Journal of Dental Medicine

Statement of Problem: Elimination of porphyromonas gingivalis (p.g) from subgingival area in order to successfully treatment out comes in patients with Aggressive periodntitis AP is necessary.

Purpose: The aim of this study was the evaluation of non-surgical treatment efficacy in reduction of bacterial population in deep pockets.

Materials and Methods: In this randomized clinical trial study we evaluated the result of non- surgical therapy on reduction of p.g count from deep pockets of patients with aggressive periodontitis that had at least one (p.g plus) deep pocket (>5mm) in each quadrant. At first stage of non-surgical treatment intra pocket irrigation with chlorhexidin was done after scaling and root planning for all patients. In second stage (one week later) antibiotics including amoxicillin- metronidazol prescribed for ten days. At base line, one, six and twelve weeks after beginning of therapy, microbial samples, plaque index, bleeding on probing index and probing pocket index were recorded.

Result: There was statistically important difference between one and six weeks after treatment with base line in colony count of p.g and all of clinical indices. But in 12 weeks after therapy just, PI and PPD had statistical difference with base line. In this stage, colony count and BOP was reduced but this reduction had not statistically important difference with base line.

Conclusion: Thus in present study our non- surgical strategy in elimination of p.g and clinical improvement was successful in short time but three month after therapy recurrence of disease happened in some patients.

Statement of Problem: One of the best ways for treatment of Aggressive Periodontitis (AP) is identification and elimination of etiologic factors specially two microorganisms Actinobacillus actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg) in patients harboring them.

Purpose: This study determines the prevalence of Aa and Pg and its correlation with age, sex and the number of family members as well as probing pocket depth (PPD) in active sites of AP patients, referred to department of periodontics, Faculty of Dentistry, Tehran University of Medical Sciences.

Materials and Methods: In this cross sectional, descriptive study, 54 sites (PPD> 5mm) in 15 patients were considered for culture. Marginal gingiva was dried and sampling performed by paperpoint (#30). The selective medium for Aa, was Trypticase Soy Agar-Bacitracin- Vancomycin (TSBV) and for Pg was Brucella agar.Results were analyzed using Fisher and Chi-Square statistical tests.

Results: Thirteen patients or 38 sites (70.4%) were identified as Aa positive and 3 patients or 10 sites (18.4%) were Pg positive. There was no significant relation between the presence of Aa and sex or age (P=0.086). Pg was more prevalent in men compared with women (P<0.0001) but with regard to age there was no statistical difference between men and women. Aa had a significant positive correlation with PPD (P=0.002), which was not true for Pg. In addition, the number of positive sites showed a significant negative correlation with the number of family members.

Conclusion: Based on the present study, the prevalence of Aa in deep pockets in patients with AP is higher than Pg.

Background and Aims: Periodontitis is one of the most common oral diseases with the various incidence rates in different populations. A number of bacteria are considered as the major etiologic agents of periodontitis. The aim of the present study was to determine the prevalence of periodontopathogen bacteria in patients using both PCR and culture techniques.
Materials and Methods: In this study, one-hundred patients (including 62 females and 38 males with an average age of 49±11.5 years) with adult periodontitis referred to periodontics department of School of Dentistry/Tehran University of Medical Sciences were investigated. The samples were taken and sent immediately to the laboratory for culture and molecular evaluation. The PCR was performed using specific primers and the statistical analysis of data was performed using SPSS statistic software and McNemar test.
Results: The results demonstrated that the total detection rate in culture method was 64%. The rate of Aggregatibacter actinomycetemcomitans (Aa) was 28% which was significantly higher than that of Porphyromonas gingivalis (Pg) (6%) and Prevotella intermedia (Pi) (3%). 27% of cases showed mixed bacterial growth. 65% of patients were positive using molecular method. The rate of Aa (30%) was significantly higher than that of Pg (7%) and Pi (5%). The mixed PCR positive rate containing of Aa, Pg and Pi was (23%).
Conclusion: In this study, it was found that most of the bacteria isolated using culture and molecular methods were Aa, Pg and Pi, respectively. Although the detection frequencies of both techniques were similar, the specificity, sensitivity and bacterial detection speed of the PCR technique is obviously higher. Therefore, the use of molecular techniques is strongly recommended. However, both techniques seem to be suitable for microbiological diagnostics.

  Background and Aims : Antibiotics are commonly used for controlling the growth of porphyromons gingivalis (P.g) which is one of the most important etiologic factors in the periodontal diseases. Different side effects of synthetics and chemical drugs such as increasing the drug resistancy in the human pathogens have led to study on the herbal antibacterial effect. The aim of this study was to evaluate the antibacterial effect of cinnamon on the growth of porphyromons gingivalis in chronic periodontitis patients with deep pockets.

  Materials and Methods: In this experimental study, samples were provided from patients having pockets. After culturing the microorganism and diagnosis of P.g by gram staining and biochemical tests, cinnamon in different concentrations (10, 50, 100, 250, 500, 750 and 1500 mg/ml ) with oil solvent were prepared and placed by disks in the cultures medium. Positive controls were amoxicillin, metronidazole, ciprofloxacin, amikacin and gentamycin . Oil was negative control. Then the plates were incubated for 24 hours in 37 ⁰ C and then non-growth halos by disk diffusion method, MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were determined. Data were analyzed using One-way ANOVA test.

  Results: The results showed that the cinnamon at the concentration of MIC=750 mg/ml had the inhibitory effects of bacteria and at the concentration of MIC=1500 mg/ml had killing effect. However, this antibacterial effect compared with commonly used antibiotics (amoxicillin, metronidazole), was much weaker (P<0.001).

  Conclusion: Cinnamon showed an antimicrobial effect on porphyromonas gingivalis in chronic periodontitis patients with deep pockets.