD, Aseptoman and Decosept) in the concentration suggested by their companies, showed no difference in efficacy to remove MRSA from hands.
