Journal of Dental Medicine

Statement of Problem: Epulides Fissurata (EF) are common proliferative and denture- induced lesions of the oral cavity with a predilection for female. This suggests a possible role for sex steroid hormones in the development and progression of these lesions.

Purpose: The objective of this study was the immunohistochemical evaluation of epulis fissuratum of the oral cavity for estrogen and progesterone receptors expression in epithelial, stromal, inflammatory and endothelial cells populations.

Materials and Methods: In this cross-sectional study, 15 samples of formalin- fixed, paraffin- embedded epulis fissuratums including marginal mucosal tissues in 4 cases as a control group, were immuno-histochemically evaluated for estrogen and progesterone receptors protein expression.

Result: In 10 cases, estrogen receptor positivity was found within the epithelium and progesterone receptor immunoreactivity was present in 7 cases. Stromal cells exhibited estrogen and progesterone receptor immunostaining in many cases but only few cases showed expression of these receptors in the inflammatory and endothelial cells. Estrogen and progesterone receptors were also detected in some cases containing salivary glands tissue.

Conclusion: Although chronic irritation may be the initiating factor for the occurence of epulis fissuratum, some of the cells in the lesion, could be potential targets for estrogen and progestrone hormones.

Background and Aim: Giant cell granuloma is a relatively common benign proliferative lesion of the oral cavity. This lesion has a marked gender predilection with more prevalence in females and tendency to rapid growth and recurrence during pregnancy. The aim of this study was the evaluation of specific receptors of sex hormones in giant cell granuloma.

Materials and Methods: In this cross-sectional study, twenty five cases of formalin fixed paraffin embedded giant cell granulomas were retrieved from the oral pathology archive of dental school Isfahan University of Medical Sciences. Also twenty five normal oral mucosa biopsies resected during different surgical procedures were prepared as control group. Cases were immunohistochemically stained for estrogen and progesterone receptors using the biotin-streptavidine method. Data were analyzed by SPSS package.

Results: Staining for ER/PR markers were negative for the mononuclear stromal cells and multinucleated giant cells in all cases. The epithelial cells and connective tissue stromal cells of the control group were also negative for these receptors.

Conclusion: Based on the results of this study, immunostaining for ER/PR was negative in all cases. These findings suggest that in most cases development and growth of this lesion is not directly related to these hormones. However further studies with more sensitive techniques are recommended.

Background and Aim: Angiogenesis plays a central role for development and progression of malignant tumors.It is considered as an important factor for predicting of malignant tumor's behavior such as metastasis to lymph nodes and other clinicopathologic factors. However , it is still a controversial factor especially in oral squamous cell carcinoma.The aim of this study was to evaluate the correlation between angiogenesis and clinicopathologic parameters such as presence of metastatic cervical lymph node in the tongue squamous cell carcinoma.

Materials and Methods: In this cross-sectional study, 40 cases of squamous cell carcinoma of the tongue were selected from the archive of cancer institute of Tehran University of Medical Science. Sections were prepared from paraffin blocks and immunohistochemically stained with antibody against CD34. Stained vessels were counted in 4 fields ,the most vascular areas at low magnification, in each areas of intratumoral ,peritumoral and nontumoral adjacent tissue in two groups with metastatic lymphnodes (N+) and without (N-). The average counts from the four most vascular areas were recorded as the mean microvascular density (MVD). Data were analyzed by 3wayANOVA and Independent T- test with p<0.05 as the level of significance.

Results: High mean MVD-CD34 was significantly correlate with positive cervical lymph node metastasis in intra tumoral and peritumoral areas but there was no significant correlation between mean MVD-CD34 and age, gender, and differentiation of tumor.

Conclusion: Based on the results of this study, CD34 can help us to determine the presence of cervical lymph node metastasis and may also determine the outcome of a primary squamous cell carcinoma of the tongue.