M. Ramezaninan ,
Volume 15, Issue 4 (1-2003)
Abstract
Infection control, in all surgery operations, is of high importance. Although a lot of advances have been made, but it is still necessary to observe methods for the prevention of spreading infection, especially for dentists who perform several minor surgeries in the office. As a result, investigating aerobic bacterial contamination of metal instruments, before application, should be paid special attention. The aim of this study was to investigate the aerobic bacterial contamination on metal instrument before oral surgery. Metal instruments, used in surgery and non surgery operations in 4 departments of dental faculty-oral and maxillofacial surgery, endodontics, periodontics, autoclave room with the sampling volume of 80 were selected randomly. After sterilization of all samples under specific conditions, they were transferred to microbiological laboratory of hygiene faculty and were cultured and incubated under 37° temperature for 24 hours. According to the results of culturing, just one case, affected with staphylococcus epidermis, was observed. Sterilization condition, in hygiene faculty, comparing to previous studies, shows 20% progress.
F. Amini, Gh. Ramezanei,
Volume 22, Issue 2 (11-2009)
Abstract
Background and Aim: In treatment of posterior crossbite awareness of the effects of Haas and Hyrax rapid maxillary expanders (RME) on nasal cavity will help the clinician to select the better appliance. This study was carried out to compare the effects of Haas and Hyrax expanders on the nasal cavity of patients treated for posterior crossbite.
Materials and Methods: A clinical trial study was designed on posteroanterior (PA) cephalograms of 30 subjects to evaluate the nasal cavity width of 14 subjects (8 female & 6 male) with mean chronological age of 12± 2years who received RME with Hyrax type and 16 subjects (9 female & 7 male) with mean chronological age of 11±1.6 years who received Haas type palatal expander. Paired t-test was used to analyze the outcomes of expansion in each group. Student t-test was used to compare Haas and Hyrax groups.
Results: The mean value of screw expansion was 9±2 mm in both groups. In Hyrax group nasal cavity width
(Nc-cN) increased from 29.2 ±1.94 mm to 31.7 ±1.93mm (p= 0.001) and In Haas group it was increased from 27.75± 2.21 mm to 29.35 ± 2.26 mm(p= 0.043). When two groups were compared to each other, statistically this increase was more significant in Hyrax than in the Haas group (p=0.038).
Conclusion: In this study RME affected geometry of the nasal cavity by increasing the nasal cavity width. However In our sample, Hyrax appliance demonstrated better performance over the Haas appliance in all variables.
Amir Aliramezani, Mohammad Hosein Salari, Mohammad Reza Pourmand, Zeinab Kadkhoda, Abbas Foroushani, Farzaneh Aminharati, Sedigheh Ghourchian, Zahra Pakbaz, Saeed Eshraghi,
Volume 25, Issue 3 (7-2012)
Abstract
Background and Aims: Periodontitis is one of the most common oral diseases with the various incidence rates in different populations. A number of bacteria are considered as the major etiologic agents of periodontitis. The aim of the present study was to determine the prevalence of periodontopathogen bacteria in patients using both PCR and culture techniques.
Materials and Methods: In this study, one-hundred patients (including 62 females and 38 males with an average age of 49±11.5 years) with adult periodontitis referred to periodontics department of School of Dentistry/Tehran University of Medical Sciences were investigated. The samples were taken and sent immediately to the laboratory for culture and molecular evaluation. The PCR was performed using specific primers and the statistical analysis of data was performed using SPSS statistic software and McNemar test.
Results: The results demonstrated that the total detection rate in culture method was 64%. The rate of Aggregatibacter actinomycetemcomitans (Aa) was 28% which was significantly higher than that of Porphyromonas gingivalis (Pg) (6%) and Prevotella intermedia (Pi) (3%). 27% of cases showed mixed bacterial growth. 65% of patients were positive using molecular method. The rate of Aa (30%) was significantly higher than that of Pg (7%) and Pi (5%). The mixed PCR positive rate containing of Aa, Pg and Pi was (23%).
Conclusion: In this study, it was found that most of the bacteria isolated using culture and molecular methods were Aa, Pg and Pi, respectively. Although the detection frequencies of both techniques were similar, the specificity, sensitivity and bacterial detection speed of the PCR technique is obviously higher. Therefore, the use of molecular techniques is strongly recommended. However, both techniques seem to be suitable for microbiological diagnostics.
