S. Arami , M. Tavassoty Kheiri , R. Bashar , Ma. Nadali ,
Volume 19, Issue 1 (4-2006)
Abstract
Background and Aim: Infection control is an important issue in dentistry. Without an efficient infection control, pathogens left on instruments and working surfaces will have potential danger to patients’ health. In this research, antiviral effect of three disinfectants: 0.5% sodium hypochlorite 0.05% sodium hypochlorite and Deconex 50 AF, on HBV was investigated.
Materials and Methods: In this interventional (before-after) study serums of 26 HBV positive patients were analyzed by PCR HBV analysis and 9 contaminated species were obtained to test three disinfectants. 36 agar plates were prepared with the contaminated serums. 27 of the plates were disinfected in 3 separate groups with the above mentioned solutions. Nine remaining plates were not disinfected (control). Swabs wetted by BSAS (Bovine Serum Albumin Sodium Chloride) medium were applied on the surface of the plates and the were kept in the transferred medium and sent to virology-lab of Pasteur Institute. HBV DNA were detected by commercial kit of HBV PCR (polymerase chain reaction) method. Data were analyzed by Cochrane test with p<0.05 as the limit of significance.
Results: None of samples disinfected with 0.5% sodium hypochlorite showed contamination. 11/1% of samples disinfected with 0.05% sodium hypochlorite and 44/4% of samples disinfected with Deconex 50 AF remained contaminated. Statistical analysis showed a significant difference between 0.5% sodium hypochlorite and the other groups.
Conclusion: Our findings revealed that 0.5% sodium hypochlorite solution is a strong and efficient disinfectant against HBV. Key Words: HBV Sodium hypochlorite
S. Arami , M. Tavassoti Kheiri , M. Hasani Tabayabaie , E. Yasini , A. Pahlavan , M. Ghavam , M. Mirzaie , H. Kermanshah , Sh. Forootan , S. Ahrabi , M. Tabatabaian , L. Mahrokh ,
Volume 20, Issue 4 (1-2008)
Abstract
Background and Aim: The efficiency of disinfecting materials and procedures in removal of contamination from dental surfaces and equipments is essential. In authors' previous study, daily use of 2:100 dilution of 5% sodium hypochlorite in water and disposable covers were recommended since HBV contamination was found on semi-critical parts of the operative dentistry department. The aim of this study was to evaluate the HBV contamination following application of the recommended procedures.
Materials and Methods: The study was conducted in two parts. In the first cross-sectional part, samples were collected from 17 sites of dental surfaces. In the second interventional part samples were collected from 10 sites of 9 dental and 3 sites of 2 light cure units, before and after disinfection with 5% sodium hypochlorite solution diluted to 2:100. Sterile cotton swabs moistened with sterile BSAS (Bovine Serum Albumin in Sodium Chloride) solution were used for sampling. Samples were tested by PCR technique in Pasteur Institute, Iran.
Results: None of the samples collected in the first part of the study showed contamination. In the second part of the study, from 96 samples taken from various parts of dental and light cure units, before and after disinfection, there was only one HBV contaminated site before disinfection which showed no contamination after disinfection.
Conclusion: Based on the results of this study, disinfecting procedure with 5% sodium hypochlorite solution diluted to 2:100 along with using disposable covers is effective in preventing HBV contamination.
